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  4. Human Involucrin,iNV ELISA Kit

Human Involucrin,iNV ELISA Kit

  • 中文名稱：
    人套膜蛋白(iNV)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09332h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人套膜蛋白(iNV)酶聯(lián)免疫試劑盒（CSB-E09332h）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織培養(yǎng)上清液、組織勻漿樣本中的IVL含量。人套膜蛋白(iNV)是一種重要的細(xì)胞膜蛋白，其背景研究涉及其在細(xì)胞信號傳導(dǎo)和細(xì)胞結(jié)構(gòu)維持中的關(guān)鍵作用。研究機制主要關(guān)注其與細(xì)胞內(nèi)其他蛋白的相互作用，以及通過ELISA等檢測方法對其濃度進(jìn)行定量分析。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，可滿足科研場景中對病毒感染機制、抗病毒藥物篩選、疫苗研發(fā)等方向的研究需求，支持體外細(xì)胞模型、動物實驗樣本及病理組織樣本的定量檢測，為病毒學(xué)基礎(chǔ)研究及生物醫(yī)學(xué)應(yīng)用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    INVO_HUMAN ELISA Kit; Involucrin ELISA Kit; IVL ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, cell culture supernates, tissue homogenates
  • 檢測范圍：
    1.25 ng/mL-80 ng/mL
  • 靈敏度：
    0.31 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human iNV in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %102
    Range %95-109
    1:4Average %90
    Range %85-94
    1:8Average %84
    Range %80-88
  • 回收率：
    The recovery of human iNV spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.915 2.750 2.833 2.666
    402.210 2.132 2.171 2.004
    201.561 1.476 1.519 1.352
    100.951 0.997 0.974 0.807
    50.616 0.602 0.609 0.442
    2.50.396 0.427 0.412 0.245
    1.250.274 0.258 0.266 0.099
    00.162 0.171 0.167
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
  • 基因功能參考文獻(xiàn)：
    1. Together, these results suggest that GR modulates involucrin expression of keratinocytes by regulating the intracellular signaling network in a ligand-independent manner. PMID: 24515279
    2. over-expression of Nnat was evident along with involucrin and cytokeratin1 expression PMID: 24252747
    3. involucrin is up-regulated in psoriatic keratinocytes PMID: 23283814
    4. KLF4 is part of a multiprotein complex that interacts that the hINV promoter distal regulatory region to drive differentiation-dependent hINV gene expression in epidermis. PMID: 23599428
    5. Compared with the control, the level of expression of the IVL gene in NCTC 2544 cells was increased after 8 h of treatment with hyaluronic acid or Plantaricin A preparations. PMID: 22742591
    6. Therefore, together with trichohyalin, the cross-linking due to involucrin is also responsible for the mechanical resistance of the corneous trabeculae present among the empty spaces of the medulla of the human hair. PMID: 22197495
    7. The authors found that HPV 16 E6 but not E7 repressed IVL promoter activity in proliferating human foreskin keratinocyte cells. PMID: 22333115
    8. higher expression in the fetal skin than in adult skin PMID: 19701759
    9. expression of the epidermal differentiation complex genes filaggrin and involucrin was strongly decreased directly by IL-13. PMID: 20208004
    10. Transcriptional activation of the involucrin gene by calcium in keratinocytes. PMID: 9159190
    11. involucrin gene contains distinct regulatory elements that regulate expression during epidermal differentiation PMID: 11850802
    12. transactivation of involucrin by lens epithelium-derived growth factor (LEDGF) PMID: 12126624
    13. the AP-1 site and the vitamin D response element in the involucrin promoter play important roles in mediating the action of 1,25-dihydroxyvitamin D3 on involucrin expression PMID: 12445200
    14. p38 delta is the major p38 isoform driving suprabasal hINV gene expression PMID: 12810719
    15. role for AP1 transcription factors in the regulation of human corneal epithelial cell involucrin gene expression. PMID: 15037572
    16. human involucrin promoter activity is regulated by green tea polyphenol and curcumin via opposing effects on CCAAT/enhancer-binding protein function PMID: 15044435
    17. cascade regulates activator protein one, Sp1, and CCATT/enhancer-binding protein transcription factor DNA binding to two discrete involucrin promoter regions, the distal- and proximal-regulatory regions, to regulate involucrin gene expression. PMID: 15191537
    18. cholesterol depletion alters involucrin gene expression through activation of p38alpha/beta. PMID: 15304097
    19. YY-1 and c-Jun transcription factors participate in the repression of the human involucrin promoter. PMID: 15586248
    20. Involucrin expression of keratinocytes is suppressed by roxithromycin through direct inhibition of AP-1 and indirect inhibition of NF-kappaB. PMID: 16140218
    21. AP1, Sp1, and C/EBP transcription factors are required for appropriate differentiation-dependent involucrin expression. PMID: 16374477
    22. involucrin gene expression inhibition by apigenin is associated with a specific reduction in phosphorylation of protein kinase Cdelta Tyr311 PMID: 16982614
    23. Pyk2 regulation is associated with increased expression of Fra-1 and JunD, activator protein-1 transcription factors known to be required for involucrin expression. PMID: 17205062
    24. There is a major role for STAT5a in the differentiation of keratinocytes, where it contributes to involucrin expression by activating the PPARgamma signal. PMID: 17330131
    25. BAG-1L also potentiated transactivation of known vitamin-D-responsive gene promoters, osteocalcin and 24-hydroxylase, and enhanced VDR-dependent transcription and protein expression of the keratinocyte differentiation marker, involucrin. PMID: 17662274
    26. a large multiprotein complex, which includes Fra-1, p300, P/CAF, junD, junB, and Sp1 acts at the AP1-5 site to produce a synergistic increase in hINV gene expression PMID: 17882273
    27. These findings indicate that PKCdelta activity for activation of involucrin promoter requires Y(311) and a second tyrosine residue; however, any one of the several tyrosine residues can serve in the secondary role. PMID: 17943181
    28. PPAR gamma regulates involucrin expression by controlling the AP-1 signal and p38 activation in 1 alpha,25(OH)2D3-induced keratinocyte differentiation. PMID: 18077140
    29. The expression of loricrin and involucrin in invovled and uninvolved skin of patients with atopic dermatitis is reported. PMID: 18166499
    30. siRNA for PRKD1 resulted in reduction of mRNA levels of PKD1, altered cell phenotype and promotion of keratinocyte differentiation, demonstrated by increased expression of involucrin and keratin 10 mRNAs PMID: 18259765
    31. RT-PCR and western blot analysis revealed a delay in the expression of the differentiation markers K1, K10 and involucrin in HaCaT cells compared with normal human keratinocytes PMID: 18637039
    32. Infection by HPV may alter the differentiation status of the epidermis, leading to earlier involucrin expression PMID: 19515043

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  • 亞細(xì)胞定位：
    Cytoplasm. Note=Constituent of the scaffolding of the cornified envelope.
  • 蛋白家族：
    Involucrin family
  • 組織特異性：
    Keratinocytes of epidermis and other stratified squamous epithelia.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6187

    OMIM: 147360

    KEGG: hsa:3713

    STRING: 9606.ENSP00000357753

    UniGene: Hs.516439