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  4. Human Inter-alpha-trypsin inhibitor heavy chain H2(ITIH2) ELISA kit

Human Inter-alpha-trypsin inhibitor heavy chain H2(ITIH2) ELISA kit

  • 中文名稱：
    人間α-胰蛋白酶抑制劑重鏈H2(ITIH2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011895HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人間α-胰蛋白酶抑制劑重鏈H2(ITIH2)酶聯(lián)免疫試劑盒（CSB-EL011895HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ITIH2含量。試劑盒檢測(cè)范圍為3.12 μg/mL-200 μg/mL，適用于基礎(chǔ)醫(yī)學(xué)研究中ITIH2蛋白水平的定量分析，如炎癥性疾病機(jī)制探究、腫瘤微環(huán)境研究、代謝相關(guān)疾病動(dòng)物模型評(píng)估等科研場(chǎng)景，也可用于比較不同組織樣本中ITIH2的表達(dá)差異，為相關(guān)分子調(diào)控網(wǎng)絡(luò)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    AI747202 ELISA Kit; H2P ELISA Kit; IGHEP2 ELISA Kit; Inter alpha (globulin) inhibitor H2 ELISA Kit; Inter alpha globulin inhibitor H2 polypeptide ELISA Kit; Inter alpha trypsin inhibitor complex component II ELISA Kit; Inter alpha trypsin inhibitor heavy chain H2 ELISA Kit; Inter-alpha-inhibitor heavy chain 2 ELISA Kit; Inter-alpha-trypsin inhibitor complex component II ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H2 ELISA Kit; Intin2 ELISA Kit; ITI HC2 ELISA Kit; ITI heavy chain H2 ELISA Kit; ITI-HC2 ELISA Kit; Itih 2 ELISA Kit; Itih2 ELISA Kit; ITIH2_HUMAN ELISA Kit; OTTHUMP00000019058 ELISA Kit; OTTHUMP00000019059 ELISA Kit; OTTMUSP00000012019 ELISA Kit; RP23 119N4.3 ELISA Kit; Serum derived hyaluronan associated protein ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
  • 縮寫(xiě)：
    ITIH2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 μg/mL-200 μg/mL
  • 靈敏度：
    0.78 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %91
    Range %86-94
    1:10Average %97
    Range %90-104
    1:20Average %101
    Range %96-107
    1:40Average %89
    Range %84-94
  • 回收率：
    The recovery of human ITIH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9088-95
    EDTA plasma (n=4)10297-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2AverageCorrected
    2002.439 2.555 2.497 2.312
    1001.867 1.728 1.798 1.613
    501.221 1.265 1.243 1.058
    250.751 0.757 0.754 0.569
    12.50.412 0.422 0.417 0.232
    6.250.318 0.336 0.327 0.142
    3.120.272 0.281 0.277 0.092
    00.183 0.186 0.185
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
  • 基因功能參考文獻(xiàn)：
    1. It forms complex with hyaluronan and the comlex is a functional molecule involved in inflammation. PMID: 20297716
    2. TSG-6 acts as cofactor and catalyst in the production of IalphaI heavy chain x hyaluronan complexes PMID: 15840581
    3. Leukocytes infiltrated to the synovium were strongly positive for hyaluronic acid, SHAP, and CD44 on their surfaces, suggesting a role for the adhesion-enhancing effect of SHAP in inflammation. PMID: 16702221
    4. Increased levels of SHAP-HA complex in sera are possible predictive markers for cervical ripening in premature labor. PMID: 18382897
    5. serum-derived hyaluronan-associated protein-hyaluronan complex has a role in progression of ovarian cancer PMID: 18425383

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    ITIH family
  • 組織特異性：
    Plasma.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 6167

    OMIM: 146640

    KEGG: hsa:3698

    STRING: 9606.ENSP00000351190

    UniGene: Hs.75285