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中文名稱:人白介素31(IL-31)酶聯(lián)免疫試劑盒
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貨號:CSB-E16999h
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人白介素31(IL-31)酶聯(lián)免疫試劑盒(CSB-E16999h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的IL31含量。IL31是一個受關注的靶點。它是一種細胞因子,與皮膚瘙癢及炎癥反應密切相關。研究機制主要聚焦在其與受體結合后激活下游信號通路,引發(fā)免疫反應和神經(jīng)傳導改變,目前圍繞它在皮膚病治療等領域開展諸多研究。試劑盒檢測范圍為125 pg/ml-8000 pg/ml.,靈敏度為31.25 pg/ml.。適用于體外研究中對IL-31表達水平的動態(tài)監(jiān)測,如炎癥性疾病機制探索、免疫調節(jié)藥物開發(fā)或皮膚病理模型評估等科研場景。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:IL 31 ELISA Kit; IL-31 ELISA Kit; IL31 ELISA Kit; IL31_HUMAN ELISA Kit; Interleukin 31 ELISA Kit; Interleukin-31 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates.
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檢測范圍:125 pg/ml-8000 pg/ml.
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靈敏度:31.25 pg/ml.
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-31 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
88
Range %
84-96
1:2
Average %
96
Range %
90-102
1:4
Average %
84
Range %
81-87
1:8
Average %
88
Range %
83-94
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回收率:
The recovery of human IL-31 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
101
96-105
EDTA plasma (n=4)
107
100-114
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標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
8000
1.774
1.811
1.793
1.627
4000
1.003
1.055
1.029
0.863
2000
0.666
0.687
0.677
0.511
1000
0.401
0.412
0.407
0.241
500
0.289
0.302
0.296
0.130
250
0.245
0.255
0.250
0.084
125
0.211
0.216
0.214
0.048
0
0.163
0.168
0.166
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- An Investigative Study of Smoking Effects on IL-31 Levels and Leukocyte differential counts in Humans SJ Al-Ali,atmph-specialissues,2020
相關產(chǎn)品
靶點詳情
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功能:Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. May function in skin immunity. Enhances myeloid progenitor cell survival in vitro. Induces RETNLA and serum amyloid A protein expression in macrophages.
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基因功能參考文獻:
- Eosinophils are a major source of IL-31 in bullous pemphigoid and this cytokine may contribute to itch in these patients. PMID: 29693698
- Lung cancer patients homozygous CC for rs7977932 or carried the G allele of rs4758680 had significantly poorer prognoses compared to those who did not have these genotype; the rs7977932 CC genotype was significantly associated with metastasis and poor survival status in lung adenocarcinoma PMID: 29791232
- The findings suggest that rs7977932 and rs4758680 of IL-31 may be associated with the development and progression of the epithelial ovarian cancer in the Chinese population. PMID: 29484036
- this study shows that decreased serum levels of IL-31 correlate in treated patients with Relapsing-Remitting Multiple Sclerosis PMID: 29050818
- review: in-depth overview of the role of IL-31 in chronic skin diseases and the possible diagnostic and therapeutic applications in the management of these diseases PMID: 29046191
- Obtained results, as well as literature data, show that lower concentration of IL-31 in patients' serum may be correlated with its role in JAK/STAT signaling pathway which is involved in development of autoimmune blistering disease PMID: 28808661
- IL-31 gene polymorphisms were tightly associated with dilated cardiomyopathy susceptibility and contributed to worse prognosis in DCM patients. PMID: 28572699
- This study indicated that CXCL13 rather than IL-31 may have clinical values of diagnosis and prognosis in hepatocellular carcinoma. PMID: 27663978
- The first demonstration of the involvement of the IL-31/IL-31R axis in cancer came from studies in patients with mycosis fungoides/Sezary syndrome, the most frequent, cutaneous T cell lymphoma. Tumor cells were shown to produce IL-31, whose serum levels correlated with pruritus intensity. PMID: 28408397
- IL31 and its receptor, IL31RA, are highly expressed in various human and mouse cancer cell lines, as well as in tumor specimens from cancer patients. MC38 murine colon carcinoma cells depleted of IL31 exhibit an increase in invasive and migratory properties in vitro. PMID: 28147314
- this study shows that IL-31 may be involved in the pathogenesis of asthma and rhinitis; dust mite and mugwort allergy could increase it significantly PMID: 28303765
- While serum TSLP levels were unaffected by concomitant allergies and atopic comorbidities, serum levels of IL-31, IL-33 and sST2 were affected to a small extent. We found a positive correlation between TSLP, IL-31 and IL-33, and an inverse relationship between IL-33 and sST2 PMID: 27152943
- Suggest that IL-31 gene may play a role in the development of systemic lupus erythematosus. PMID: 26769434
- High IL31 expression is associated with Endometrial Cancer. PMID: 27340318
- In a CML patient with pruritus receiving imatinib mesylate, IL-31 and IL-33 serum levels were significantly higher than controls. Imatinib could cause keratinocyte injury, the release of IL-33, and the consequent interaction with its receptor on mast cells, inducing IL-31 secretion. The IL-31/IL-33 axis may be involved in the pathogenesis of skin side effects related to imatinib mesylate treatment. PMID: 26316486
- Distinct polymorphic variants of the IL-31 gene may be involved in the patho-genesis of mastocytosis, and IL-31 may be involved in the induction of pruritus in patients with mastocytosis. PMID: 27276346
- Serum/pleural fluid IL-31 levels are elevated in tuberculous pleural effusion. PMID: 26864868
- both SCF and IL-31 play an important role in mediating inflammation and enhancing severity of atopic asthma. PMID: 26853551
- IL-31 affects keratinocyte differentiation in multiple ways and the IL-1 cytokine network is a major downstream effector of IL-31 signaling in deregulating the physical skin barrier. PMID: 26944931
- Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology. PMID: 26198770
- The results of our analyses regarding serum levels and receptor expression do not suggest a central role of IL-31 in Mycosis fungoides/Sezary syndrome pathogenesis. PMID: 25488078
- In summary, TGF-beta1 and IL-31 were linked to progression from chronic hepatitis B to liver cirrhosis, and correlated well with the severity of disease. PMID: 25710085
- An increase of IL-31 serum levels in postmenopausal women with decreased bone mineral density, is shown. PMID: 26449657
- a possible systemic involvement of IL-31 and the absence of a systemic involvement of IL-33 in allergic contact dermatitis. PMID: 26357000
- crucial requirements for IL-31 signaling and its counter-regulation by SOCS3 PMID: 26306032
- IL-31 expression in allergic rhinitis aggravated and amplified Th2 inflammation as well as mucin production PMID: 25285475
- The IL-31 serum level was significantly higher in cutaneous T-cell lymphoma patients than in the control group but there were no positive correlation between IL-31 serum level and pruritus. PMID: 25176053
- IL-31 is overexpressed in the lesional skin of LP but its expression does not correlate with intensity of pruritus. PMID: 25756056
- IL-31 was increased after IVIG treatment in patients with KD and was significantly associated with CAL formation. PMID: 25122210
- Case Report: sporadic lichen amyloidosis with high expression of Il31. PMID: 24573820
- The results of this study suggest that the severity of atopic dermatitis in a Polish population is associated with some specific haplotypes of the IL-31 gene and renew questions concerning the role of IL-31 in pruritus in atopic dermatitis. PMID: 25534405
- enhanced Th2 cytokine levels was correlated with IL-31 expression in NPs and provide a possible explanation for IL-31's regulatory role in the pathogenesis of NPs. PMID: 24515918
- the results suggest that IL-31 may be a candidate gene associated with the pathogenesis of rheumatoid arthritis PMID: 25572240
- The interleukin IL-31/IL-31receptor axis contributes to tumor growth in human follicular lymphoma. PMID: 25283844
- TARC and interleukin-31 may be biomarkers for adult atopic dermatitis PMID: 24984931
- IL-31 may play an important role in the pathophysiology of uremic pruritus. PMID: 25270263
- IL-31-positive cells were observed as mononuclear infiltrating cells and as CD11b co-expressing cells in severe atopic dermatitis samples. PMID: 24667097
- Data show that STAT6 and NF-kappaB are central players in mediating IL-31 expression induced by IL-4/IL-33. PMID: 24943220
- EGFR-TK inhibitors could cause keratinocytes injury, the release of IL-33 and the consequent interaction with its receptor on mast cells, that induces the secretion of several factors capable to cause skin manifestations, included IL-31 PMID: 23794184
- IL-31 seems to be another sweat stimulator. PMID: 23874436
- CD4+CD26- malignant cells specifically produce IL-31 and clinical resolution of pruritus correlates with decreased IL-31 levels in the circulation PMID: 23698099
- IL-31 and IL-31RA are upregulated in patients with allergic rhinitis, and induce MUC5AC gene expression in human airway epithelial cells. PMID: 23392388
- IL-31 induces pro-inflammatory effects in activated human macrophages via STAT-1 and 5 phosphorylation. Interleukin-31-induced ERK 1/2 activation contributes to the underlying mechanism of Th1 cytokine IL-12 suppression in macrophages. PMID: 23621408
- Il-31 is not a TH2 cytokine in the classical sense but is likely to be expressed by a number of cells in an allergic situation in which IL-4 is present and possibly contribute to the allergic reaction. PMID: 23694808
- hypothesize that an altered regulation of IL-31 gene expression influence clinical course of AD. The available published data and our results lend support to an important role of IL-31 gene polymorphism in AD pathogenesis PMID: 22827739
- IL-31-631 T>G polymorphism was significantly associated with IL-31 blood level but not with disease susceptibility. PMID: 21952721
- T cells in chronic atopic dermatitis (AD) skin produce IL-31 and AD lesions contain increased levels of these IL-31-producing T cells. PMID: 22621183
- IL-31 serum levels correlate with the SCORAD score, sleeplessness, and Th2 cytokines including IL-4 and IL-13, in sixty children with the extrinsic type of atopic dermatitis. PMID: 22509760
- IL-31-specific activation of dendritic cells may be part of a positive feedback loop driving the progression of inflammatory skin diseases. PMID: 22539792
- Distinct SPINK5 and IL-31 gene variants (SNPs) were associated with the development of atopic eczema and non-atopic hand dermatitis in Taiwanese nurses. PMID: 22017185
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亞細胞定位:Secreted.
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組織特異性:Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
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