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  4. Human Leukocyte elastase inhibitor(SERPINB1) ELISA kit

Human Leukocyte elastase inhibitor(SERPINB1) ELISA kit

  • 中文名稱：
    人白細(xì)胞彈性蛋白酶抑制劑(SERPINB1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL021065HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人白細(xì)胞彈性蛋白酶抑制劑(SERPINB1)酶聯(lián)免疫試劑盒（CSB-EL021065HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SERPINB1含量。SERPINB1是一個(gè)重要靶點(diǎn)。它屬于絲氨酸蛋白酶抑制劑家族，在細(xì)胞內(nèi)發(fā)揮調(diào)節(jié)蛋白酶活性的作用。研究發(fā)現(xiàn)其與免疫調(diào)節(jié)、炎癥反應(yīng)等機(jī)制相關(guān)，對維持細(xì)胞內(nèi)環(huán)境穩(wěn)定有重要意義，有可能成為治療炎癥、腫瘤等疾病的新方向。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于體外研究炎癥相關(guān)疾病模型、免疫調(diào)控機(jī)制探索及細(xì)胞功能實(shí)驗(yàn)中目標(biāo)蛋白的動(dòng)態(tài)分析；支持科研人員開展基礎(chǔ)研究中的蛋白表達(dá)量檢測、藥物干預(yù)效果評估或病理生理機(jī)制解析；為探索SERPINB1在先天免疫、組織修復(fù)等領(lǐng)域的生物學(xué)功能提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Anti elastase ELISA Kit; EI ELISA Kit; ELANH2 ELISA Kit; Epididymis luminal protein 57 ELISA Kit; HEL57 ELISA Kit; ILEU_HUMAN ELISA Kit; LEI ELISA Kit; Leukocyte elastase inhibitor ELISA Kit; M/NEI ELISA Kit; MNEI ELISA Kit; Monocyte/neutrophil elastase inhibitor ELISA Kit; Peptidase inhibitor 2 ELISA Kit; Pi-2 ELISA Kit; PI2 ELISA Kit; Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 1 ELISA Kit; Serpin B1 ELISA Kit; Serpin peptidase inhibitor clade B (ovalbumin) member 1 ELISA Kit; serpinb1 ELISA Kit
  • 縮寫：
    SERPINB1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %88-101
    1:2Average %93
    Range %84-97
    1:4Average %91
    Range %85-95
    1:8Average %98
    Range %90-101
  • 回收率：
    The recovery of human SERPINB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10294-107
    EDTA plasma (n=4)9890-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    401.768 1.700 1.734 1.606
    201.409 1.434 1.422 1.294
    100.886 0.944 0.915 0.787
    50.565 0.551 0.558 0.430
    2.50.379 0.363 0.371 0.243
    1.250.245 0.252 0.249 0.121
    0.6250.201 0.197 0.199 0.071
    00.130 0.126 0.128
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity. During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, promotes the proliferation of beta-cells via its protease inhibitory function.
  • 基因功能參考文獻(xiàn)：
    1. The expression of SERPINB1 was approximately 2-fold higher in apical periodontitis. SERPINB1 expression was noted in neutrophils and epithelial cells. PMID: 28673495
    2. SERPINB1 decreased inflammation, ameliorated oxidative stress in the lung, and attenuated acute lung injury in rats with orthotopic autologous liver transplantation by activating HO-1 and it does so through STAT3 PMID: 28427999
    3. a relatively low Serpinb1a protein threshold in neutrophils that is required for sustained survival PMID: 27107834
    4. SERPINB1 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    5. Data show that high serpin B1 protein (SERPINB1) gene expression was associated with favorable tumor response and prolonged survival under cisplatin-based chemotherapy. PMID: 26799424
    6. Data show that oropharyngeal squamous cell carcinomas (OPSCCs) express granzyme inhibitors SERPINB1, SERPINB4 and SERPINB9 for cytotoxicity and the expression was not different between human papillomavirus (HPV)-positive and HPV-negative tumors. PMID: 26993499
    7. Pediatric CNS-PNETs evade immune recognition by downregulating cell surface MHC-I and CD1d expression. Intriguingly, expression of SERPINB9, SERPINB1, and SERPINB4 is acquired during tumorigenesis in 29%, 29%, and 57% of the tumors PMID: 26963506
    8. is not expressed in neutrophils of both sulfur mustard-exposed and chronic obstructive pulmonary disease patients PMID: 24852194
    9. Data suggest that serine protease inhibitor (SERPIN) B1 negatively regulates glioma cell migration and invasion probably by abrogating the expression of matrix metalloproteinase-2 and the activation of focal adhesion kinase. PMID: 24968089
    10. Decreased expression of SERPINB1 correlates with tumor invasion and poor prognosis in hepatocellular carcinoma. PMID: 24105272
    11. Apoptosis-inducing factor and leukocyte elastase inhibitor derived DNase II interact and can cooperate to induce cell death. PMID: 23673989
    12. Upon reactive center loop cleavage at Phe-343,SERPINB1 covalently complexes with GzmH. SERPINB1 overexpression suppresses GzmH- or LAK cell-mediated cytotoxicity. Crystal structures show possible conformational changes in GzmH for the suicide inhibition. PMID: 23269243
    13. In the resting state during human neutrophil extracellar trap generation, SerpinB1 is exclusively in the cytoplasm, consistent with the current understanding of clade B serpins, and it may migrate and regulate events in the cell nucleus. PMID: 23002442
    14. These results suggest that serpin B1 may be a novel marker of active ulcerative colitis and may play an important role in the pathogenesis of inflammatory bowel disease. PMID: 22421620
    15. serpinB1 sustains a healthy neutrophil reserve that is required in acute immune responses. PMID: 21248149
    16. The findings define an innate role for SerpinB1 in cystic fibrosis airways. PMID: 20817705
    17. M/NEI is a dual specificity inhibitor with two adjacent reactive sites that support rapid efficient inhibitory reactions with cellular proteases, including the three neutrophil granule proteases. PMID: 11747453

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  • 亞細(xì)胞定位：
    Secreted. Cytoplasm. Cytolytic granule. Early endosome.
  • 蛋白家族：
    Serpin family, Ov-serpin subfamily
  • 組織特異性：
    In human bone marrow, present in all CD45+ populations. Expression levels are highest in the neutrophil lineage, intermediate in monocytic, and lowest in lymphocytic lineage. Within the neutrophil lineage, expression is highest in promyelocytes.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3311

    OMIM: 130135

    KEGG: hsa:1992

    STRING: 9606.ENSP00000370115

    UniGene: Hs.381167