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  4. Human Maternal embryonic leucine zipper kinase(MELK) ELISA kit

Human Maternal embryonic leucine zipper kinase(MELK) ELISA kit

  • 中文名稱：
    人母體胚胎亮氨酸拉鏈激酶(MELK)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL013692HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人母體胚胎亮氨酸拉鏈激酶(MELK)酶聯(lián)免疫試劑盒（CSB-EL013692HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MELK含量。MELK（母體胚胎亮氨酸拉鏈激酶）是一種在多種惡性腫瘤中高表達(dá)的蛋白，作為新型分子靶向治療靶點(diǎn)，其通過與多種蛋白相互作用調(diào)節(jié)靶蛋白，在腫瘤增殖、遷移和干細(xì)胞特性維持中起關(guān)鍵作用。研究顯示，MELK在神經(jīng)干細(xì)胞和腦腫瘤干細(xì)胞自我更新中不可或缺，其功能失調(diào)與癌細(xì)胞逃避免疫監(jiān)視有關(guān)。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域，如腫瘤生物學(xué)機(jī)制研究、干細(xì)胞功能探索、抗癌藥物開發(fā)中的靶點(diǎn)活性評估等場景，為體外實(shí)驗(yàn)提供可靠的定量檢測工具。實(shí)驗(yàn)操作流程標(biāo)準(zhǔn)化，兼容常規(guī)酶標(biāo)儀檢測，滿足實(shí)驗(yàn)室對多種生物樣本的分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    AI327312 ELISA Kit; hMELK ELISA Kit; HPK 38 ELISA Kit; hPK38 ELISA Kit; KIAA0175 ELISA Kit; Likely ortholog of maternal embryonic leucine zipper kinase ELISA Kit; Maternal embryonic leucine zipper kinase ELISA Kit; MELK ELISA Kit; MELK_HUMAN ELISA Kit; mKIAA0175 ELISA Kit; MPK38 ELISA Kit; OTTHUMP00000021377 ELISA Kit; OTTHUMP00000046113 ELISA Kit; pEg3 kinase ELISA Kit; Protein kinase Eg3 ELISA Kit; Protein kinase PK38 ELISA Kit; RP23 382O11.1 ELISA Kit; Tyrosine protein kinase MELK ELISA Kit
  • 縮寫：
    MELK
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MELK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %86
    Range %80-93
    1:4Average %96
    Range %90-101
    1:8Average %100
    Range %96-105
  • 回收率：
    The recovery of human MELK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9488-99
    EDTA plasma (n=4)9589-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.776 2.863 2.820 2.671
    52.121 1.995 2.058 1.909
    2.51.142 1.241 1.192 1.043
    1.250.705 0.733 0.719 0.570
    0.6250.393 0.387 0.390 0.241
    0.3120.296 0.284 0.290 0.141
    0.1560.238 0.245 0.242 0.093
    00.150 0.148 0.149 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
  • 基因功能參考文獻(xiàn)：
    1. Study demonstrates that the interaction occurring between MELK and EZH2 promotes self-proliferation and stemness. PMID: 28536141
    2. In common culture conditions, the authors found that small molecule inhibition, genetic deletion, or acute depletion of MELK did not significantly affect cellular growth. PMID: 28926338
    3. Synthesis of MCL1, an antiapoptotic protein known to play a role in cancer cell survival during cell division, depends on the function of MELK-elF4B signaling. PMID: 27528663
    4. Inhibition of MELK (genetically and pharmacologically) induces radiation sensitivity. PMID: 27225691
    5. MELK is a host factor required for optimal uncoating of the HIV-1 core to promote viral cDNA synthesis. PMID: 28683086
    6. Here, the authors report that mutagenizing MELK with CRISPR/Cas9 has no effect on the fitness of basal breast cancer cell lines or cell lines from six other cancer types. Cells that harbor null mutations in MELK exhibit wild-type doubling times, cytokinesis, and anchorage-independent growth. PMID: 28337968
    7. MELK-inhibitor has a role in triple-negative breast cancer cells demonstrating context-dependent response with p53 as a key determinant PMID: 28235006
    8. MELK is an oncogenic kinase involved in the pathogenesis and recurrence of hepatocellular carcinoma. PMID: 27693640
    9. Inhibition or depletion of MELK reduced cell proliferation and anchorage-dependent and -independent growth in various ovarian cancer cell lines through a G2/M cell cycle arrest, eventually resulting in apoptosis. PMID: 28214016
    10. Report IL11RA and MELK amplification in gastric cancer cell lines and primary gastric adenocarcinomas. PMID: 27920471
    11. MELK expression in hepatocellular carcinoma is extremely intense compared to its expression reported in other types of cancer and could be a promising effective tumor marker of HCC. PMID: 27798878
    12. targeting MELK by the inhibition of both its catalytic activity and its protein stability might sensitize tumours to DNA-damaging agents or radiation therapy by lowering the DNA-damage threshold PMID: 26431963
    13. Together, these data indicate that MELK is a normally non-essential kinase, but is critical for basal-like breast cancer. PMID: 24844244
    14. EZH2 protects glioma stem cells from radiation-induced cell death in a MELK/FOXM1-dependent manner PMID: 25601206
    15. we report characterization of possible roles of MELK in acute myeloid leukemia PMID: 25365263
    16. insight has been brought by the discovery of a protein complex of FOXM1 with the mitotic kinase MELK in cancer stem cells in brain cancers, as this protein complex appears to be cancer-specific PMID: 25017123
    17. advanced cancers with OTSSP167 started in 2013, as the first-in-class MELK inhibitor. This review summarizes the current molecular understanding of MELK and the recent preclinical studies about MELK as a cancer therapeutic target. PMID: 24795222
    18. MELK promotes cell migration and invasion via the FAK/Paxillin pathway, and plays an important role in the occurrence and development of gastric cancer. PMID: 24885567
    19. our current knowledge of MELK function and recent discoveries in MELK signaling pathway were discussed. PMID: 24185907
    20. The structural and biochemical analyses unravel the molecular mechanisms for the autophosphorylation/activation of MELK and the dependence of its catalytic activity on reducing agents. PMID: 23922895
    21. Report MELK inhibitor that suppresses the growth of a wide range of human tumor cell lines. PMID: 23283305
    22. Data indicate that expression-based risk indices of three genes UBE2C, TPX2, and MELK were more strongly associated with poor 5-year survival in adenocarcinoma patients. PMID: 23357462
    23. MELK is upregulated in high-grade prostate cancer PMID: 22945237
    24. MELK could be associated with increased resistance of colorectal cancer cells against radiation and 5-FU. PMID: 21806965
    25. High MELK is associated with brain tumor. PMID: 21558073
    26. MELK expression is increased in breast cancer tissue and this is associated with poor survival. The most important factors controlling Bcl-G activity are post-translational modification by Fau & MELK. PMID: 19671159
    27. pEg3 is a potential regulator of the G2/M progression and may act antagonistically to the CDC25B phosphatase,pEg3 kinase is able to specifically phosphorylate CDC25B in vitro. One phosphorylation site was identified and corresponded to serine 323 PMID: 12400006
    28. analysis of MELK substrate specificity and activity regulation PMID: 16216881
    29. the kinase activity of MELK is likely to affect mammary carcinogenesis through inhibition of the pro-apoptotic function of Bcl-GL PMID: 17280616
    30. a critical role for MELK in the proliferation of brain tumors, including their stem cells, and suggest that MELK may be a compelling molecular target for treatment of high-grade brain tumors. PMID: 17722061
    31. Maternal embryonic leucine zipper kinase transcript abundance correlates with malignancy grade in astrocytomas PMID: 17960622

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  • 相關(guān)疾?。?/div>
    Defects in MELK are associated with some cancers, such as brain or breast cancers. Expression is dramatically increased in aggressive undifferentiated tumors, correlating with poor patient outcome in breast and brain cancers, suggesting a role in tumor-initiating cells and proliferation via its function in cell proliferation regulation.
  • 亞細(xì)胞定位：
    Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
  • 組織特異性：
    Expressed in placenta, kidney, thymus, testis, ovary and intestine.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 16870

    OMIM: 607025

    KEGG: hsa:9833

    STRING: 9606.ENSP00000298048

    UniGene: Hs.184339