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Human Isodesmosine (IDES) ELISA Kit

  • 中文名稱:
    人異鎖鏈素(IDES)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E14052h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人異鎖鏈素(IDES)酶聯(lián)免疫試劑盒(CSB-E14052h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、尿液、組織勻漿樣本中的IDES含量。試劑盒檢測范圍為47 pg/mL-3000 pg/mL,科研人員可利用探索IDES在器官纖維化模型中的表達特征,分析其在炎癥性疾病動物或細胞實驗中的動態(tài)變化,或通過檢測體液樣本評估其作為生物標志物的潛在價值,為疾病機制研究提供可靠工具。適用于體外研究場景。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    IDES
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, urine, tissue homogenates
  • 檢測范圍:
    47 pg/mL-3000 pg/mL
  • 靈敏度:
    11.75 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human IDES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %80-92
    1:2Average %95
    Range %90-99
    1:4Average %99
    Range %94-103
    1:8Average %86
    Range %82-92
  • 回收率:
    The recovery of human IDES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-105
    EDTA plasma (n=4)9994-104
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.291 2.287 2.289 2.156
    15001.752 1.757 1.755 1.622
    7501.091 1.114 1.103 0.970
    3750.675 0.698 0.687 0.554
    187.50.438 0.457 0.448 0.315
    93.750.297 0.282 0.290 0.157
    470.225 0.235 0.230 0.097
    00.133 0.132 0.133  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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