Human Macroprolactin ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人巨泌乳素酶聯(lián)免疫試劑盒（CSB-E16222h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MPRL含量。人巨泌乳素是一種由垂體前葉分泌的激素，主要功能是刺激乳汁生成和分泌。其靶點(diǎn)背景涉及多種生物過程，包括生殖和母乳喂養(yǎng)。研究機(jī)制顯示，人巨泌乳素通過其受體PRLR發(fā)揮作用，影響細(xì)胞增殖、分化、分泌和生存，并在多種生理和病理生理過程中扮演重要角色。試劑盒檢測范圍為0.4 μIU/mL-20 μIU/mL，可廣泛用于科研領(lǐng)域中對巨泌乳素表達(dá)水平的動態(tài)監(jiān)測，例如探究其在細(xì)胞分泌調(diào)控、組織生理病理狀態(tài)中的生物學(xué)功能，或用于體外實(shí)驗(yàn)?zāi)Ｐ椭兴幬锔深A(yù)、基因編輯等因素對巨泌乳素合成與釋放的影響機(jī)制研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

MPRL
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.4 μIU/mL-20 μIU/mL
靈敏度：

0.5 μIU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human macroprolactin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-98
1:2	Average %	89
	Range %	84-99
1:4	Average %	95
	Range %	91-102
1:8	Average %	95
	Range %	90-103

回收率：

The recovery of human macroprolactin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-102
EDTA plasma (n=4)	97	87-108

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.