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中文名稱:人II型角蛋白,細胞支架1(KRT1)酶聯(lián)免疫試劑盒
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貨號:CSB-EL012503HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人II型角蛋白,細胞支架1(KRT1)酶聯(lián)免疫試劑盒(CSB-EL012503HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的KRT1含量。KRT1 即角蛋白 1,是上皮細胞內(nèi)中間絲網(wǎng)絡(luò)的重要組成部分。其表達異常與多種皮膚疾病相關(guān),如魚鱗病等。研究機制上,主要圍繞其在維持細胞結(jié)構(gòu)、信號傳導(dǎo)中的作用,通過影響細胞黏附、遷移等過程,來探究其在疾病發(fā)生發(fā)展中的影響。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于皮膚生物學(xué)研究、上皮細胞分化模型構(gòu)建、疾病相關(guān)分子機制探索等科研場景,為研究KRT1在組織修復(fù)、腫瘤微環(huán)境調(diào)控等領(lǐng)域的功能提供了可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:67 kDa cytokeratin ELISA Kit; CK-1 ELISA Kit; CK1 ELISA Kit; Cytokeratin-1 ELISA Kit; Cytokeratin1 ELISA Kit; EHK ELISA Kit; EHK1 ELISA Kit; Epidermolytic hyperkeratosis 1 ELISA Kit; EPPK ELISA Kit; Hair alpha protein ELISA Kit; K1 ELISA Kit; K2C1_HUMAN ELISA Kit; Keratin ELISA Kit; Keratin type II cytoskeletal 1 ELISA Kit; Keratin-1 ELISA Kit; Keratin1 ELISA Kit; KRT 1 ELISA Kit; Krt1 ELISA Kit; KRT1A ELISA Kit; NEPPK ELISA Kit; type II cytoskeletal 1 ELISA Kit; Type II keratin Kb1 ELISA Kit; Type-II keratin Kb1 ELISA Kit
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縮寫:KRT1
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human KRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 87-93 1:2 Average % 85 Range % 81-90 1:4 Average % 99 Range % 95-104 1:8 Average % 97 Range % 91-103 -
回收率:
The recovery of human KRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 87 82-91 EDTA plasma (n=4) 92 85-96 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.862 2.898 2.880 2.727 5 1.991 1.999 1.995 1.842 2.5 1.172 1.198 1.185 1.032 1.25 0.640 0.674 0.657 0.504 0.625 0.425 0.436 0.431 0.278 0.312 0.353 0.368 0.361 0.208 0.156 0.260 0.268 0.264 0.111 0 0.152 0.154 0.153 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique Xu J.et al,Evid Based Complement Alternat Med. ,2017
相關(guān)產(chǎn)品
靶點詳情
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功能:May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
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基因功能參考文獻:
- The authors report a large Italian family affected Palmoplantar Keratoderma and Charcot Marie Tooth disease. Two different mutated genes, KRT1 and MPZ were responsible for the two main clinical signs. Exome analysis detected two missense mutations, one in KRT1 and one in MPZ. PMID: 27639257
- Results show that missense mutations exert dominant negative effects on the keratins K1/K10 protein structure by altering inter-chain interactions. PMID: 27421141
- KRT1 and the specific polymorphism of KRT1 in this Chinese Han population are associated with autoimmune diseases SLE and SSc PMID: 29028840
- Case Report: post-zygotic mosaicism of KRT/1o mutations in epidermolytic Ichthyosis. PMID: 27722766
- KRT1 played an important role of maintaining epithelial barrier and its down-regulation in intestinal tissue was correlated with the progression of inflammatory bowel disease. PMID: 28111259
- Report genetic/clinical spectrum of KRT1 mutations in keratinopathic ichthyosis. PMID: 26581228
- demonstrated the presence of a genetic cutaneous mosaicism. Both patients carry the KRT1 pI479T substitution, but in the palmoplantar areas of one of them, only the mutated allele is expressed (hemizygous). This leads to highlight a new type of cutaneous mosaic, the palmoplantar mosaicism PMID: 25904304
- In our study, the missense mutation in the proband and his mother with epidermolytic ichthyosis was a single amino acid difference in codon 478, which causes more serious skin manifestations PMID: 25808222
- Complete structure of an epithelial keratin 1/keratin 10 dimer has been presented. PMID: 26181054
- These findings indicate that exogenous FABP4 interacts with plasma membrane proteins, specifically CK1. PMID: 26343611
- analysis of a heterozygous novel splice junction mutation in the 2B domain of KRT1 in a family with diffuse palmoplantar keratoderma PMID: 25429721
- In ichthyosis with confetti, a causal de novo KRT1 mutation had a C-terminal frameshift, replacing 22 C-terminal AAs with an alternate 30-AA peptide. It distorted the IF network and mislocalized to the nucleus. Reversion occurred by mitotic recombination. PMID: 25774499
- In HeLa cells transiently expressing C2GnT-M-GFP, knockdown of KRT1 does not affect Golgi morphology but leaves C2GnT-M outside of the Golgi, resulting in the formation of sialyl-T antigen. PMID: 25605727
- Decreased levels of cytokeratin-1 is associated with breast cancer. PMID: 25073515
- Hsp74, a potential bladder cancer marker, has direct interaction with keratin 1. PMID: 25050384
- identified among endothelial antigens to which antibodies are produced during heart transplant rejection PMID: 23707440
- Case Report/Letter: specific mutation in 2B domain of KRT1 gives rise to mild phenotype of epidermolytic hyperkeratosis mimicking ichthyosis bullosa of Siemens. PMID: 23623204
- study reports 2 related women of Colombian origin, affected by a severe ichthyosis curth-macklin phenotype, who present a novel KRT1 mutation c.1577delG (p.Gly526Alafs*88) PMID: 22834809
- Absence of Krt1 caused a prenatal increase in interleukin-18 (IL-18) and the S100A8 and S100A9 proteins, accompanied by a barrier defect and perinatal lethality. PMID: 23132931
- Among Japanese patients with bullous congenital ichthyosiform erythroderma for which genetic diagnosis was determined, all showed mustations in KRT1 or KRT10. PMID: 23182068
- Identification Keratin 1 as a cDDP-resistant protein in nasopharyngeal carcinoma cell lines. PMID: 22348822
- High cytokeratin is associated with colorectal carcinogenesis. PMID: 21912905
- we describe one Chinese family affected with EHK, type PS-1 (severe palmoplantar hyperkeratosis, type 1) and report a recurrent missense mutation (c.1436T>C) in the 2B rod domain of KRT1 in this family. PMID: 22250628
- mutation analysis in patients with epidermolytic ichthyosis by direct sequencing of KRT1 and KRT10 genes; identified 14 different mutations, of which four have not been published previously PMID: 21271994
- keratin 1 L12 domain mutations are associated with a milder epidermolytic ichthyosis phenotype with pronounced palmoplantar keratoderma, and without neonatal erythroderma and scaling. PMID: 20500210
- Data demonstrate that genetic variants in the KRT1 interval contribute to quantifiable differences in the migration rates of keratinocytes isolated from different individuals. PMID: 17668073
- alpha-keratin intermediate filaments have a low-density core as seen by cryoelectron microscopy PMID: 12064938
- The humans hair Keratin 1 genes are each clustered in the genome and clusters are part of the large typeI epithelial keratin gene domains on chromosomes. PMID: 15797458
- bullous congenital ichthyosiform erythroderma (BCIE) caused by a mutation in the 1A helix initiation motif of keratin 1. PMID: 16361731
- A new genetic polymorphism has been detected, which is especially prevalent among the African-American population. PMID: 16417221
- Allelic expression differences result from the cumulative contribution of multiple DNA sequence polymorphisms. PMID: 16789827
- The 2 keratin 1 mutations are associated with tonotubular keratin, i.e. 'whorls' of aggregated keratin that form tubules as seen in transverse or in longitudinal sections PMID: 18795921
- Mutation L437P in the 2B domain of keratin 1 causes diffuse palmoplantar keratoderma in a Chinese pedigree. PMID: 19470048
- Keratin 1, an intermediate filament network component, is the binding partner of the lymphocytic choriomeningitis virus nucleoprotein. PMID: 19494018
- Infection by HPV may alter the differentiation status of the epidermis, leading to delayed or absent expression of cytokeratin 1. PMID: 19515043
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相關(guān)疾?。?/div>Epidermolytic hyperkeratosis (EHK); Ichthyosis hystrix, Curth-Macklin type (IHCM); Keratoderma, palmoplantar, non-epidermolytic (NEPPK); Ichthyosis annular epidermolytic (AEI); Keratoderma, palmoplantar, striate 3 (SPPK3)亞細胞定位:Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells.蛋白家族:Intermediate filament family組織特異性:The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.數(shù)據(jù)庫鏈接:
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