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  4. Human Legumain(LGMN) ELISA kit

Human Legumain(LGMN) ELISA kit

  • 中文名稱：
    人豆莢蛋白(LGMN)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL012903HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人豆莢蛋白(LGMN)酶聯(lián)免疫試劑盒（CSB-EL012903HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LGMN含量。LGMN是一種靶點(diǎn)。它在人體生理中可能發(fā)揮特定作用。研究上，主要聚焦其在相關(guān)疾病發(fā)生發(fā)展機(jī)制中的影響，探尋其與疾病信號(hào)通路、細(xì)胞功能等方面的關(guān)聯(lián)，以此為基礎(chǔ)有望開發(fā)出針對(duì)LGMN的治療策略和藥物。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，靈敏度為15.6 pg/mL。本試劑盒適用于基礎(chǔ)醫(yī)學(xué)研究、藥物開發(fā)篩選及疾病分子機(jī)制探索等場(chǎng)景，為研究LGMN在炎癥反應(yīng)、癌癥微環(huán)境調(diào)控或神經(jīng)病理學(xué)中的作用提供可靠工具，特別適用于需要多類型樣本聯(lián)合分析的實(shí)驗(yàn)設(shè)計(jì)，其寬泛的檢測(cè)范圍可覆蓋生理及病理狀態(tài)下的蛋白表達(dá)水平變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    AEP ELISA Kit; Asparaginyl endopeptidase ELISA Kit; cysteine 1 ELISA Kit; Cysteine protease 1 ELISA Kit; EC 3.4.22.34 ELISA Kit; Legumain ELISA Kit; LGMN ELISA Kit; LGMN_HUMAN ELISA Kit; LGMN1 ELISA Kit; Protease ELISA Kit; Protease cysteine 1 (legumain) ELISA Kit; Protease cysteine 1 ELISA Kit; PRSC1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    517.073

    505.781

    486.170

    497.353

    507.189

    517.073

    SD

    0.030

    0.032

    0.026

    0.038

    0.035

    0.051

    CV(%)

    5.217

    5.644

    4.702

    6.774

    6.162

    8.870

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human LGMN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:2

    Average %

    93

    Range %

    85-102

    1:4

    Average %

    90

    Range %

    84-100

    1:8

    Average %

    95

    Range %

    85-102

    1:16

    Average %

    95

    Range %

    89-102

  • 回收率：

    The recovery of human LGMN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    96

    84-105

    EDTA plasma (n=4)

    97

    81-105

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    4000

    2.300

    2.312

    2.306

    2.155

    2000

    1.395

    1.395

    1.395

    1.244

    1000

    0.886

    0.855

    0.870

    0.719

    500

    0.581

    0.593

    0.587

    0.436

    250

    0.369

    0.351

    0.360

    0.209

    125

    0.260

    0.262

    0.261

    0.110

    62.5

    0.205

    0.216

    0.211

    0.060

    0

    0.154

    0.148

    0.151

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
  • 基因功能參考文獻(xiàn)：
    1. Studies identified Legumain (LGMN) as a new target which is highly expressed in the tumor microenvironment and in tumor cells. [review] PMID: 27993111
    2. our results suggest that M2 tumour-associated macrophages affect degradation of the extracellular matrix and angiogenesis via overexpression of legumain, and therefore play an active role in the progression of DLBCL. PMID: 27464733
    3. Legumain regulates oxLDL-induced macrophage apoptosis by enhancing the autophagy pathway. PMID: 29414692
    4. MiRNA-3978 regulates peritoneal gastric cancer metastasis by targeting legumain expression, promoting cell migration/neoplasm invasiveness. PMID: 27793040
    5. These results showed that Asparaginyl endopeptidase could promote invasion and metastasis by modulating epithelial-to-mesenchymal transition. PMID: 27102302
    6. Our data suggest that altered proteolytic activity of legumain in the bone microenvironment contributes to decreased bone mass in postmenopausal osteoporosis. PMID: 28162997
    7. Legumain is increased in both plasma and plaques of patients with carotid stenosis and might be a new and early biomarker of atherosclerosis. PMID: 27940038
    8. Data suggest that melanoma cells internalize/absorb cystatin C from culture media, leading to increased intracellular cystatin C levels; cystatin E/M is internalized as well but at modest rate due to down-regulation of cell migration; however, the effect of intracellular cystatin E/M on down-regulation of legumain activity is pronounced. PMID: 28630039
    9. AEP is activated and cleaves human alpha-synuclein at N103 in an age-dependent manner. PMID: 28671665
    10. AEP promotes activation of the PI3K-AKT signaling pathway in prostate cancer cells. PMID: 27590439
    11. upregulation of legumain is associated with malignant behavior of uveal melanoma. PMID: 26846877
    12. Studies indicate that legumain, usually in lysosomes, is also found extracellularly and even translocates to the cytosol and the nucleus. PMID: 26403494
    13. legumain might play an important role in cervical cancer cell migration and invasion. PMID: 26802645
    14. Legumain appears to be involved in tumor development and deterioration. PMID: 26607955
    15. AEP acts as a delta-secretase, cleaving APP at N373 and N585 residues, selectively influencing the amyloidogenic fragmentation of APP. AEP contributes to the age-dependent pathogenic mechanisms in Alzheimer disease. PMID: 26549211
    16. High legumain expression is associated with breast cancer. PMID: 25605174
    17. This unique feature was confirmed by the crystal structure of AEPpH4.5 (AEP was matured at pH 4.5 and crystallized at pH 8.5), in which the broken peptide bonds were religated and the structure was transformed back to its proenzyme form. PMID: 24407422
    18. High legumain activity is associated with breast cancer. PMID: 24742492
    19. AEP acts as a crucial mediator of tau-related clinical and neuropathological changes. PMID: 25326800
    20. Identified an alternative oncogenic pathway for TRAF6 that uses AEP as its substrate. AEP and TRAF6 protein levels may have prognostic implications in breast cancer patients. Thus, AEP may serve as a biomarker as well as new therapeutic target PMID: 24610907
    21. the relationship among Legumain expression, clinicopathologic, biological variables and patient prognosis in gastric carcinoma PMID: 24023813
    22. HCT116 cells were transfected with p53 siRNA and the effect of knockdown of p53 expression on legumain expression was examined. The results showed that expression levels of both legumain mRNA and protein were decreased in the siRNA-treated cells. PMID: 23942113
    23. Prostate cancer with a vesicular staining pattern of legumain had the potential of being highly invasive and aggressive in patients treated with radical prostatectomy. This suggests that legumain might contribute to the invasiveness and aggressiveness. PMID: 23124822
    24. The multibranched and context-dependent activation process of legumain illustrates how proteases can act not only as signal transducers but as decision makers. PMID: 23776206
    25. Involvement of brain acidosis in the etiopathogenesis of Alzheimer disease, and the asparaginyl endopeptidase-I2(PP2A)-protein phosphatase 2A-Tau hyperphosphorylation pathway. PMID: 23640887
    26. glycosaminoglycans accelerated the autocatalytic activation of prolegumain PMID: 23160071
    27. In the HCT116 and SW620 cell lines nuclear Data indicate that legumain was found to make up approximately 13% and 17% of the total legumain, respectively. PMID: 23326369
    28. Data show a regulatory role of cystatin E/M in controlling both intra- and extracellular legumain activity. PMID: 22902879
    29. TDP-43 is cleaved by AEP in brain. PMID: 22718532
    30. Increased legumain expression was validated by real-time PCR and Western blots, correlated positively with an increased malignancy of ovarian tumors. PMID: 22441772
    31. Accepting asparagines and, to lesser extent, aspartic acid in P1, super-activated legumain exhibits a marked pH dependence that is governed by the P1 residue of its substrate and conformationally stabilizing factors such as temperature or ligands PMID: 22232165
    32. GARP is a key receptor controlling FOXP3 in T(reg) cells following T-cell activation in a positive feedback loop assisted by LGALS3 and LGMN PMID: 19453521
    33. The Lgmn can serve as novel target in diabetes mellitus genetic therapy. PMID: 20536387
    34. the level of cystatin E/M regulates legumain activity and hence the invasive potential of human melanoma cells PMID: 20074384
    35. Legumain is expressed in both murine and human atherosclerotic lesions, may play a functional role in atherogenesis PMID: 18377911
    36. human monocyte-derived dendritic cells harbor inactive proforms of AEP (legumain) that become activated upon maturation of dendritic cells with lipopolysaccharide PMID: 12860980
    37. Freshly isolated human B lymphocytes lack significant asparagine-specific endoprotease (AEP/legumain) activity; cleavage by AEP is dispensable for proteolytic processing of myelin basic protein in this type of cell. PMID: 15100291
    38. Increased legumain expression is associated with primary colorectal cancer PMID: 15788679
    39. AEP may be a proteinases activated by acidosis triggering neuronal injury during neuroexcitotoxicity or ischemia. PMID: 18374643

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  • 亞細(xì)胞定位：
    Lysosome.
  • 蛋白家族：
    Peptidase C13 family
  • 組織特異性：
    Ubiquitous. Particularly abundant in kidney, heart and placenta.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9472

    OMIM: 602620

    KEGG: hsa:5641

    STRING: 9606.ENSP00000334052

    UniGene: Hs.18069