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  4. Human Ig gamma-3 chain C region(IGHG3) ELISA kit

Human Ig gamma-3 chain C region(IGHG3) ELISA kit

  • 中文名稱：
    人Igγ3鏈C區(qū)(IGHG3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011158HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人Igγ3鏈C區(qū)(IGHG3)酶聯(lián)免疫試劑盒（CSB-EL011158HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IGHG3含量。IGHG3（免疫球蛋白重鏈恒定區(qū)3）是一種免疫球蛋白的重鏈恒定區(qū)，參與免疫應(yīng)答。研究顯示，IGHG3在肺結(jié)核病患者血清中的含量顯著高于健康對照組，可能與肺結(jié)核病的發(fā)病機(jī)制相關(guān)。其具體作用機(jī)制尚在研究中，但已有研究表明，IGHG3可能作為潛在生物標(biāo)志物，有助于肺結(jié)核病的診斷。試劑盒檢測范圍為2.7 ng/mL-2000 ng/mL，該產(chǎn)品適用于科研領(lǐng)域中對IgG3亞型表達(dá)水平的動態(tài)監(jiān)測，如探索自身免疫疾病病理機(jī)制、評估疫苗免疫效果、研究免疫調(diào)節(jié)藥物干預(yù)后的體液免疫變化等，為免疫學(xué)研究和生物標(biāo)志物發(fā)現(xiàn)提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IGHG3Immunoglobulin heavy constant gamma 3 ELISA Kit; HDC ELISA Kit; Heavy chain disease protein ELISA Kit; Ig gamma-3 chain C region ELISA Kit
  • 縮寫：
    IGHG3
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    2.7 ng/mL-2000 ng/mL
  • 靈敏度：
    2.7 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IGHG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %99
    Range %93-106
    1:2000Average %90
    Range %85-95
    1:4000Average %87
    Range %82-91
    1:8000Average %93
    Range %86-98
  • 回收率：
    The recovery of human IGHG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-94
    EDTA plasma (n=4)9590-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    20000.173 0.172 0.1725
    6670.259 0.246 0.2525
    2220.367 0.344 0.3555
    740.488 0.459 0.4735
    250.633 0.624 0.6285
    8.20.789 0.855 0.822
    2.71.029 1.033 1.031
    01.697 1.748 1.7225
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
  • 亞細(xì)胞定位：
    Secreted. Cell membrane.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 5527

    OMIM: 147120

    UniGene: Hs.510635