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  4. Human Inactive tyrosine-protein kinase 7(PTK7) ELISA kit

Human Inactive tyrosine-protein kinase 7(PTK7) ELISA kit

  • 中文名稱：
    人無活性的酪氨酸蛋白激酶7(PTK7)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL018996HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人無活性的酪氨酸蛋白激酶7(PTK7)酶聯(lián)免疫試劑盒（CSB-EL018996HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PTK7含量。PTK7是一個重要靶點。它在腫瘤等疾病的發(fā)生發(fā)展中可能發(fā)揮關鍵作用。研究發(fā)現(xiàn)其參與細胞信號傳導等過程，影響細胞的增殖、遷移等。對PTK7機制的研究有助于理解疾病發(fā)病機理，為開發(fā)針對性的診斷方法和治療藥物提供理論基礎。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于體外培養(yǎng)細胞模型、動物組織樣本或體液樣本中的PTK7蛋白表達研究，可應用于腫瘤生物學、干細胞分化、信號通路調控等基礎研究領域，為探索PTK7在疾病機制中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    8430404F20Rik ELISA Kit; CCK 4 ELISA Kit; CCK-4 ELISA Kit; Colon carcinoma kinase 4 ELISA Kit; Human colon carcinoma kinase 4 (CCK4) mRNA complete cds ELISA Kit; Inactive tyrosine-protein kinase 7 ELISA Kit; mPTK7/CCK4 ELISA Kit; OTTHUMP00000217016 ELISA Kit; Protein tyrosine kinase PTK 7 ELISA Kit; Protein-tyrosine kinase 7 ELISA Kit; Pseudo tyrosine kinase receptor 7 ELISA Kit; PTK 7 ELISA Kit; PTK7 ELISA Kit; PTK7 protein tyrosine kinase 7 ELISA Kit; PTK7 protein tyrosine kinase ELISA Kit; PTK7_HUMAN ELISA Kit; Serum response factor ELISA Kit; Srf ELISA Kit; Tyrosine-protein kinase-like 7 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.125 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PTK7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 89    
    Range % 84-95    
    1:2 Average % 92    
    Range % 87-101    
    1:4 Average % 98    
    Range % 94-104    
    1:8 Average % 98    
    Range % 93-105    
  • 回收率：
    The recovery of human PTK7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 93-102    
    EDTA plasma (n=4) 91 84-97    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    800 2.274 2.265 2.270 2.161    
    400 1.779 1.812 1.796 1.687    
    200 1.134 1.158 1.146 1.037    
    100 0.660 0.712 0.686 0.577    
    50 0.385 0.410 0.398 0.289    
    25 0.233 0.235 0.234 0.125    
    12.5 0.174 0.187 0.181 0.072    
    0 0.107 0.110 0.109 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：人

稀釋比：沒有稀釋

產(chǎn)品評價： 我用這款試劑盒做的血清中PTK7含量檢測，標準曲線做的不錯，但是樣品血清中含量太少，可能是放太久的原因，所以未能檢測到，產(chǎn)品沒問題。

By 劉老師

相關問答

■ 常見問題解答
Q:

Customer would like to know more about the immunising peptide/antigen
for raising antibodies

A:
Thanks for your inquiry!
CSB-EL018996HU
Capture antibody is rabbit polyclonal antibody and detection antibody is rabbit polyclonal antibody.
Immunogen is 293 cell derived recombinant protein.( extracellular domain)
Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能：
    Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
  • 基因功能參考文獻：
    1. miR2055p is involved in the proliferation, migration and invasion of colorectal cancer cells through inhibiting PTK7. PMID: 29488611
    2. Higher expression of PTK7 significantly indicates worse prognosis in human malignancies PMID: 28924970
    3. Inhibition of PTK7 by siRNA treatment significantly decreases the viability of atypical teratoid rhabdoid tumors (ATRT)patient-derived tumor cell lines.These studies provide the groundwork for future preclinical in vivo studies aiming to investigate the efficacy of PTK7 inhibition on ATRT tumor growth PMID: 28442586
    4. PTK7 localization and protein stability is affected by canonical Wnt ligands. PMID: 28420671
    5. These findings demonstrate that PTK7 upregulates MMP9 through activation of AP-1 and NF-kappaB and, thus increases invasive properties of ESCC cells. PMID: 27689325
    6. PTK7 overexpression is associated with esophageal squamous cell carcinoma. PMID: 28545451
    7. Our data indicate that PTK7 protein expression is associated with the prognosis of oral tongue squamous cell carcinoma PMID: 28547980
    8. PTK7 expression was correlated with tumor differentiation (P=0.027), lymph node metastasis (P=0.005), distant metastasis (P=0.001) and TNM stage (P=0.028) of colorectal cancer patients PMID: 27499181
    9. High PTK7 expression is associated with radioresistance in esophageal squamous cell carcinoma. PMID: 27557627
    10. PTK7 enriches in self-renewing, multipotent stem cells of the human colon. PMID: 26549850
    11. findings implicate PTK7 as a risk factor for NTDs and provide additional evidence for a pathogenic role of PCP signaling in these malformations PMID: 26368655
    12. overexpression in PTK7-negative cancer cells led to increased metastatic events. PTK7 expression thus represents a potential prognostic biomarker and a novel therapeutic target in CRC. PMID: 25962058
    13. data demonstrates that PTK7 regulates the activity of KDR biphasically by inducing oligomerization of KDR molecules at lower concentrations and by surrounding KDR molecules at higher concentrations. PMID: 25986862
    14. PTK7 regulates Id1 expression in CD44-high glioma cell lines. Targeting PTK7 could be an effective strategy for treating glioma with high CD44 expression. PMID: 25204555
    15. The results suggest that N-acylethanolamine acid amidase and protein tyrosine kinase 7 may be used as potential tissue biomarkers to avoid overtreatment of non-aggressive prostate cancer PMID: 24741114
    16. Expression level of PTK7 was significantly decreased from benign to malignant ovarian epithelial tumors. survival analysis showed that patients with negative expression of PTK7 protein had poorer outcome than those with positive expression. PMID: 25550828
    17. PTK7 acts as a cancer-related gene and may be a potent prognostic marker for Hepatocellular carcinoma(HCC). PMID: 25796105
    18. results provide convincing evidence that both PTK7 expression and proteolysis, rather than the level of the cellular full-length PTK7 alone, contribute to efficient directional cell motility and metastasis in cancer PMID: 25006253
    19. PTK7 expression plays an important role in the invasiveness of intrahepatic cholangiocarcinoma (ICC) cells and leads to a poor prognosis in ICC patients. PMID: 24587299
    20. PTK 7 is a transforming gene and prognostic marker for breast cancer and nodal metastasis involvement. PMID: 24409301
    21. This work defines PTK7 as a highly and specifically expressed gene in adenocarcinoma and a potential therapeutic target in this subset of NSCLC. PMID: 24654231
    22. Increased expression of PTK7 was inversely correlated with overall survival. PMID: 23663482
    23. in patients treated by chemotherapy, patients with PTK7-negative tumors seemed to have better DFS than those with PTK7-positive tumors, particularly for patients treated with only anthracycline-therapy drugs PMID: 24023307
    24. PTK7 is a novel marker of epithelial to mesenchymal transitions PMID: 23209741
    25. cleavage of the PTK7 ectodomain by an ADAM proteinase was coupled with the membrane type-1 matrix metalloproteinase (MT1-MMP) cleavage of the PKP(621) downward arrowLI site in the seventh Ig-like domain of PTK7. PMID: 23095747
    26. PTK7 has utility as a biomarker for detecting minimal residual disease of T cell acute lymphoblastic leukemia in the bone marrow PMID: 22898210
    27. a novel role for PTK7 in the tumorigenesis via generation of PTK7-CTF2 by sequential cleavage of ADAM17 and gamma-secretase. PMID: 22665490
    28. aberrations in the membrane type 1 matrix metalloproteinase/PTK7 axis are detrimental to cell movements that shape the body plan PMID: 21518755
    29. Observations may indicate a role for PTK7 in cell proliferation and cell apoptosis and may provide a potential therapeutic pathway for the treatment of a variety of cancers. PMID: 21103379
    30. Protein Tyrosine Kinase 7 is required for beta-catenin-dependent transcriptional events induced by canonical Wnt ligands PMID: 21132015
    31. Results indicate that PTK7 is a major target of MT1-MMP. PMID: 20837484
    32. PTK7 is a planar cell polarity component expressed in the myeloid progenitor compartment that conveys promigratory and antiapoptotic signals into the cell and represents an independent prognosis factor in patients treated with induction chemotherapy. PMID: 20558616
    33. cloning, sequencing and expression of gene and its splice variants PMID: 12427550
    34. Lack of a thermodynamically meaningful self-association propensity for the CCK4 transmembrane domains demonstrates that the conserved GxxxG motif is not sufficient to drive CCK4 transmembrane helix-helix interactions. PMID: 15683231
    35. mechanism of PTK7 upregulation in colorectal cancer PMID: 17671748
    36. PTK7 plays an important role not only in tube formation, migration, and invasion of endothelial cells but also in angiogenesis. PMID: 18471990

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  • 亞細胞定位：
    Membrane; Single-pass type I membrane protein. Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
  • 組織特異性：
    Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9618

    OMIM: 601890

    KEGG: hsa:5754

    STRING: 9606.ENSP00000230419

    UniGene: Hs.90572