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  4. Human Layilin(LAYN) ELISA kit

Human Layilin(LAYN) ELISA kit

  • 中文名稱：
    人排卵蛋白(LAYN)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL012772HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人排卵蛋白(LAYN)酶聯(lián)免疫試劑盒（CSB-EL012772HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LAYN含量。人排卵蛋白(LAYN)是一種調(diào)節(jié)T細(xì)胞功能的關(guān)鍵基因，其高表達(dá)與多種癌癥的預(yù)后不良相關(guān)，尤其是與胃癌和結(jié)腸癌的免疫浸潤(rùn)水平密切相關(guān)。LAYN可能通過調(diào)節(jié)腫瘤相關(guān)巨噬細(xì)胞、DC、T細(xì)胞耗竭和Treg等免疫細(xì)胞在腫瘤微環(huán)境中的功能，影響腫瘤的進(jìn)展和預(yù)后。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于體外研究LAYN在生殖生理調(diào)控、排卵障礙模型構(gòu)建、卵巢功能評(píng)估以及免疫細(xì)胞相互作用等領(lǐng)域的表達(dá)特征，也可用于篩選調(diào)控LAYN表達(dá)的生物活性物質(zhì)或藥物分子，為生殖醫(yī)學(xué)和免疫學(xué)基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Layilin ELISA Kit; LAYN ELISA Kit; LAYN_HUMAN ELISA Kit; UNQ208/PRO234 ELISA Kit
  • 縮寫：
    LAYN
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human LAYN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    83

    Range %

    80-86

    1:2

    Average %

    92

    Range %

    89-96

    1:4

    Average %

    104

    Range %

    101-107

    1:8

    Average %

    89

    Range %

    85-93

  • 回收率：

    The recovery of human LAYN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    102

    99-106

    EDTA plasma (n=4)

    101

    96-106

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.321

    2.341

    2.331

    2.159

    500

    1.929

    1.902

    1.916

    1.744

    250

    1.395

    1.376

    1.386

    1.214

    125

    0.875

    0.841

    0.858

    0.686

    62.5

    0.574

    0.592

    0.583

    0.411

    31.25

    0.396

    0.387

    0.392

    0.220

    15.6

    0.287

    0.291

    0.289

    0.117

    0

    0.171

    0.173

    0.172

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Receptor for hyaluronate.
  • 基因功能參考文獻(xiàn)：
    1. Renal biopsy samples from patients with glomerulonephritis showed high expression of LAYN in tubular epithelial cells. PMID: 26410531
    2. results indicate that LAYN would be involved in the enhancement of inflammation and degradation of cartilage in joint diseases such as RA and OA PMID: 25150153
    3. Hyaluronan and layilin mediate loss of airway epithelial barrier function induced by cigarette smoke by decreasing E-cadherin. PMID: 23048036
  • 亞細(xì)胞定位：
    Membrane; Single-pass type I membrane protein.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 29471

    KEGG: hsa:143903

    STRING: 9606.ENSP00000364764

    UniGene: Hs.503831