Human Interleukin-36 gamma(IL1F9) ELISA kit
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中文名稱:人白介素-36γ(IL1F9)酶聯(lián)免疫試劑盒
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貨號:CSB-EL011620HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人白介素-36γ(IL1F9)酶聯(lián)免疫試劑盒(CSB-EL011620HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL1F9含量。IL1F9是重要靶點(diǎn)。它屬于IL-1家族,在免疫調(diào)節(jié)等生理過程中扮演角色。研究機(jī)制方面,圍繞其與相關(guān)信號通路的相互作用展開,探索其在疾病發(fā)生發(fā)展中的功能,有望為免疫相關(guān)疾病的治療提供新方向。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,本試劑盒可廣泛應(yīng)用于體外實驗中探究IL - 36γ在免疫調(diào)節(jié)、炎癥機(jī)制中的功能,支持科研人員分析不同處理條件下(如藥物干預(yù)或基因修飾)細(xì)胞因子分泌動態(tài),或評估疾病模型中IL - 36γ的表達(dá)變化。其兼容多種生物樣本的特性,為體外研究提供了從基礎(chǔ)細(xì)胞實驗到組織水平檢測的完整解決方案,特別適用于炎癥相關(guān)信號通路研究、生物標(biāo)志物篩選及藥物作用機(jī)制探索等科研領(lǐng)域。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:IL 1 epsilon ELISA Kit; IL 1 related protein 2 ELISA Kit; IL 1(EPSILON) ELISA Kit; IL 1F9 ELISA Kit; IL 1H1 ELISA Kit; IL 1RP2 ELISA Kit; IL-1 epsilon ELISA Kit; IL-1-related protein 2 ELISA Kit; IL-1F9 ELISA Kit; IL-1H1 ELISA Kit; IL-1RP2 ELISA Kit; IL1E ELISA Kit; Il1f9 ELISA Kit; IL1F9_HUMAN ELISA Kit; IL1H1 ELISA Kit; IL1RP2 ELISA Kit; IL36 gamma ELISA Kit; IL36G ELISA Kit; Interleukin 1 epsilon ELISA Kit; Interleukin 1 family member 9 ELISA Kit; Interleukin 1 homolog 1 ELISA Kit; Interleukin 1 related protein 2 ELISA Kit; Interleukin 36 gamma ELISA Kit; Interleukin-1 epsilon ELISA Kit; Interleukin-1 family member 9 ELISA Kit; Interleukin-1 homolog 1 ELISA Kit
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縮寫:IL1F9
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:78 pg/mL-5000 pg/mL
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靈敏度:19.5 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IL1F9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 82-95 1:2 Average % 96 Range % 90-101 1:4 Average % 97 Range % 92-106 1:8 Average % 95 Range % 85-99 -
回收率:
The recovery of human IL1F9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 86-98 EDTA plasma (n=4) 92 88-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 5000 2.052 1.952 2.002 1.898 2500 1.611 1.497 1.554 1.450 1250 1.242 1.204 1.223 1.119 625 0.814 0.757 0.786 0.682 312 0.583 0.538 0.561 0.457 156 0.313 0.289 0.301 0.197 78 0.201 0.199 0.200 0.096 0 0.107 0.101 0.104 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Elevated serum IL-36γ levels in patients with ankylosing spondylitis and its association with disease activity S Shi,Molecular and cellular biochemistry,2023
- Different expression levels of interleukin-36 in asthma phenotypes J Li,/,2024
- The diagnostic value of Interleukin-36 cytokines in pleural effusions of varying etiologies X Song,Clinica chimica acta; international journal of clinical chemistry,2023
- Serum IL-36 cytokines levels in idiopathic pulmonary fibrosis and connective tissue disease-associated interstitial lung diseases Q Zhang,Clinica chimica acta; international journal of clinical chemistry,2023
- Interleukin-36 receptor antagonist stimulation in vitro inhibits peripheral and lung-resident T cell response isolated from patients with ventilator-associated pneumonia AA Khaled,International immunopharmacology,2024
- A novel biomarker for pleural effusion diagnosis: Interleukin‐36γ in pleural fluid L Guo,Journal of Clinical Laboratory Analysis,2022
- Serum IL-36 cytokines levels in type 2 diabetes mellitus patients and their association with obesity, insulin resistance, and inflammation Y Li,Journal of Clinical Laboratory Analysis,2020
- Insight into innate immune response in “Yusho”: The impact of natural killer cell and regulatory T cell on inflammatory prone diathesis of Yusho patients Y Kamio,Environmental Research,2020
- Acitretin inhibits IL-17A-induced IL-36 expression in keratinocytes by down-regulating IκBζ Tu J,International Immunopharmacology,2019
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.
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基因功能參考文獻(xiàn):
- serum IL-36gamma levels were higher in active systemic lupus erythematosus patients and correlated with disease activity and arthritis PMID: 29571080
- Cathepsin S was identified as the major IL-36gamma-activating protease expressed in epithelial cells. PMID: 28289191
- enhanced expression of IL-36gamma was observed in plasma and bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome because of bacterial pneumonia PMID: 28176791
- IL-36-mediated IL-6 and CXCL8 production in human lung fibroblasts and bronchial epithelial cells may be involved in pulmonary inflammation especially caused by bacterial or viral infections. PMID: 28869889
- With a focus on the skin as a target for microbial and viral invasion, the current knowledge of IL-36: IL-36alpha, IL-36beta and IL-36gamma, functions is reviewed. One physiological function of the IL-36smay be to counteract microbial immune evasion. [Review] PMID: 28811383
- IL-36gamma inhibits differentiation and induces inflammation of keratinocyte via Wnt signaling pathway in psoriasis. PMID: 28924372
- IL-36gamma-stimulated endothelial cells secrete the proinflammatory chemokines IL-8, CCL2 and CCL20. PMID: 27673278
- skin injury increases IL-36gamma via the activation of TLR3-SLUG-VDR axis and IL-36gamma induces REG3A to promote wound healing PMID: 28774595
- Autocrine and Paracrine Regulation of Keratinocyte Proliferation through a Novel Nrf2-IL-36gamma Pathway PMID: 27183581
- Here the authors show that Mycobacterium tuberculosis infection of macrophages induces IL-36gamma production in a 2-stage-regulated fashion. PMID: 27389350
- IL-36gamma, a member of the IL-1 superfamily, is involved in host defense and contributes to proinflammatory responses and development of inflammatory diseases. PMID: 27853811
- IL-36gamma is significantly more strongly expressed in the epidermis of patients with psoriasis-based erythroderma than in other inflammatory skin diseases. PMID: 26524325
- Study shows that plasma concentrations of IL-36alpha and IL-36gamma are overexpressed in active systemic lupus erythematosus patients and that IL-36alpha has a substantial pro-inflammatory effect through regulation of IL-6 and CXCL8 production. PMID: 26516833
- IRF6 is likely to promote inflammation to P. gingivalis through its regulation of IL-36gamma. PMID: 26819203
- Findings indicate that Interleukin (IL)-1beta-induced interleukin 36 gamma (IL-36gamma) expression is mediated by the activation of transcriptional factors, NF-kappaB p65 and AP-1 (c-jun). PMID: 26562662
- IL36G was identified as strong regulators of skin pathology in both lesional and non-lesional skin samples. PMID: 25897967
- Decreased Langerhans cell responses to IL36G: altered innate immunity in patients with recurrent respiratory papillomatosis PMID: 24950037
- IL-36gamma expression inversely correlated with the progression of human melanoma and lung cancer. PMID: 26321222
- IL-36gamma is a valuable biomarker in psoriasis patients, both for diagnostic purposes and measurement of disease activity during the clinical course PMID: 25525775
- CAMP induces IL-36gamma expression leading to initiation of skin inflammation and occasional exacerbations of psoriasis. PMID: 25305315
- IL-36 promotes myeloid cell infiltration, activation, and inflammatory activity in skin PMID: 24829417
- This is the first report of extracellular release of endogenous IL-36gamma through pyroptosis suggesting a function of IL-36gamma as an alarmin. PMID: 22318382
- Data presented herein shed further light on involvement of T-bet in innate immunity and suggest that IL-36gamma, besides IFNgamma, may contribute to functions of this transcription factor in immunopathology. PMID: 23095752
- Interleukin-36 (IL-36) ligands require processing for full agonist (IL-36alpha, IL-36beta, and IL-36gamma) or antagonist (IL-36Ra) activity PMID: 21965679
- Regulation and function of the IL-1 family cytokine IL-1F9 in human bronchial epithelial cells. PMID: 20870894
- Expression of IL-1F9 is increased in human plaque psoriasis skin and is overexpressed in a transgenic mouse psoriasis model. PMID: 21242515
- IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells PMID: 14734551
- This is the first report of IL-1 genotype association with the inflammation of skeletal muscle following acute resistance exercise that may potentially affect the adaptations to chronic resistance exercise. PMID: 15331687
- This report demonstrates expression of IL1F9 by bronchial epithelial cells induced by pro-inflammatory stimuli, suggesting a function of this molecule in airway inflammation. PMID: 15701729
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亞細(xì)胞定位:Cytoplasm. Secreted.
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蛋白家族:IL-1 family
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組織特異性:Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. Expressed in bronchial epithelial. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional ps
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