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  4. Human Interleukin 22(IL-22) ELISA KIT

Human Interleukin 22(IL-22) ELISA KIT

  • 中文名稱：
    人白介素22(IL-22)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13418h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人白介素22(IL-22)酶聯(lián)免疫試劑盒（CSB-E13418h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL22含量。IL-22是一種細(xì)胞因子，主要由Th17等細(xì)胞產(chǎn)生。它通過與受體結(jié)合激活下游信號通路。IL-22在黏膜屏障維護、組織修復(fù)及炎癥反應(yīng)中發(fā)揮重要作用，其異常表達與多種疾病如自身免疫病、感染性疾病發(fā)病機制有關(guān)，是研究熱點。試劑盒檢測范圍為78 pg/ml- 5000 pg/ml，靈敏度為19.5 pg/ml?？蒲蓄I(lǐng)域可應(yīng)用于免疫調(diào)節(jié)機制研究、炎癥模型構(gòu)建、藥物干預(yù)效果評估等方向，為探索IL - 22在組織穩(wěn)態(tài)維持、病原體防御及上皮修復(fù)中的分子機制提供可靠工具。適用于實驗室常規(guī)檢測及高通量樣本分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cytokine Zcyto18 ELISA Kit; IL 10 related T cell derived inducible factor ELISA Kit; IL 21 ELISA Kit; IL 22 ELISA Kit; IL D110 ELISA Kit; IL TIF ELISA Kit; IL-10-related T-cell-derived-inducible factor ELISA Kit; IL-22 ELISA Kit; IL-TIF ELISA Kit; IL21 ELISA Kit; Il22 ELISA Kit; IL22_HUMAN ELISA Kit; ILD110 ELISA Kit; ILTIF ELISA Kit; Interleukin 10 related T cell derived inducible factor ELISA Kit; interleukin 21 ELISA Kit; Interleukin 22 ELISA Kit; Interleukin-22 ELISA Kit; MGC79382 ELISA Kit; MGC79384 ELISA Kit; TIFa ELISA Kit; TIFIL 23 ELISA Kit; TIFIL23 ELISA Kit; UNQ3099/PRO10096 ELISA Kit; zcyto18 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 pg/ml - 5000 pg/ml
  • 靈敏度：
    19.5 pg/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    103

    Range %

    99-109

    1:2

    Average %

    109

    Range %

    105-115

    1:4

    Average %

    98

    Range %

    91-105

    1:8

    Average %

    93

    Range %

    86-100

  • 回收率：

    The recovery of human IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    107

    103-111

    EDTA plasma (n=4)

    99

    92-106

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.994

    3.064

    3.029

    2.921

    2500

    2.338

    2.307

    2.322

    2.215

    1250

    1.400

    1.358

    1.379

    1.272

    625

    0.719

    0.729

    0.724

    0.617

    312

    0.371

    0.391

    0.381

    0.274

    156

    0.271

    0.263

    0.267

    0.160

    78

    0.196

    0.184

    0.190

    0.083

    0

    0.110

    0.105

    0.107

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-22 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-22 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytokine that contributes to the inflammatory response in vivo.
  • 基因功能參考文獻：
    1. these findings show that CD147 is a novel and key mediator of IL-22-induced psoriatic alterations in the epidermis and might be a therapeutic target in patients with psoriasis. PMID: 28272440
    2. we have shown that variants of the IL22 gene may play an important role in the pathogenesis of CM and that these variants of IL22 are associated with an aggravation of malaria. The rs2227473 risk allele for CM, the T allele, is associated with higher levels of IL-22 production, suggesting that IL-22 contributes to CM. PMID: 28139719
    3. This study highlights a mechanism through which the IL-22 pathway facilitates the human intestinal epithelium to control microbial infection. PMID: 30217896
    4. rs1179251 polymorphism may be a risk factor for cancer [meta-analysis] PMID: 29761647
    5. the expression of miR-548a-3p is upregulated in IL-22 mediated keratinocyte proliferative disorder like psoriasis. PMID: 29181737
    6. IL-22 is involved in A549 cell resistance to paclitaxel through regulating cell apoptosis via the JNK signaling pathway. PMID: 29723165
    7. we summarize the current knowledge on the roles of IL-22 in the various pancreatic pathogenesis, providing insights into the underlying cellular and signaling mechanisms--{REVIEW} PMID: 29502986
    8. IL22 is upregulated by atorvastatin, losartan and captopril and its polymorphism may have a role in hypertension and coronary artery disease PMID: 29981321
    9. Increased IL-22 urinary level was not associated with candida urinary tract infection. PMID: 29421775
    10. The level of lncRNA H19 is increased in inflamed intestinal tissues from mice and patients. The inflammatory cytokine IL22 induces expression of H19 in IECs, which is required for intestinal epithelial proliferation and mucosal healing. H19 lncRNA appears to inhibit p53 protein and microRNA 34a and let-7 to promote proliferation of IECs and epithelial regeneration. PMID: 29621481
    11. hidradenitis suppurativa keratinocytes exhibited lower amounts of IL-22 PMID: 28972431
    12. Study shows that in Chinese subjects, the plasma concentration of interleukin-22 (IL-22) is profoundly associated with susceptibility to impaired fasting glucose (IFG) and type 2 diabetes, and decreased plasma IL-22 level is a potential trigger of IFG and type 2 diabetes. PMID: 28170163
    13. effect of IL-22 on Intestinal Epithelial Cells responses may not be in inducing CXCL8 by itself, but in enhancing TNF-alpha- and IL-1-induced CXCL8 secretion to augment the contribution of IECs to local inflammatory responses. PMID: 28656529
    14. Despite the presence of all Notch pathway molecules in the kidney and a model-specific induction of Notch ligands, IL-22 was only up-regulated in acute inflammation, but rapidly down-regulated during regeneration. This implies that for targeting injury responses, e.g. via IL-22, species-specific differences, injury type and time points have to be considered. PMID: 29054964
    15. Suggest that IL-22 has various roles in tuberculosis immune responses. In particular, membrane-bound IL-22+ T cells may play important roles in the human immune response to Mycobacterium. PMID: 29050778
    16. this study shows immunoregulatory function of lacrimal glands -derived IL-22 in inhibiting IL-17-mediated ocular surface epitheliopathy in dry eye disease PMID: 28051088
    17. Serum IL-9 and IL-22 are associated with eosinophilia in cow's milk allergy, and decrease in these two cytokines with occurs with cow's milk elimination. PMID: 28934137
    18. Tracheal aspirates of patients infected by P. aeruginosa contain protease IV activity which further results in IL-22 degradation. This so far undescribed cleavage of IL-22 by a bacterial protease is likely to be an immune-evasion strategy that contributes to P. aeruginosa-triggered respiratory infections PMID: 27792459
    19. this study shows that copy number variations of the IL-22 gene are associated with ankylosing spondylitis in Chinese Han population PMID: 28716697
    20. IL-22 contributed to the inhibition of pulmonary microvascular endothelial cells apoptosis mediated by AngII through activating the JAK2/STAT3 signaling pathway. PMID: 28827891
    21. Findings provide a new perspective on the pro-inflammatory cytokine interleukin-22 in promoting aerobic glycolysis associated with tumor progression in human colon cancer cells. PMID: 28445985
    22. finding demonstrated that IL-22 could exert favorable effects on Diabetic nephropathy (DN) via simultaneously alleviating systemic metabolic syndrome and downregulating renal NLRP3/caspase-1/IL-1beta pathway, suggesting that IL-22 might have therapeutic potential for the treatment of DN. PMID: 28726774
    23. Our findings suggested that the IL-22 -429C/T gene polymorphisms might be associated with colon cancer. PMID: 28624523
    24. Authors found that SOCS3 and SOCS1 expression was reduced in vivo, in tumor lesions of BCC and SCC, as compared to other skin inflammatory conditions such as psoriasis, despite the high number of IL-22-secreting TILs. PMID: 28445952
    25. miR-330 inhibits IL-22-induced proliferation of HaCaT and HKC cell by targeting CTNNB1. PMID: 28501007
    26. IL-22 protects against sodium nitroprusside-induced apoptosis in rheumatoid arthritis-fibroblast-like synoviocytes by activating the STAT3 pathway and the downstream target gene, Bcl-2. PMID: 27493089
    27. IL22 plays a critical role in maintaining barrier homeostasis against intestinal pathogens and commensal bacteria, and, as a member of the IL10 superfamily, is critically involved in inflammation [Review] PMID: 29037907
    28. TARC production in HaCaT keratinocytes through the interaction between IL-22 and IL-22Ralpha facilitates T-cell migration in atopic dermatitis caused by house dust mites. PMID: 26914146
    29. IL-22 was found to be higher in PP than in PPMS (p<0.05). PMID: 27344023
    30. IL-22 is increased in patients with GCA and affects viability and gene expression of arterial cells, supporting a potential role in disease pathogenesis. PMID: 28968695
    31. suggest that the human IL-22BP isoforms have distinct spatial and temporal roles and coordinately fine-tune IL-22-dependent STAT3 responses in tissues as a type of rheostat. PMID: 27678220
    32. IL-18 cooperates with IL-15 to promote group 3 innate lymphoid cell (ILC3) proliferation and IL-22 production; describe an IL-18-induced, NF-kappaB-mediated mechanism that regulates IL-22 in ILC3s; at steady-state, IL-18 produced by dendritic cells mediates IL-22 production by ILC3s to help maintain normal tissue integrity PMID: 28842466
    33. results reveal that IL-22 increases intestinal epithelial permeability by upregulating Claudin-2 expression through the JAK/STAT pathway PMID: 28939759
    34. High serum levels of IL-22 were positively rather than inversely associated with several cardiometabolic risk factors. However, these associations did not translate into an increased risk for type 2 diabetes. PMID: 28143481
    35. mucosal-associated invariant T cells in the genital mucosa have a distinct IL-17/IL-22 profile and may have an important role in the immunological homeostasis and control of microbes at this site PMID: 27049062
    36. Results show that disease lavage IL-22 concentrations are highest in patients with pneumonia and lung cancer and does not significantly correlate with systemic inflammation. PMID: 27388918
    37. increased expression of IL-22Ralpha therefore promotes keratinocyte proliferation and pro-inflammatory cytokine production during UVB-induced skin inflammation, suggesting that UVB facilitates skin inflammation by increasing the responsiveness of keratinocytes to IL-22 PMID: 28558005
    38. In conclusion, Notch siganling appears to be an important mediator of the liver inflammation by modulating hepatic IL-22-secreting NKp46(+) innate lymphoid cells. PMID: 27800305
    39. High levels of IL-22 is associated with lyme disease. PMID: 27101991
    40. IL-23 released by keratinocytes in response to endogenous TLR4 ligands causes skin DCs, which selectively express IL-23R, to up-regulate their endogenous IL-23 production and drive an IL-22 response in naive CD4(+) T cells that mediates epidermal thickening. PMID: 27551155
    41. patients with T2DM and CAD exhibit increased serum IL-22 levels. Elevated serum IL-22 is associated with the incidence of CAD and T2DM. However, further in vitro study established the protective role of IL-22 against endothelial dysfunction, an essential process involved in the early development of atherosclerosis and vascular complications in T2DM. IL-22 might exert different functions under different context. PMID: 27829708
    42. plasma levels highly elevated after major liver resection PMID: 26853442
    43. IL-22 and its receptor have a crucial role in the development and pathogenesis of uveitis by facilitating inflammatory cell infiltration. PMID: 27166675
    44. there was no association between IL-22 SNPs (rs2227485, rs2272478, rs2227491) and the development of ulcerative colitis in a Mexican population PMID: 26994530
    45. The mutual benefit gained from interactions between the host and commensal intestinal bacteria-derived factors is an expanding field of research beginning to affect clinical practice. Data presented herein propose a supportive and fine-tuning role for butyrate in IL-22 signaling that might be therapeutically exploited by local butyrate administration PMID: 27801948
    46. this paper shows that human gammadelta T cell-antigen-presenting cells stimulate CD4+ T cell responses distinct from those induced by myeloid antigen-presenting cells to promote local barrier defense via mucosal release of IL-22 and calprotectin PMID: 28330898
    47. There was a significant direct correlation between levels of IL-10 and IL-22 in patients group (p=0.0005). The clinical severity of psoriasis was significantly correlated with high levels of IL-22 (p<0.0001). PMID: 27999243
    48. The levels of IL-22 mRNA in middle ear effusion of otitis media with effusion patients was higher in those with sinusitis than without. IL-22 expression was significantly higher in mucoid and purulent middle ear fluid samples than in serous fluid samples. PMID: 27729129
    49. IL-22 plays pathological roles in the development of recurrent hepatitis C after liver transplantation. PMID: 27123854
    50. The results suggest that IL-22 production in blood might act as a pathogenic factor in HIV infection. PMID: 25556046

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 14900

    OMIM: 605330

    KEGG: hsa:50616

    STRING: 9606.ENSP00000329384

    UniGene: Hs.287369