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  4. Human Inter-alpha-trypsin inhibitor heavy chain H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)ELISA kit

Human Inter-alpha-trypsin inhibitor heavy chain H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)ELISA kit

  • 中文名稱：
    人間-alpha-胰蛋白酶抑制劑重鏈H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17022h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人間-alpha-胰蛋白酶抑制劑重鏈H4(ITIH4/IHRP/ITIHL1/PK120/PRO1851)酶聯(lián)免疫試劑盒（CSB-E17022h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ITIH4含量。當前所給信息未包含 ITIH4 相關背景及研究機制內容，無法總結。若你能提供更多有關 ITIH4 背景、研究機制的詳細文本，我會為你精煉總結成 100 字左右的段落。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，支持科研人員探究ITIH4在疾病發(fā)生發(fā)展中的作用機制、藥物干預效果評估或炎癥相關通路研究，為分子生物學、病理學及藥理學領域的基礎研究提供可靠工具，適用于體外定量分析多種生物樣本中ITIH4的表達水平。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    35 kDa inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; gp120 ELISA Kit; H4P ELISA Kit; IHRP ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; inter-alpha-trypsin inhibitor, heavy chain-like, 1 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; ITIH4 ELISA Kit; ITIH4_HUMAN ELISA Kit; ITIHL1 ELISA Kit; OTTHUMP00000197120 ELISA Kit; OTTHUMP00000197121 ELISA Kit; OTTHUMP00000213834 ELISA Kit; OTTHUMP00000213869 ELISA Kit; PK-120 ELISA Kit; PK120 ELISA Kit; Plasma kallikrein sensitive glycoprotein 120 ELISA Kit; PRO1851 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 82-97  
    1:2 Average % 97  
    Range % 92-102  
    1:4 Average % 96  
    Range % 90-104  
    1:8 Average % 97  
    Range % 92-102  
  • 回收率：
    The recovery of human ITIH4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 106 98-110  
    EDTA plasma (n=4) 95 91-105  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.539 2.438 2.489 2.329  
    20 2.047 1.946 1.997 1.837  
    10 1.312 1.301 1.307 1.147  
    5 0.755 0.766 0.761 0.601  
    2.5 0.463 0.452 0.458 0.298  
    1.25 0.337 0.326 0.332 0.172  
    0.625 0.242 0.245 0.244 0.084  
    0 0.160 0.159 0.160 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
  • 基因功能參考文獻：
    1. Low ITIH4 expression is associated with Hepatocellular Carcinoma. PMID: 28828637
    2. this report has further supported for associations of genetic variants in the ITIH4 and CALN1 genes with schizophrenia and provided the first evidence that the variants regulate ITIH4 AND CALN1 expression in the dorsolateral prefrontal cortex PMID: 26991396
    3. ITIH4 peptide isoform as a preterm birth biomarker and its associated SNP implications PMID: 26408095
    4. Serum ITIH4 may be a PM10-specific biomarker in COPD and may be related to inflammation. PMID: 25977605
    5. Four novel body mass index-associated loci near the KCNQ1(rs2237892), ALDH2/MYL2 (rs671, rs12229654), ITIH4 (rs2535633) and NT5C2 (rs11191580) genes are identified in East Asian-ancestry populations. PMID: 24861553
    6. Worse survival among HCC patients with low ITIH4. PMID: 24836184
    7. Isoform-specific ITIH4 glycosylation and utilization of O-glycosylation sites on ITIH4 differs between cell lines and serum. PMID: 24884609
    8. Expression of the 85 kDa ITIH4 was substantial in amyotrophic lateral sclerosis compared with controls or patients with muscular dystrophy, Alzheimer diseases, or Parkinson diseases PMID: 23436019
    9. A truncated fragment of inter-alpha-trypsin inhibitor heavy chain 4 was the sole protein found to be significantly enhanced in the prostate cancer patients compared to the controls. PMID: 23417432
    10. Findings suggest that ITI-H4 expression may be used as a biomarker, which could facilitate the development of novel diagnostic and therapeutic tools. PMID: 21331437
    11. Genetic variation at ITIH4 locus is one of the likely candidate determinants for plasma cholesterol metabolisms PMID: 14661079
    12. ITIH4 and MTJ1 co-immunoprecipitate from total liver protein extracts and SANT domain of HTJ1 protects the ITIH4(588-930) recombinant fragment PMID: 16271702
    13. The inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4) protein was significantly present more in interstial cystitis than controls PMID: 18455532
    14. ITIH4 is an anti-inflammatory protein, and suggests that further investigation into its potential use in the diagnosis and prognosis of acute ischemic stroke is warranted. PMID: 19263524

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    ITIH family
  • 組織特異性：
    Liver specific.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6169

    OMIM: 600564

    KEGG: hsa:3700

    STRING: 9606.ENSP00000266041

    UniGene: Hs.709406