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  4. Human Interferon kappa(IFNK) ELISA kit

Human Interferon kappa(IFNK) ELISA kit

  • 中文名稱：
    人干擾素κ(IFNK)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL011053HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人干擾素κ(IFNK)酶聯(lián)免疫試劑盒（CSB-EL011053HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNK含量。IFNK即干擾素κ，是I型干擾素家族成員。它具有抗病毒、抗腫瘤和免疫調(diào)節(jié)等潛在作用。其研究機制方面，能與細胞表面受體結(jié)合，激活相關(guān)信號通路，誘導(dǎo)多種抗病毒蛋白產(chǎn)生，發(fā)揮免疫調(diào)節(jié)活性，抵御病原體入侵與腫瘤發(fā)展。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于病毒感染機制、自身免疫疾病模型構(gòu)建、皮膚屏障功能研究等科研領(lǐng)域，還特別適用于免疫微環(huán)境分析、細胞因子網(wǎng)絡(luò)研究等基礎(chǔ)科研場景，能為探索IFNK在炎癥性疾病、腫瘤免疫調(diào)控及組織特異性免疫應(yīng)答中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IFN kappa ELISA Kit; IFN-kappa ELISA Kit; Ifnk ELISA Kit; IFNK_HUMAN ELISA Kit; Interferon kappa ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFNK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 89-97    
    1:2 Average % 85    
    Range % 80-90    
    1:4 Average % 90    
    Range % 85-94    
    1:8 Average % 98    
    Range % 94-103    
  • 回收率：
    The recovery of human IFNK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 101 97-105    
    EDTA plasma (n=4) 92 88-96    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.694 2.758 2.726 2.622    
    500 2.309 2.182 2.246 2.142    
    250 1.515 1.601 1.558 1.454    
    125 0.954 1.004 0.979 0.875    
    62.5 0.521 0.560 0.541 0.437    
    31.25 0.287 0.293 0.290 0.186    
    15.6 0.193 0.205 0.199 0.095    
    0 0.100 0.107 0.104 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon-stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin.
  • 基因功能參考文獻：
    1. investigation of common variable immunodeficiency (CVID) from 2 German families; report on the occurrence of a common and one novel truncating IFNK mutation in cases with CVID; the frequency distribution of c.30_31insTGTT in cases and controls as well as the observed segregation patterns in CVID families exclude IFNK mutations as major risk factor in CVID PMID: 28324805
    2. These results suggest that high-risk human papillomavirus 31 target interferon kappa to prevent Sp100 expression and identify Sp100 as an interferon-stimulated gene with anti-human papillomavirus activity. PMID: 26491169
    3. This study demonstrates that E2 proteins of high risk human papillomavirus reduce STING and IFN-kappa transcription. PMID: 24614210
    4. The viral E6 and E7 oncogenes are sufficient for interferon-kappa repression, with E6 being mainly responsible. PMID: 21849431
    5. IFN-kappa is down-regulated in cervical keratinocytes harboring HPV, which may be a contributing factor in the progression of a cervical lesion PMID: 20479716
    6. Studied IFNK single nucleotide polymorphisms in 3982 Systemic lupus erythematosus cases and 4275 controls. PMID: 20706608
    7. IFN-kappa is able to directly modulate cytokine release from monocytes and dendritic cells, inhibit inducible IL-12 release from monocytes, and bind strongly to heparin. PMID: 12391192
    8. This is the first report showing an epigenetic silencing of type I IFN after HPV16 oncogene expression PMID: 19887612

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 組織特異性：
    Expressed in keratinocytes, monocytes and in resting dendritic cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 21714

    OMIM: 615326

    KEGG: hsa:56832

    STRING: 9606.ENSP00000276943

    UniGene: Hs.591083