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  4. Human Mast Cell Carboxypeptidase ELISA Kit

Human Mast Cell Carboxypeptidase ELISA Kit

  • 中文名稱：
    人肥大細(xì)胞類羧肽酶酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13756h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人肥大細(xì)胞類羧肽酶酶聯(lián)免疫試劑盒（CSB-E13756h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CPA3含量。CPA3是一個(gè)值得關(guān)注的靶點(diǎn)。它在人體生理與病理過程中發(fā)揮作用。目前研究聚焦于其參與的免疫反應(yīng)、炎癥調(diào)節(jié)等機(jī)制。了解其作用機(jī)制有助于深入認(rèn)識(shí)相關(guān)疾病發(fā)病原理，為開發(fā)針對(duì)性治療手段如藥物研發(fā)等提供重要方向。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于科研領(lǐng)域中對(duì)肥大細(xì)胞相關(guān)疾病機(jī)制的研究，如過敏性疾病模型構(gòu)建、炎癥標(biāo)志物分析以及藥物干預(yù)下CPA3表達(dá)水平變化的評(píng)估，為探索肥大細(xì)胞功能調(diào)控提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    carboxypeptidase A ELISA Kit; carboxypeptidase A3 (mast cell) ELISA Kit; Carboxypeptidase A3 ELISA Kit; carboxypeptidase A3 mast cell ELISA Kit; CBPA3_HUMAN ELISA Kit; Cpa3 ELISA Kit; Mast cell carboxypeptidase A ELISA Kit; mast cell carboxypeptidase A3 ELISA Kit; MC CPA ELISA Kit; MC-CPA ELISA Kit; tissue carboxypeptidase A ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MC-CPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %100
    Range %94-103
    1:4Average %97
    Range %94-100
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of human MC-CPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9694-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.477 2.453 2.465 2.366
    101.993 1.924 1.959 1.860
    51.280 1.271 1.276 1.177
    2.50.685 0.678 0.682 0.583
    1.250.433 0.424 0.429 0.330
    0.6250.286 0.269 0.278 0.179
    0.3120.190 0.179 0.185 0.086
    00.101 0.097 0.099 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. Carboxypeptidase A3 was differentially expressed between Fibromyalgia patients and healthy controls. PMID: 27157394
    2. Urine CPA3 was increased in patients with chronic prostatitis/chronic pelvic pain syndrome compared to controls. PMID: 24726923
    3. The cDNA encoding human colon mast cell carboxypeptidase can be successfully cloned and expressed in E. coli and P. pastoris. PMID: 14760754
    4. Role in regulating innate immunity responses and regulating secretory granule homeostasis PMID: 19643669
  • 亞細(xì)胞定位：
    Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules.
  • 蛋白家族：
    Peptidase M14 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2298

    OMIM: 114851

    KEGG: hsa:1359

    STRING: 9606.ENSP00000296046

    UniGene: Hs.646