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Human Lipocalin-15(LCN15) ELISA kit

  • 中文名稱:
    人脂質(zhì)運(yùn)載蛋白-15(LCN15)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL012813HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人脂質(zhì)運(yùn)載蛋白-15(LCN15)酶聯(lián)免疫試劑盒(CSB-EL012813HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的LCN15含量。LCN15(脂質(zhì)運(yùn)載蛋白15)位于人類第9號(hào)染色體,主要在十二指腸、結(jié)腸和其他組織中表達(dá)。其功能與轉(zhuǎn)運(yùn)活性和小分子結(jié)合相關(guān),涉及維生素、核苷和相關(guān)分子的運(yùn)輸以及葡萄糖和其他糖、膽汁鹽和有機(jī)酸、金屬離子和胺化合物的運(yùn)輸。LCN15與某些疾病相關(guān),如逆行性遺忘癥。研究LCN15的機(jī)制有助于理解其在疾病發(fā)生和發(fā)展中的作用。試劑盒檢測(cè)范圍為18.75 pg/mL-1200 pg/mL,適用于基礎(chǔ)科研中探索LCN15在脂質(zhì)轉(zhuǎn)運(yùn)機(jī)制、代謝調(diào)控網(wǎng)絡(luò)或相關(guān)疾病模型中的功能研究;科研人員可利用定量分析不同樣本來源的LCN15含量,為代謝性疾病、肥胖相關(guān)研究或炎癥機(jī)制探索提供可靠的數(shù)據(jù)支持,滿足體外實(shí)驗(yàn)?zāi)P?、?dòng)物研究及生物標(biāo)志物篩選等科研需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    LCN15 ELISA Kit; UNQ2541/PRO6093 ELISA Kit; Lipocalin-15 ELISA Kit
  • 縮寫:
    LCN15
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    18.75 pg/mL-1200 pg/mL
  • 靈敏度:
    4.68 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human LCN15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-96
    1:2 Average % 90
    Range % 86-97
    1:4 Average % 96
    Range % 90-102
    1:8 Average % 94
    Range % 91-98
  • 回收率:
    The recovery of human LCN15 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-99
    EDTA plasma (n=4) 87 85-96
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1200 2.083 2.252 2.168 2.038
    600 1.648 1.699 1.674 1.544
    300 1.144 1.165 1.155 1.025
    150 0.692 0.721 0.707 0.577
    75 0.446 0.457 0.452 0.322
    37.5 0.308 0.326 0.317 0.187
    18.75 0.215 0.225 0.220 0.090
    0 0.129 0.131 0.130  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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