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  4. Human Matrix Gla Protein,MGP ELISA Kit

Human Matrix Gla Protein,MGP ELISA Kit

  • 中文名稱：
    人基質(zhì)γ羧基谷氨酸蛋白(MGP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09714h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人基質(zhì)γ羧基谷氨酸蛋白(MGP)酶聯(lián)免疫試劑盒（CSB-E09714h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MGP含量。MGP（羧基谷氨酸蛋白）是一種在骨骼中發(fā)現(xiàn)的內(nèi)源性小蛋白，具有抑制血管和軟骨鈣化的功能。其在卵巢癌中的表達與腫瘤免疫浸潤和患者預(yù)后密切相關(guān)，研究顯示MGP高表達與患者預(yù)后差密切相關(guān)，并可能通過調(diào)節(jié)免疫相關(guān)信號通路影響腫瘤的發(fā)生發(fā)展。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于體外培養(yǎng)細胞模型中鈣化機制研究、動物實驗組織樣本的病理學評估，以及臨床前研究中生物標志物表達水平的檢測，為心血管疾病、骨質(zhì)疏松等領(lǐng)域的科研工作提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cell growth inhibiting gene 36 protein ELISA Kit; Cell growth-inhibiting gene 36 protein ELISA Kit; GAMMA-CARBOXYGLUTAMIC ACID PROTEIN, MATRIX ELISA Kit; GIG36 ELISA Kit; Matrix Gla protein ELISA Kit; MGLAP ELISA Kit; MGP ELISA Kit; MGP_HUMAN ELISA Kit; NTI ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %100
    Range %95-115
    1:200Average %97
    Range %91-111
    1:400Average %97
    Range %92-106
    1:800Average %93
    Range %86-98
  • 回收率：
    The recovery of human MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)10196-110
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.712 2.778 2.745 2.641
    2502.206 2.231 2.219 2.115
    1251.507 1.537 1.522 1.418
    62.50.895 0.904 0.900 0.796
    31.20.457 0.496 0.477 0.373
    15.60.254 0.289 0.272 0.168
    7.80.162 0.169 0.166 0.062
    00.101 0.106 0.104 　
  • 本試劑盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human MGP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled MGP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent(50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

相關(guān)問答

■ 常見問題解答
Q:

我想咨詢一下試用試劑盒和完整試劑盒之間的區(qū)別，以確保我們將得到相同的結(jié)果。尤其是對于Human Matrix Gla Protein, MGP ELISA Kit 和 Human Osteocalcin/Bone gla protein, OT/BGP ELISA kit。

A:
我們很難保證測試結(jié)果完全一致。結(jié)果受許多因素的影響，如不同批次之間的細微差異，誤差因素等。有隨機誤差和疏忽誤差(這樣的因素并不多，但不能排除)，隨機誤差只能盡量最小化，不能完全避免。 許多客戶在試用了我們的24T樣品試劑盒后訂購了96T全套樣品，沒有人抱怨96T全套樣品，這證明我們的ELISA試劑盒使用情況良好。

靶點詳情

  • 功能：
    Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
  • 基因功能參考文獻：
    1. Dp-ucMGP was high preoperatively, and further increased postoperatively in cardiovascular risk patients. PMID: 29303985
    2. Study found an association between diastolic left ventricular function with circulating inactive desphospho-uncarboxylated MGP in two cohorts; undertook histological studies confirming the presence of MGP in the heart and determining its exact localization in healthy and diseased hearts, using conformation-specific MGP antibodies. PMID: 29529056
    3. Studied serum levels of bone morphogenic protein-4 (BMP-4) and matrix Gla protein (MGP) in patients who were admitted to emergency department with the diagnosis of acute coronary syndrome (ACS) and underwent primary percutaneous coronary intervention. MGP and BMP-4 levels were significantly elevated when compared to subjects with normal coronary arteries. PMID: 28605143
    4. genetic association studies in population in Greece: Data suggest that an SNP in promoter region of matrix Gla protein (rs1,800,802; T-138C) is associated with diabetic angiopathy (as indicated by carotid intima media thickness) in patients with type 2 diabetes and diabetic nephropathy. PMID: 28734846
    5. This study concluded that diabetes coexisting with renal disease leads to extreme vascular calcification expressed by elevated MGP levels, resulting in higher frequency of cardiovascular disease in comparison to CKD patients without diabetes. PMID: 28654853
    6. Increased desphospho-uncarboxylated matrix Gla protein, which is a circulating biomarker of vitamin K status and vascular calcification, is independently associated with aortic stiffness, but not with stiffness of distal muscular-type arteries. PMID: 26016598
    7. Our results indicate that the association between the matrix Gla protein variant and increased risk for hand Osteoarthritis is caused by a lower expression of matrix Gla protein, which may increase the burden of hand Osteoarthritis by decreased inhibition of cartilage calcification. PMID: 28855172
    8. Genetic variability in the MGP gene was associated with vascular recurrence in the Spanish population. PMID: 28411264
    9. MGP is significantly repressed by miR-155 in breast cancer MCF-7 cells, and concomitantly there is a stimulation of cell proliferation and cell invasiveness. PMID: 27009385
    10. ectopic expression of Mgp in murine and human osteosarcoma cells led to a marked increase in lung metastasis. Abrogation of Mgp prevented lung metastatic activity, an effect that was rescued by forced expression. Mgp levels dramatically altered endothelial adhesion, trans-endothelial migration in vitro and tumour cell extravasation ability in vivo PMID: 27172275
    11. MGP rs4236 [A/G] gene polymorphism was not to be associated with subgingival dental calculus. Also, that GCF MGP levels were detected higher in patients with subgingival dental calculus than those without subgingival dental calculus independently of polymorphism, may be the effect of adaptive mechanism to inhibit calculus formation. PMID: 27348051
    12. The substitution of threonine by alanine due to MGP exon 4 Thr83Ala polymorphism is related to a decrease in the likelihood of arterial calcification in female persons in the Ukrainian population PMID: 27119839
    13. Correlations of plasma desphosphorylated uncarboxylated matrix Gla protein (dp-ucMGP) with vascular calcification and vascular stiffness in chronic kidney disease. Plasma dp-ucMGP was positively associated with vascular calcification and might be utilized as an early marker for vascular calcification in CKD patients. PMID: 27951533
    14. circulating dp-ucMGP and dp-cMGP may have a role in increasing risk of all-cause and cardiovascular mortality PMID: 24835435
    15. It is assumed that Lp-PLA2 is involved in vascular calcification and that dp-ucMGP is a more appropriate biomarker of residual risk than Lp-PLA2 itself. PMID: 25458708
    16. Data shoed increased GAS6 and decreased MGP levels in hemodialysis patients, as mediators of induction or prevention of vascular calcification. PMID: 25957430
    17. Altering NOTCH1 levels affected MGP mRNA and protein. PMID: 25871831
    18. Uncarboxylated MGP in synovial fluid might serve as a novel biomarker for assessing knee osteoarthritis progression. PMID: 26771974
    19. MGP expression is significantly lower in diseased relative to normal aortic valve interstitial cells. Lack of this important "anti-calcification" protein may contribute to calcification of the aortic valve. PMID: 25990696
    20. Higher plasma dephosphorylated uncarboxylated MGP (reflective of lower vitamin K status) was associated with higher odds of meniscus damage, osteophytes, bone marrow lesions, and subarticular cysts. PMID: 25528106
    21. High levels of desphospho-uncarboxylated MGP are independently and positively associated with arterial stiffness after adjustment for common cardiovascular risk factors, renal function, and age. PMID: 25987667
    22. The association has been found between the ischemic atherothrombotic stroke and polymorphic variants of genes MGP and VKORC1. PMID: 26040031
    23. MGP expression is impaired in patients with ankylosing spondylitis PMID: 25974989
    24. The concentration of dephosphorylated-uncarboxylated Matrix Gla protein was higher in hemodialysis patients treated with vitamin K. PMID: 25190488
    25. the findings of this prospective study among type 2 diabetic patients shows that high circulating dpucMGP levels are associated with increased cardiovascular diseases risk, especially with peripheral arterial disease and heart failure. PMID: 23877986
    26. Matrix Gla protein gene functional polymorphisms is associated with loss of bone mineral density and progression of aortic calcification. PMID: 24281054
    27. Inactive nonphosphorylated and uncarboxylated matrix Gla protein levels were followed in a Flemish population. They correlated causally with non-cancer mortality and coronary events but not total cardiovascular mortality. PMID: 25421980
    28. Both mutations predict complete loss of MGP function. PMID: 24458983
    29. High matrix Gla protein levels are associated with below-knee arterial calcification score in type 2 diabetes mellitus patients. PMID: 24762216
    30. Vitamin K insufficiency, as assessed by high plasma matrix Gla protein concentrations, is associated with increased risk for cardiovascular disease independent of classical risk factors and vitamin D status. PMID: 24210635
    31. Based on the study results, the MGP protein did not play an important role in the development of stenosis of coronary arteries. PMID: 24445527
    32. The MGP-138CC genotype may be associated with slower progression of vascular calcification in maintenance hemodialysis patients PMID: 23504408
    33. High MGP concentrations may be associated with more vascular calcification. PMID: 24029658
    34. Higher circulating MGP levels could help identify minor carotid stenosis with calcification in a relatively homogenous risk population (i.e., postmenopausal women), regardless of underlying cardiovascular risk factors PMID: 22992285
    35. The A/A-variant of MGP gene is associated with an increased risk of ischemic atherothrombotic stroke in the Ukrainian women. PMID: 24228496
    36. rs1800802 (T > C) polymorphism within the MGP promoter is not related to stenosis of the coronary artery. PMID: 23563003
    37. Genetic variants of MGP are associated with calcification on the arterial wall. PMID: 23677904
    38. Circulating dp-ucMGP and t-ucMGP may serve as markers for the extent of coronary artery calcification, but these findings need to be confirmed PMID: 22819559
    39. MGP is a multi-functional inhibitor of normal and abnormal angiogenesis that may function by coordinating with both Notch and BMP signaling pathways PMID: 23110920
    40. MGP carboxylation remained much less in pseudoxanthoma elasticum fibroblasts. PMID: 23223140
    41. Menaquinone supplementation dose-dependently decreases dephospho-uncarboxylated MGP concentrations, but does not affect other MGP species. PMID: 23062766
    42. Increased risk of myocardial infarction associated with MGP ThrAla83 genotype observed elsewhere may be related to faster progression of subclinical coronary atherosclerosis. PMID: 23307874
    43. findings showed that SNPrs4236 of the MGP gene is associated with kidney stones in the Chinese Han population, and influences the genetic susceptibility to kidney stones PMID: 23046575
    44. the A/A-variant of MGP gene promotor is associated with an increased risk of ischemic atherothrombotic stroke in female persons in the Ukrainian population. PMID: 23233942
    45. The change in serum fetuin-A, matrix Gla protein (MGP), and osteopontin (OPN) levels after intracerebral hemorrhage (ICH) indicates that these parameters play a role in the pathophysiological processes leading to an ICH. PMID: 22115341
    46. Angiotensin II exacerbates vascular calcification through activation of transcription factors, and regulation of MGP and inflammatory cytokine expression in vascular smooth muscle cells. PMID: 22796540
    47. There may be an association between hand osteoarthritis and genetic polymorphism at the matrix Gla protein (MGP) locus that is not reflected by total MGP serum concentrations. PMID: 21724703
    48. MGP level correlated negatively with proinflammatory cytokines & acute phase proteins in acute pancreatitis, & positively with lipase, fetuin A, & albumin, indicating a possible role in calcium & phosphate metabolism disturbances in AP. PMID: 22239033
    49. a dysregulated MGP system could be involved in left ventricular dysfunction in patients with chronic heart failure PMID: 21294711
    50. Mgp gene deletion may have a role in arteriovenous malformations PMID: 21765215

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  • 相關(guān)疾?。?/div>
    Keutel syndrome (KTLS)
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Osteocalcin/matrix Gla protein family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 7060

    OMIM: 154870

    KEGG: hsa:4256

    UniGene: Hs.365706