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  4. Human Interleukin 25,IL-25 ELISA kit

Human Interleukin 25,IL-25 ELISA kit

  • 中文名稱：
    人白介素25(IL-25)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11715h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人白介素25(IL-25)酶聯(lián)免疫試劑盒（CSB-E11715h）為雙抗夾心法ELISA試劑盒，定量檢測血清、cellculturesupernates、尿液、組織勻漿樣本中的IL25含量。IL25是一個值得關注的靶點。它屬IL-17細胞因子家族成員，參與多種生理病理過程。研究機制上，IL25能激活相關信號通路，調節(jié)免疫細胞功能，在哮喘、過敏性疾病等免疫相關病癥的發(fā)生發(fā)展中發(fā)揮作用。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于基礎醫(yī)學研究中炎癥反應機制探索、免疫調控模型構建以及疾病生物標志物篩選等場景，例如通過血清樣本分析體內IL - 25動態(tài)變化，或結合細胞培養(yǎng)實驗評估體外分泌水平，為科研人員開展免疫相關疾病機制研究提供標準化檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    IL 17E ELISA Kit; IL 25 ELISA Kit; IL-17E ELISA Kit; IL-25 ELISA Kit; IL17e ELISA Kit; IL25 ELISA Kit; IL25_HUMAN ELISA Kit; Interleukin 17E ELISA Kit; Interleukin 25 ELISA Kit; Interleukin-17E ELISA Kit; Interleukin-25 ELISA Kit; Interleukin17E ELISA Kit; Interleukin25 ELISA Kit; OTTHUMP00000027946 ELISA Kit; OTTHUMP00000246238 ELISA Kit; UNQ3120/PRO10272 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, cell culturesupernates, urine, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %87-98
    1:2Average %97
    Range %90-103
    1:4Average %97
    Range %90-106
    1:8Average %98
    Range %94-103
  • 回收率：
    The recovery of human IL-25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8480-89
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.892 2.752 2.822 2.711
    20002.299 2.179 2.239 2.128
    10001.645 1.599 1.622 1.511
    5000.982 1.002 0.992 0.881
    2500.561 0.602 0.582 0.471
    1250.350 0.366 0.358 0.247
    62.50.268 0.275 0.272 0.161
    00.109 0.112 0.111 　
  • 數(shù)據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
  • 基因功能參考文獻：
    1. this review details the structural characteristics, expression patterns, responder cells, biological properties, and role of IL-25 in disease pathogenesis PMID: 30345318
    2. this study shows that IL-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells PMID: 29966691
    3. Endometrial stromal cells differentiate into decidual stromal cells in the presence of ovarian hormones, resulting in the upregulation of IL25/IL17RB expression in endometrial stromal cells. PMID: 29257317
    4. In conclusion, the findings of the present study suggest that IL-25 but not IL-33 might contribute to the pathogenesis of chronic eosinophilic inflammation of the lung in patients with CEP. PMID: 28875265
    5. Nasal Il-25 is overexpressed in patients with chronic rhinosinusitis with nasal polyps and airway hypersensitiveness. PMID: 28870448
    6. IL-25-induced activation of nasal fibroblast and its association with the remodeling of chronic rhinosinusitis with nasal polyposis PMID: 28771607
    7. The authors concluded that IL-25 provides protection from amebiasis, which is dependent upon intestinal eosinophils and suppression of TNF-alpha. PMID: 28246365
    8. Inhibition of IL-25 resulted in decreased type 2 T cells and macrophages in the primary tumor microenvironments, both reported to enhance breast tumor invasion and subsequent metastasis to the lung. PMID: 27909985
    9. Our findings suggest that the IL-25/IL-25R axis may play an important role in promoting the recruitment and proinflammatory function of eosinophils in allergic asthma. PMID: 27685606
    10. Chronic rhinosinusitis with nasal polyps (CRSwNP) epithelial cells release TSLP and IL-25 when stimulated by poly(I:C) but not by DP or AF, suggesting that viral infection may contribute to maintain and amplify the T2 immune response seen in CRSwNP. PMID: 28127565
    11. Increased induction and expression of TSLP, IL-25, and IL-33 from nasal epithelial cells contribute to the pathophysiology of eosinophilic chronic rhinosinusitis. PMID: 26540312
    12. Women with endometriosis showed significantly higher levels of IL-25 in their PF (p=0.019) compared to controls. IL-25 levels did not correlate with the stage of endometriosis. PMID: 26852387
    13. IL-8 and IL-25 are targets of miR-20b. PMID: 26845056
    14. Our findings indicate that the alarmin cytokines IL-33 and IL-25 increase basophil activation and migratory potential, and may pose as a novel therapeutic targets for the treatment of allergic asthma. PMID: 26762527
    15. The IL17E rs79877597 SNP was a modifier of the risk for psoriasis disease severity and psoriatic arthritis. PMID: 26347322
    16. Findings indicate that the release of interleukin 25 (IL-25) from tumour-associated fibroblasts (TAFs) may serve as a check point for control of mammary tumour metastasis. PMID: 27089063
    17. IL-25 upregulated PD-L1 surface expression through the signaling pathways of JNK and STAT3, with STAT3 found to constitutively occupy the proximal region of the PD-L1 promoter. PMID: 26321145
    18. we have demonstrated that the expression of ET-1 and IL-25 was coordinately upregulated in the lesional keratinocytes of patients with AD and a murine AD model. PMID: 25903653
    19. IL-25 overexpression was observed in eosinophilic chronic rhinosinusitis and may serve as a mediator of eosinophilic CRS. PMID: 25975248
    20. expression of immunoreactivity for IL-17A, IL-17RA, IL-17E, and IL-17F was significantly elevated in prostatic tissue from benign prostatic hyperplasia and prostate cancer compared with that in controls PMID: 26356122
    21. This article focuses on evidence for the role of IL-25, IL-33 and TSLP in human allergic disease; although this triad of cytokines is described as epithelial derived, it is clear that their expression within lung tissue is much more widespread.[Review] PMID: 25705788
    22. The levels of mRNA for IL-25 were not significantly elevated in parotid gland tissues from Kimura Disease patients as compared to controls. PMID: 25676453
    23. asthmatic epithelial cells have an increased intrinsic capacity for expression of a pro-type 2 cytokine in response to a viral infection, and IL-25 is a key mediator of RV-induced exacerbations of pulmonary inflammation PMID: 25273095
    24. increased levels in patients with chronic rhinosinusitis with nasal polyps PMID: 25704964
    25. Because IL-25 has a major role in triggering type 2 responses, bronchial epithelial IL-25 expression is likely a key determinant of type 2 response activation in asthma PMID: 25133876
    26. Human chorionic gonadotropin up-regulates Il25, promoting the proliferation of decidual stromal cells by activation of JNK and AKT signal pathways. PMID: 24746746
    27. Patients with the 'IL-5, IL-17A, IL-25-high' airway inflammatory pattern are often uncontrolled asthmatics, despite daily treatment. PMID: 23957336
    28. IL-25 and type 2 innate lymphoid cells have roles in development of pulmonary fibrosis PMID: 24344271
    29. IL-25 expression is markedly reduced during human and experimental fulminant hepatitis. PMID: 23564603
    30. IL-25 and IL-33-driven type-2 innate lymphoid cells have roles in atopic dermatitis PMID: 24323357
    31. IL-25 enhances herpes simplex virus (HSV)-1 and vaccinia virus replication by inhibiting filaggrin expression, and IL-25 acts synergistically with IL-4 and IL-13 to enhance HSV-1 replication in vitro PMID: 23657503
    32. Suggest that IL-25 production by airway epithelial cells is regulated by the transcription and protein release levels and that allergen proteases likely play pivotal roles in both biological processes. PMID: 23590308
    33. Our data demonstrate that IL-33 plays a critical role in the rapid induction of airway contraction by stimulating the prompt expansion of IL-13-producing type 2 innate lymphoid cells, whereas IL-25-induced responses are slower and less potent. PMID: 23810766
    34. IL-25 Is Decreased in Sera of Patients With inflammatory bowel disease. PMID: 23429464
    35. IL-25 secreted from epithelial cells has the potential to promote airway remodeling in asthma. PMID: 22691357
    36. We observed significant downregulation of IL-25 in women with recurrent miscarriage compared with controls. PMID: 22266274
    37. IL-25 production is differently regulated by TNF-alpha and TGF-beta1 in the human g PMID: 20944558
    38. production is linked to the amount of specific IgE antibodies in blood samples of 6 yr old children in Germany PMID: 20492545
    39. Findings suggest that IL-25 is elevated in asthma and contributes to angiogenesis. PMID: 21205894
    40. IL-25 may have a role in atopic dermatitis PMID: 20861853
    41. Eosinophils are the main source of IL-25, whereas activated CD4+ memory T cells are the IL-17RB-expressing cells in Churg-Strauss syndrome. PMID: 20729468
    42. IL-17A and IL-25 had opposing effects on thymic stromal lymphopoietin regulation in human nasal epithelial cells PMID: 19968632
    43. Overexpression of IL-17E up-regulates gene expression of Th2 cytokines and induces growth retardation, jaundice, and multiorgan inflammation in a transgenic mouse model. PMID: 11714825
    44. Transgenic overexpression from humans into mice results in eosinophilia, B-lymphocyte hyperplasia, and altered antibody production. PMID: 12239140
    45. IL-17E may contribute to the induction and maintenance of eosinophilic inflammation by acting on lung fibroblasts. This supports a role for IL-17E in asthma pathophysiology. PMID: 16522458
    46. IL-25 acts by amplifying TH2 cell-mediated allergic airway inflammation. IL-25 itself does not significantly induce allergic inflammation in vivo. PMID: 16950278
    47. IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of TSLP-DC activated adaptive Th2 memory cells PMID: 17635955
    48. The upregulation of costimulation-induced IL-25 receptors and release of cytokines and chemokines from IL-25 treated costimulated Th cells were differentially regulated by intracellular JNK, p38 MAPK and NF-kappaB activity. PMID: 17719653
    49. Blocking IL-25 in an experimental model of allergic asthma prevents airway hyperresponsiveness, a critical feature of clinical asthma. PMID: 17889290
    50. Higher expressions of IL-17E, IL-17A, IL-17BR and IL-17R are associated with oral inflammation. PMID: 19095584

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-17 family
  • 組織特異性：
    Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.
  • 數(shù)據庫鏈接：

    HGNC: 13765

    OMIM: 605658

    KEGG: hsa:64806

    STRING: 9606.ENSP00000328111

    UniGene: Hs.302036