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  4. Human Interferon alpha-21(IFNA21) ELISA kit

Human Interferon alpha-21(IFNA21) ELISA kit

  • 中文名稱：
    人干擾素α-21(IFNA21)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011039HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人干擾素α-21(IFNA21)酶聯(lián)免疫試劑盒（CSB-EL011039HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IFNA21含量。人干擾素α-21（IFNA21）是一種具有抗病毒和抗增殖活性的蛋白質(zhì)，通過(guò)結(jié)合細(xì)胞表面受體IFNAR1和IFNAR2，激活JAK-STAT信號(hào)通路，調(diào)節(jié)免疫反應(yīng)。其研究涉及基因改組技術(shù)，旨在提高其抗病毒和抗增殖活性，用于治療多種疾病。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品適用于科研領(lǐng)域?qū)Ω腥拘约膊?、自身免疫反?yīng)或腫瘤微環(huán)境等研究中IFNA21表達(dá)水平的定量分析，可用于評(píng)估病毒感染模型、免疫調(diào)節(jié)實(shí)驗(yàn)或藥物干預(yù)后生物樣本中IFNA21的動(dòng)態(tài)變化，為探索其生物學(xué)功能及作用機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    IFNA21 ELISA Kit; Interferon alpha-21 ELISA Kit; IFN-alpha-21 ELISA Kit; Interferon alpha-F ELISA Kit; LeIF F ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFNA21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %98
    Range %91-101
    1:2Average %104
    Range %98-107
    1:4Average %87
    Range %83-91
    1:8Average %94
    Range %90-97
  • 回收率：
    The recovery of human IFNA21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9184-94
    EDTA plasma (n=4)10196-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.749 2.701 2.725 2.576
    5001.752 1.786 1.769 1.620
    2501.177 1.124 1.151 1.002
    1250.729 0.712 0.721 0.572
    62.50.509 0.487 0.498 0.349
    31.20.350 0.343 0.347 0.198
    15.60.256 0.249 0.253 0.104
    00.148 0.150 0.149
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 5424

    OMIM: 147584

    KEGG: hsa:3452

    STRING: 9606.ENSP00000369574

    UniGene: Hs.113211