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Human Kininogen-1(KNG1) ELISA kit

  • 中文名稱:
    人激肽原1(KNG1)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL012479HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人激肽原1(KNG1)酶聯(lián)免疫試劑盒(CSB-EL012479HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的KNG1含量。KNG1是一個(gè)重要靶點(diǎn)。它參與血管生成、炎癥反應(yīng)等生理過程。在研究中,關(guān)注其如何調(diào)控血管新生、調(diào)節(jié)炎癥信號(hào)通路等機(jī)制。深入研究KNG1有助于了解相關(guān)疾病發(fā)病機(jī)制,為開發(fā)針對該靶點(diǎn)的治療藥物提供理論基礎(chǔ)。試劑盒檢測范圍為47 ng/mL-3000 ng/mL,適用于科研領(lǐng)域中對KNG1的定量分析,例如探究其在疾病模型中的動(dòng)態(tài)變化、評估藥物干預(yù)對激肽通路的影響,或作為相關(guān)分子機(jī)制研究的工具。可為體外實(shí)驗(yàn)提供可靠的蛋白濃度數(shù)據(jù)支持,助力于基礎(chǔ)醫(yī)學(xué)研究與生物標(biāo)志物篩選。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Alpha-2-thiol proteinase inhibitor ELISA Kit; BDK ELISA Kit; BK ELISA Kit; Bradykinin ELISA Kit; Bradykinin included ELISA Kit; Fitzgerald factor ELISA Kit; FLAUJEAC FACTOR ELISA Kit; High molecular weight kininogen ELISA Kit; HMWK ELISA Kit; Ile-Ser-Bradykinin ELISA Kit; Kallidin I ELISA Kit; Kallidin II ELISA Kit; KNG ELISA Kit; KNG1 ELISA Kit; KNG1_HUMAN ELISA Kit; Low molecular weight growth-promoting factor ELISA Kit; WILLIAMS FACTOR ELISA Kit; Williams-Fitzgerald-Flaujeac factor ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    47 ng/mL-3000 ng/mL
  • 靈敏度:
    11.75 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human KNG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:100 Average % 99
    Range % 90-103
    1:200 Average % 105
    Range % 98-109
    1:400 Average % 92
    Range % 83-97
    1:800 Average % 95
    Range % 87-98
  • 回收率:
    The recovery of human KNG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 108 99-112
    EDTA plasma (n=4) 94 86-98
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    3000 2.805 2.673 2.739 2.626
    1500 2.300 2.255 2.278 2.165
    750 1.518 1.482 1.500 1.387
    375 0.766 0.779 0.773 0.660
    187.5 0.396 0.414 0.405 0.292
    94 0.241 0.233 0.237 0.124
    47 0.152 0.158 0.155 0.042
    0 0.114 0.112 0.113  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • 基因功能參考文獻(xiàn):
    1. Kininogen binds to the cell surface-exposed proteins of Candida parapsilosis. PMID: 28651026
    2. Three loci showed robust, replicating association with circulating FXI levels: KNG1 (rs710446, P-value = 2.07 x 10-302), F11 (rs4253417, P-value = 2.86 x 10-193), and a novel association in GCKR (rs780094, P-value = 3.56 x10-09), here for the first time implicated in FXI regulation. The two first SNPs (rs710446 and rs4253417) also associated with partial thromboplastin time PMID: 28053049
    3. findings show that the KNG1 locus plays a central role as a genetic determinant of the entire coagulation pathway and thrombus/clot formation PMID: 28005926
    4. Urinary KNG1 and RBP4 clearly respond to AKI. Additionally, RBP4 could predict recovery by monitoring this protein levels over time after AKI, as RBP4 reflects patient's normalization earlier than sCr values do. PMID: 26792617
    5. KNG1 rs710446/ rs2304456 SNPs are not related to the incidence of Alzheimer's disease in a Hunan Han Chinese population. PMID: 26884824
    6. Low urine KNG1 expression is associated with clear cell renal cell carcinoma. PMID: 26420021
    7. The results of this study supported the association of the epistatic interactions of ALOX5AP, THBD, and KNG1 and present novel evidence for the main effect of KNG1 gene on IS susceptibility. PMID: 26159646
    8. Bradykinin stimulates pro-contractile signalling mechanisms in human myometrial cells and arrestin proteins play key roles in their regulation. PMID: 25766502
    9. Measuring plasma levels of cleaved HK may be a sensitive mean of assessing disease severity in HAE-C1-INH patients. PMID: 24552232
    10. The antimicrobial protein kininogen evolved adaptively in mammals and human populations. PMID: 23505046
    11. results demonstrate that HKa accelerates the onset of EPC senescence by stimulating JNK/FOXO4/MnSOD pathway, its effect is mediated by the HC PMID: 24984152
    12. When the concentrations of IGFBP-6 or KNG1 were greater than 98.5 pg/ml or 88.5 ng/ml, respectively, they predicted the proliferative vitreoretinopathy prognosis. PMID: 23808406
    13. uPAR mediates efferocytosis through high molecular weight kininogen interaction with phosphatidylserine on apoptotic cells PMID: 24688027
    14. these results suggest that D5(H) of high molecular weight kininogen may modulate cell adhesion and invasion together with actinin-4. PMID: 23000411
    15. KNG1 and F11 are the main genetic regulators of plasma FXI level. PMID: 22701019
    16. The results showed that bradykinin stimulates Toll-like receptor 2 transcription and translation by activation of protein kinase C as well as proto-oncogene protein AKT. PMID: 21982724
    17. kininogen-1 and thymosin-beta(4) are potential new biomarkers for human chronic hepatitis C. PMID: 21496200
    18. Bacteroides fragilis and Bacteroides thetaiotaomicron, were found to bind HK and fibrinogen PMID: 21527472
    19. High molecular weight kininogen activates B2 receptor signaling pathway in human vascular endothelial cells. PMID: 21586566
    20. Kininogen-1 and IGFBP-6 are expressed in serum and vitreous humor in proliferative vitreoretinopathy patients. PMID: 21054968
    21. Bradykinin, acting via bradykinin 2 receptors, initiates an important signal directing the invasion of glioma patients' cells toward blood vessels. PMID: 21451024
    22. KNG1 Ile581Thr and susceptibility to venous thrombosis. PMID: 21270443
    23. expression of ACE is lowest in elderly women, despite the presence of similar BK degradation in pericardial fluid PMID: 19592120
    24. These findings provide the first genome-wide significant evidence of association with plasma adiponectin at the CDH13 locus and identify a novel uncommon KNG1-ADIPOQ haplotype strongly associated with adiponectin levels in Filipinos. PMID: 20876611
    25. KNG1 SNP rs710448 (allele frequency 42%) was associated with decreased risk (OR 0.44, 95% CI 0.3-0.8) among women but not men. PMID: 19716087
    26. High-glucose stimulates tubular proinflammatory, profibrotic and angiogenic signals, which is partly mediated through bradykinin via MAPK signalling and partly through protein kinase C independent of bradykinin. PMID: 19923143
    27. Single Nucleotide Polymorphisms in KNG1 is associated with activated partial thromboplastin time. PMID: 20303064
    28. our findings suggest that bradykinin and bradykinin B2 receptors are involved in the modulation of mechanisms participating in inflammatory synovitis PMID: 20050188
    29. Bradykinin enhances the migration of chondrosarcoma cells by increasing alpha2beta1 integrin expression through the BK receptors/PLC/PKCdelta/NF-kappaB signal transduction pathway. PMID: 19885862
    30. High molecular weight kininogen (HKa) mimics lipopolysaccharides by triggering a paracrine pathway in monocytes that depends on TNF-alpha and IL-1beta. Upregulation of tissue factor in monocytes by cleaved HKa is dependent on TNF-alpha and IL-1beta. PMID: 19966052
    31. inhibition of vitronectin-mediated haptotaxis and haptoinvasion of MG-63 cells by domain 5 PMID: 11689005
    32. Heat shock protein 90 catalyzes activation of the prekallikrein-kininogen complex in the absence of factor XII. PMID: 11792853
    33. down-regulates cathepsin G-induced platelet activation by forming a complex with cathepsin G and thus prevents binding of cathepsin G to platelets PMID: 11920276
    34. a defined region can abolish platelet adhesion to vitronectin and block platelet aggregation PMID: 11970955
    35. Ferritin binds to light chain of human H-kininogen and inhibits kallikrein-mediated bradykinin release. PMID: 12071855
    36. kininogen has as role as a antithrombotic agent by inhibiting plasminogen activator inhibitor-1 PMID: 12082110
    37. Bradykinin induces IL-6 production in human airway smooth muscle cells. PMID: 12594059
    38. free HK, or HK in complex with prekallikrein but not in complex with factor XI, interacts with the endothelium and can maintain endothelial cell quiescence by preventing endothelial stimulation by thrombin. PMID: 12663668
    39. transcriptional activation of the interleukin-8 promoter by bradykinin involves the prostanoid-independent activation of nuclear factor-kappaB, and prostanoid-dependent activation of activating protein-1 and nuclear factor-interleukin-6 PMID: 12748173
    40. kininogen is a novel and direct target of farnesoid X receptor PMID: 12761213
    41. Bradykinin is a powerful spasmogen via B(2) receptor activation in the normal and, especially, in the inflamed human gallbladder. PMID: 12851878
    42. sequences in domain 5 of high molecular weight kininogen interact with heparin and zinc PMID: 12911595
    43. High molecular weight kininogen regulates platelet-leukocyte interactions by bridging Mac-1 and glycoprotein Ib PMID: 12952972
    44. His-Gly-Lys motif and lysine residue (Lys487) play essential roles in inhibition of cell adhesion and invasion in vitro and in prevention of metastasis of cancer cells in vivo PMID: 14506238
    45. COX-2 induction by bradykinin in human pulmonary artery smooth muscle cells is mediated by the cyclic AMP response element through a novel autocrine loop involving endogenous prostaglandin E2, EP2 receptor, and EP4 receptor PMID: 14517215
    46. binds with high specificity and affinity to microvascular endothelial cells, but with lower affinity to astrocytes; cytokeratin 1, u-PAR,and gC1qR were found to be high molecular weight kininogen binding proteins present at the surface of these cells PMID: 14597972
    47. The rate of kallikrein formation by the assembly of factor XII, high molecular weight KNG and PK were similar in both heparinase-treated and non-treated HUVEC, indicating that heparan sulfate does not contribute significantly to HK binding to HUVEC. PMID: 14629481
    48. physically disrupts the formation of a signaling complex containing uPAR, integrin alpha(v)beta3 or alpha5beta1, caveolin, and Src kinase Yes in endothelial cells in a manner that parallels its apoptotic effect PMID: 15044324
    49. ACE-I-induced renoprotection, in the context of macrophage-stimulated mesangial cell scarring, is mediated, at least in part, via the actions of bradykinin. PMID: 15086463
    50. high molecular weight kininogen has an antibacterial domain, called domain 5 PMID: 16091369

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  • 相關(guān)疾病:
    High molecular weight kininogen deficiency (HMWK deficiency)
  • 亞細(xì)胞定位:
    Secreted, extracellular space.
  • 組織特異性:
    Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • 數(shù)據(jù)庫鏈接:

    HGNC: 6383

    OMIM: 228960

    KEGG: hsa:3827

    STRING: 9606.ENSP00000265023

    UniGene: Hs.77741