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Human Integrin beta-7(ITGB7) ELISA kit

  • 中文名稱:
    人整合素β-7(ITGB7)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL011891HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人整合素β-7(ITGB7)酶聯(lián)免疫試劑盒(CSB-EL011891HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的ITGB7含量。ITGB7屬于整合素家族成員,在免疫系統(tǒng)中發(fā)揮重要作用。它能介導(dǎo)細(xì)胞與細(xì)胞、細(xì)胞與細(xì)胞外基質(zhì)間相互作用。研究中發(fā)現(xiàn)其與炎癥性疾病、腫瘤轉(zhuǎn)移等密切相關(guān),通過干預(yù)ITGB7相關(guān)信號(hào)通路,有望為相關(guān)疾病治療提供新思路。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL,適用于科研領(lǐng)域中對(duì)腸道相關(guān)疾病模型、免疫細(xì)胞遷移機(jī)制或黏膜屏障功能的研究,例如通過檢測(cè)炎癥性腸病動(dòng)物模型血清ITGB7水平變化,或分析腸道組織勻漿中該蛋白的表達(dá)動(dòng)態(tài),為探索免疫調(diào)控網(wǎng)絡(luò)提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    ITGB7; Integrin beta-7; Gut homing receptor beta subunit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human ITGB7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    102

    Range %

    95-108

    1:2

    Average %

    97

    Range %

    92-103

    1:4

    Average %

    91

    Range %

    87-90

    1:8

    Average %

    93

    Range %

    88-99

  • 回收率:

    The recovery of human ITGB7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    86-100

    EDTA plasma (n=4)

    96

    90-101

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    3.028

    2.934

    2.981

    2.843

    10

    2.523

    2.667

    2.595

    2.457

    5

    1.888

    1.928

    1.908

    1.771

    2.5

    1.182

    1.229

    1.206

    1.068

    1.25

    0.685

    0.721

    0.703

    0.566

    0.625

    0.421

    0.433

    0.427

    0.290

    0.312

    0.276

    0.284

    0.280

    0.142

    0

    0.140

    0.135

    0.138

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
  • 基因功能參考文獻(xiàn):
    1. CCR9 and Integrin-beta7 expression has a differential effect on graft fate during acute graft-versus-host disease (GVHD) of the liver depending on the GVHD target tissue. PMID: 26348893
    2. Integrin alpha4beta7 binds gp120 but does not mediate HIV envelope-induced death signaling. PMID: 22822002
    3. Consistent with structural predictions, strain increases beta-integrin binding to FLNA, whereas it causes FilGAP to dissociate from FLNA, providing a direct and specific molecular basis for cellular mechanotransduction PMID: 21926999
    4. a role for integrin-beta7 in MM-cell adhesion, migration, and BM homing, and pave the way for a novel therapeutic approach targeting this molecule. PMID: 21474670
    5. Data show that integrin beta7 and CCR6 identify four distinct stages of memory CD4+ T cell differentiation. PMID: 21398606
    6. T cell stimulation leads to incresed phosphorylation of integrin beta 7 and reveals threonine phosphorylation of beta 7. PMID: 12682249
    7. bipolar metal ion cluster sites stabilize two alternative phases of adhesion PMID: 14608374
    8. In a chronic ileitis model, pathogenic CD4+ T cells use the integrin beta 7/MAdCAM-1 pathway in order to recirculate to the chronically inflamed small intestine. PMID: 15699171
    9. Integrin activation marker CD103 (alphaEbeta7) is expressed on Epstein-Barr virus-specific tonsil-resident (but not peripheral blood mononuclear leukocyte-derived) cytotoxic T lymphocytes. PMID: 16177076
    10. Beta7 integrin is a major galectin-1-glycosylated counterreceptor involved in immune developmental synapse formation. PMID: 16818733
    11. quasi-palindromic sequence YDRREY within the beta7 cytoplasmic tail constitutes a cell adhesion regulatory domain that modulates the interaction of beta7-expressing leukocytes with their endothelial and epithelial ligands PMID: 16874740
    12. Cadherin-E interaction with integrin alphaEbeta7 causes antitumor cytotoxic response by CD8+/CD103+ tumor-reactive T lymphocytes. PMID: 17325197
    13. Taken together, these data establish beta7 ITG as a new molecular target of PAHs, whose up-regulation by these environmental contaminants most likely requires activation of co-operative pathways involving both AhR and c-maf. PMID: 17490615
    14. analysis of the migfilin-filamin interaction and competition with integrin beta 7 tails PMID: 18829455
    15. Mechanical force applied to filamin can expose cryptic integrin binding sites. PMID: 19699211

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  • 亞細(xì)胞定位:
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族:
    Integrin beta chain family
  • 組織特異性:
    Expressed in a variety of leukocyte lines.
  • 數(shù)據(jù)庫鏈接:

    HGNC: 6162

    OMIM: 147559

    KEGG: hsa:3695

    STRING: 9606.ENSP00000267082

    UniGene: Hs.654470