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  4. Human Kynurenine (KYN)ELISA Kit

Human Kynurenine (KYN)ELISA Kit

  • 中文名稱：
    人犬尿氨酸（KYN)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13659h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人犬尿氨酸（KYN)酶聯(lián)免疫試劑盒（CSB-E13659h）為競爭法ELISA試劑盒，定量檢測血清、尿液、組織勻漿樣本中的KYN含量。試劑盒檢測范圍為7.8 pmol/ml-500 pmol/ml，產(chǎn)品適用于科研場景中KYN代謝機(jī)制研究、疾病模型評估及藥物開發(fā)過程中的生物標(biāo)志物檢測，例如探索神經(jīng)系統(tǒng)疾病動物模型的代謝變化或免疫調(diào)節(jié)藥物對色氨酸代謝通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    KYN
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine, tissue homogenates
  • 檢測范圍：
    7.8 pmol/ml-500 pmol/ml.
  • 靈敏度：
    3.9 pmol/ml.
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human KYN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    95

    Range %

    90-99

    1:2

    Average %

    87

    Range %

    84-90

    1:4

    Average %

    92

    Range %

    88-96

    1:8

    Average %

    97

    Range %

    95-99

  • 回收率：

    The recovery of human KYN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    90-98

    EDTA plasma (n=4)

    92

    87-96

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pmol/ml

    OD1

    OD2

    Average

    500

    0.200

    0.202

    0.201

    250

    0.318

    0.338

    0.328

    125

    0.498

    0.470

    0.484

    62.5

    0.685

    0.664

    0.675

    31.2

    1.036

    1.016

    1.026

    15.6

    1.468

    1.404

    1.436

    7.8

    1.533

    1.657

    1.595

    0

    1.710

    1.723

    1.717

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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