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  4. Human Interferon-induced 35 kDa protein(IFI35) ELISA kit

Human Interferon-induced 35 kDa protein(IFI35) ELISA kit

  • 中文名稱(chēng)：
    人干擾素誘導(dǎo)蛋白35kDa(IFI35)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011013HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人干擾素誘導(dǎo)蛋白35kDa(IFI35)酶聯(lián)免疫試劑盒（CSB-EL011013HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的IFI35含量。試劑盒檢測(cè)范圍為25 pg/mL-1600 pg/mL，本試劑盒適用于體外培養(yǎng)細(xì)胞模型、動(dòng)物實(shí)驗(yàn)組織樣本以及臨床前研究中的體液樣本分析，為探索IFI35在免疫調(diào)控機(jī)制、抗病毒反應(yīng)研究及藥物開(kāi)發(fā)中的生物學(xué)功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    FLJ21753 ELISA Kit; IFI 35 ELISA Kit; Ifi-35 ELISA Kit; Ifi35 ELISA Kit; IFP 35 ELISA Kit; IFP35 ELISA Kit; IN35 ELISA Kit; IN35_HUMAN ELISA Kit; Interferon induced 35 kDa protein ELISA Kit; Interferon-induced 35 kDa protein ELISA Kit; OTTHUMP00000236626 ELISA Kit; OTTHUMP00000236627 ELISA Kit
  • 縮寫(xiě)：
    IFI35
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFI35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 98
    Range % 96-102
    1:2 Average % 95
    Range % 89-100
    1:4 Average % 94
    Range % 90-98
    1:8 Average % 88
    Range % 86-95
  • 回收率：
    The recovery of human IFI35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 99-108
    EDTA plasma (n=4) 93 90-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.768 2.848 2.808 2.715
    800 1.622 1.709 1.666 1.573
    400 1.034 1.115 1.075 0.982
    200 0.669 0.655 0.662 0.569
    100 0.323 0.346 0.335 0.242
    50 0.227 0.222 0.225 0.132
    25 0.144 0.134 0.139 0.046
    0 0.096 0.090 0.093 　
    .
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as a signaling pathway regulator involved in innate immune system response. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation. In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production. In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion. Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines.
  • 基因功能參考文獻(xiàn)：
    1. IFI35 enhances proliferation of mesangial cells and is regulated by MBD2 in lupus nephritis. PMID: 28064541
    2. Damage-associated molecular patterns (DAMP) are important mediators of innate immunity. Here the authors show that N-myc and STAT interactor (NMI) and interferon-induced protein 35 (IFP35) act as DAMPs to promote inflammation by activating macrophages via the Toll-like receptor 4 and NF-kappaB pathways. PMID: 29038465
    3. Study found that TLR3 signaling induces the expression of IFI35, and IFI35 negatively regulates IFN-beta-P-STAT1-RIG-I-CXCL10/CCL5 axis in U373MG cells. This suggests that IFI35 expressed in astrocytes may play an important role in regulating the innate immune system in astrocytoma cells. PMID: 28109979
    4. Activation of TLR3 by poly IC induces the IFI35 expression in Mesangial Cells. Regional expression of IFI35 and its dysregulation may be involved in the pathogenesis of glomerular inflammation in CKD. PMID: 27639618
    5. Trim21 regulates Nmi-IFI35 complex-mediated inhibition of innate antiviral response PMID: 26342464
    6. Knockdown of IFP35 expression abolished pEGFP-N1-2C and pEGFP-N1-Nmi-induced activation of type I interferon promoters. PMID: 25085622
    7. IFI35 negatively regulates RIG-I antiviral signaling and supports vesicular stomatitis virus replication. PMID: 24371060
    8. IRF-1 also activates IFP35 expression in an IFN-gamma-inducible manner. PMID: 23226549
    9. Dissociation of the IFN-induced protein IFP35 from NMI is a newly defined specific cytoplasmic event occurring during apoptosis. PMID: 11911807
    10. The results provide a novel role of CKIP-1 in cytokine signaling response and the biochemical mechanism, by which two previously identified modulators IFP35 and Nmi are involved via interactions. PMID: 17197158
    11. IFP35 can interact with the bovine Tas (BTas) regulatory protein of bovine foamy virus; overexpression of IFP35 disturbs the ability of BTas to activate viral-gene transcription and inhibits viral replication. PMID: 18305040

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Secreted.
  • 蛋白家族：
    NMI family
  • 組織特異性：
    Expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 5399

    OMIM: 600735

    KEGG: hsa:3430

    STRING: 9606.ENSP00000395590

    UniGene: Hs.632258