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  4. Human Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) ELISA kit

Human Inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) ELISA kit

  • 中文名稱：
    人肌苷5-磷酸脫氫酶2 IMPDH2酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E17855h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人肌苷5-磷酸脫氫酶2 IMPDH2酶聯(lián)免疫試劑盒（CSB-E17855h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IMPDH2含量。IMPDH2 是重要靶點(diǎn)。它在細(xì)胞增殖、免疫調(diào)節(jié)等生理過程中發(fā)揮關(guān)鍵作用。研究中發(fā)現(xiàn)抑制 IMPDH2 能影響核苷酸合成，進(jìn)而干擾腫瘤細(xì)胞生長和病毒復(fù)制，在腫瘤治療和抗病毒治療領(lǐng)域是研究熱點(diǎn)，為相關(guān)疾病治療提供新思路。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，支持科研人員開展嘌呤代謝相關(guān)機(jī)制研究、抗腫瘤藥物篩選或免疫調(diào)節(jié)研究等實(shí)驗(yàn)。兼容多種生物樣本類型，尤其適用于體外培養(yǎng)細(xì)胞模型的代謝狀態(tài)評估或疾病動(dòng)物模型中 IMPDH2 的動(dòng)態(tài)監(jiān)測，為探索靶點(diǎn)在疾病發(fā)生發(fā)展中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IMDH2_HUMAN ELISA Kit; IMP (inosine monophosphate) dehydrogenase 2 ELISA Kit; IMP dehydrogenase 2 ELISA Kit; IMP oxireductase 2 ELISA Kit; IMPD 2 ELISA Kit; IMPD2 ELISA Kit; IMPDH 2 ELISA Kit; IMPDH II ELISA Kit; IMPDH-II ELISA Kit; Impdh2 ELISA Kit; Inosine 5' monophosphate dehydrogenase 2 ELISA Kit; Inosine 5' phosphate dehydrogenase 2 ELISA Kit; Inosine monophosphate dehydrogenase type II ELISA Kit; Inosine-5''-monophosphate dehydrogenase 2 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IMPDH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %88-98
    1:2Average %97
    Range %93-102
    1:4Average %94
    Range %89-98
    1:8Average %87
    Range %82-91
  • 回收率：
    The recovery of human IMPDH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-102
    EDTA plasma (n=4)8882-92
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.632 2.510 2.571 2.448
    7501.476 1.519 1.498 1.375
    3750.867 0.820 0.844 0.721
    187.50.473 0.465 0.469 0.346
    940.305 0.311 0.308 0.185
    470.258 0.246 0.252 0.129
    23.50.189 0.185 0.187 0.064
    00.124 0.122 0.123
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
  • 基因功能參考文獻(xiàn)：
    1. Our study demonstrates IMPDH2 may be served as an independent prognostic biomarker for NPC patients, in which high IMPDH expression suggests poor prognosis of NPC patients. PMID: 28389646
    2. inosine-5'-monophosphate dehydrogenase 2 (IMPDH2) is an intracellular target of the PPIA-Sanglifehrin A binary complex. PMID: 28076787
    3. Rod and ring formation might be an adaptive homeostatic mechanism, allowing IMPDH to sense changes in the one-carbon folate pathway. PMID: 27343244
    4. Taking together, these results indicate that natural product myricetin exhibits potent anti-leukemia activity by interfering with purine nucleotides biosynthetic pathway through the suppression of hIMPDH1/2 catalytic activity. PMID: 27378425
    5. The findings suggest that overexpressed IMPDH2 can be used as a biomarker for kidney and bladder cancer diagnosis and is a potential therapeutic target for the diseases PMID: 25465060
    6. IMPDH2 is directly involved in the development of chemoresistance and radioresistance in osteosarcoma cells. PMID: 25392102
    7. Compared with non-cancerous prostate tissues, IMPDH2 mRNA and protein expression levels were both significantly upregulated in prostate cancer tissues. PMID: 24659377
    8. the localization of IMPDH2 inside the nucleus of human cells as well as the ultrastructure of R&R inclusions PMID: 24980853
    9. Rods and rings (RR) are protein assemblies composed of CTPS1 and IMPDH2. Glutamine deprivation initiates reversible assembly of mammalian rods and rings. PMID: 24477477
    10. An insight to the dynamics of conserved water-mediated salt bridge interaction and interdomain recognition in hIMPDH isoforms. PMID: 22928911
    11. Role of salt bridge dynamics in inter domain recognition of human IMPDH isoforms: an insight to inhibitor topology for isoform-II. PMID: 22066532
    12. This ethnic-related data might contribute to a better understanding of the variability in clinical outcome and/or dose adjustments of drugs that are IMPDH inhibitors such as mycophenolic acid. PMID: 21181270
    13. the 3757C allele was associated with higher lymphocyte counts and a reduced incidence of lymphopenia among kidney allograft recipients PMID: 21996196
    14. Anti-IMPDH2 may cause difficulties in interpretation of immunofluorescence patterns in routine autoantibody testing. PMID: 22029192
    15. IMPDHII plays an important role in the negative regulation of TLR2 signaling by modulating PI3K activity. PMID: 21460227
    16. in the absence of ligand the mobilities of "flap" and "loop" regions of IMPDH-II (1B3O) structures are mostly controlled by the four (W 1, W 2, W 3, and W 4) conserved water molecular centers and their dynamics PMID: 20135634
    17. resequenced IMPDH1 and IMPDH2 using DNA from 288 individuals from three ethnic groups and performed functional genomic studies of the sequence variants observed; identfied 25 SNPs, 24 novel, in IMPDH2 PMID: 20718729
    18. IMPDH2 is directly involved in the development of chemoresistance in osteosarcoma cells PMID: 20808934
    19. IMPDH2 genetic polymorphism was investigated in 96 individuals of Caucasian origin. PMID: 19810816
    20. Pharmacogenetic analysis of IMPDH II may represent a novel approach to mycophenolate mofetil therapeutic monitoring. PMID: 15621150
    21. Nine genetic variants were identified in the IMPDH2 gene, with frequencies of the rarer alleles ranging from 0.5 to 10.2%. PMID: 17496727
    22. IMPDH2 is a marker gene useful for stratifying osteosarcoma patients into low- and high-risk groups and predicting therapy outcome. PMID: 17660802
    23. The effect of diabetes mellitus on mycophenolic acid pharmacokinetics and catalytic activity of inosine monophosphate dehydrogenase 2 was investigated in maintenance kidney transplant recipients. PMID: 18043470
    24. The synthesis and activity of several novel inhibitors of IMPDH2 are reported. PMID: 18583139
    25. Analysis of in vivo C-MYC interactions with TS, IMPDH2 and PRPS2 genes confirmed that they are direct C-MYC targets. PMID: 18677108
    26. The expression of IMPDH2 in tumor tissue was significantly higher in patients with colorectal cancer than that in healthy subjects. PMID: 19597826
    27. the IMPDH type II 3757T > C polymorphism is associated with an increased IMPDH activity in MMF-treated renal transplant patients PMID: 19617864

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytosol.
  • 蛋白家族：
    IMPDH/GMPR family
  • 組織特異性：
    IMPDH1 is the main species in normal leukocytes and IMPDH2 predominates over IMPDH1 in the tumor.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6053

    OMIM: 146691

    KEGG: hsa:3615

    STRING: 9606.ENSP00000321584

    UniGene: Hs.654400