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Human Matrix metalloproteinase 7,MMP-7 ELISA kit

  • 中文名稱:
    人基質(zhì)金屬蛋白酶7(MMP-7)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E04679h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人基質(zhì)金屬蛋白酶7(MMP-7)酶聯(lián)免疫試劑盒(CSB-E04679h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MMP7含量。MMP7 即基質(zhì)金屬蛋白酶 7,在腫瘤發(fā)生、發(fā)展及轉(zhuǎn)移等過程中扮演重要角色。其研究機(jī)制主要聚焦于它對細(xì)胞外基質(zhì)的降解作用,可破壞組織屏障,促進(jìn)腫瘤細(xì)胞的遷移與侵襲,也是潛在的腫瘤診斷和治療靶點(diǎn),相關(guān)研究持續(xù)深入。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,靈敏度為19.5 pg/mL。適用于體外研究場景,如腫瘤微環(huán)境分析、疾病相關(guān)分子機(jī)制探索、藥物干預(yù)效果評估或生物標(biāo)志物篩選等科研領(lǐng)域,為分子生物學(xué)、免疫學(xué)及轉(zhuǎn)化醫(yī)學(xué)研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Matrilysin ELISA Kit; Matrin ELISA Kit; Matrix Metalloproteinase 7 ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP 7 ELISA Kit; MMP-7 ELISA Kit; MMP7 ELISA Kit; MMP7_HUMAN ELISA Kit; MPSL1 ELISA Kit; PUMP 1 ELISA Kit; Pump 1 protease ELISA Kit; Pump-1 protease ELISA Kit; PUMP1 ELISA Kit; Uterine matrilysin ELISA Kit; Uterine metalloproteinase ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 pg/mL-5000 pg/mL
  • 靈敏度:
    19.5 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    626.781

    621.749

    625.103

    627.619

    625.942

    623.426

    SD

    0.041

    0.039

    0.042

    0.054

    0.058

    0.056

    CV(%)

    5.093

    4.881

    5.230

    6.700

    7.214

    6.991

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    104

    Range %

    100-108

    1:2

    Average %

    88

    Range %

    84-92

    1:4

    Average %

    90

    Range %

    85-98

    1:8

    Average %

    92

    Range %

    88-96

  • 回收率:

    The recovery of human MMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    90-101

    Heparin plasma (n=4)

    93

    89-97

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.715

    2.625

    2.670

    2.526

    2500

    2.156

    2.249

    2.203

    2.059

    1250

    1.435

    1.384

    1.410

    1.266

    625

    0.860

    0.799

    0.830

    0.686

    312

    0.498

    0.467

    0.483

    0.339

    156

    0.289

    0.271

    0.280

    0.136

    78

    0.215

    0.211

    0.213

    0.069

    0

    0.145

    0.142

    0.144

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    MMP7,全名為基質(zhì)金屬蛋白酶-7,也稱為哺乳動物型表皮鱗狀細(xì)胞生長因子相關(guān)蛋白(MT-MMP1)。它是一種參與膠原蛋白降解和清除過程的酶類分子,同時也參與組織重塑、血管生成和免疫反應(yīng)等多種生理和病理過程。最近的研究表明,MMP7在多種癌癥的發(fā)生和發(fā)展中發(fā)揮重要作用,成為了一個重要的治療靶點(diǎn)。此外,MMP7還與炎癥性腸病、腎臟病等疾病的發(fā)生和發(fā)展密切相關(guān),成為了重要的生物標(biāo)志物和疾病預(yù)測因子。
  • 功能:
    Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • 基因功能參考文獻(xiàn):
    1. CTHRC1 serves as a pro-metastatic gene that contributes to NSCLC invasion and metastasis, which are mediated by upregulated MMP7 and MMP9 expression. Targeting CTHRC1 may be beneficial for inhibiting NSCLC metastasis. PMID: 29631554
    2. no difference in the expression of MMP-7 and TIMP-1 in the endometrium in relation to hormone levels and menstrual cycle phases were observed PMID: 30187906
    3. MMP-7 A-181G may play an indirect role in determining personal susceptibility to Colorectal Cancer. PMID: 29695403
    4. Results provide evidence that MMP7 expression is regulated by leptin through ERK and JNK pathways to modulate cell invasion of ovarian neoplasm. PMID: 28885729
    5. Absence of MMP-7 Promoter Polymorphisms is associated with Lung Cancer Susceptibility. PMID: 30275186
    6. MMP-7(-181A/G) polymorphisms associate with obesity risk and its severity. PMID: 29317790
    7. Higher serum MMP-7 levels were independently associated with renal fibrosis and poor prognosis in IgA Nephropathy. PMID: 28922659
    8. The results demonstrate that MMP-7 may play an important role in the defensive mechanism against the aggregation of amyloid beta (1-42), which gives rise to the pathology of Alzheimer's disease. PMID: 28871443
    9. among HPV-positive OPSCC patients, high MMP-7 expression is related to worse 5-year DSS and increased rate of distant recurrences PMID: 29721609
    10. MMP7 promoter genotypes only play an indirect role in determining the personal susceptibility to oral cancer in Taiwan. PMID: 29599326
    11. S1P induces advanced tumor phenotypes of hepatocellular carcinoma via establishing an MMP-7/syndecan-1/TGF-beta1 autocrine loop PMID: 27556509
    12. the anti-tumor activity of oleuropein against hepatocellular carcinoma could be attributed to influencing the pro-NGF/NGF balance via affecting MMP-7 activity without affecting the gene expression of NGF PMID: 29476769
    13. These findings suggest the usefulness of combining MMP-7 with CA 125 and HE4 in the diagnosis of epithelial ovarian cancer as a new tumor marker panel. PMID: 28662671
    14. Our findings suggest that the two MMP-7 polymorphisms A-181G and C-153T do not play a major role in determining personal susceptibility to renal cell cancer in Taiwan PMID: 28652430
    15. results suggest that MMP-7 might be involved in the pathogenesis of H. pylori; peptic ulcer was associated with cag pathogenicity island-dependent MMP-7 upregulation PMID: 28699830
    16. Studies have identified an unexpected tumor-suppressive role for host-derived MMP-7 in myeloma bone disease in vivo, and highlight the importance of elucidating the effect of individual MMPs in a disease-specific context. PMID: 28241871
    17. the crosstalk between ARF and MMP7 in nucleus contributes to ECM network in tumor microenvironments in vivo, implicating a novel therapeutic target for advanced PCa treatment. PMID: 27356744
    18. Data suggest that HAI1, a protease on the surface of colon carcinoma cells, is an MMP7 substrate; proteolysis by MMP7 releases extracellular region as soluble HAI1 (sHAI1); sHAI1 induces cancer cell aggregation; cholesterol sulfate is required for MMP-7--catalyzed generation of sHAI1. (HAI1 = hepatocyte growth factor activator inhibitor type 1; MMP7 = matrix metalloproteinase-7) PMID: 29046355
    19. Results indicate that the matrix metallopeptidase 7 (MMP7)A-181G genotype interacts with age and gender and may serve as an early and predictive biomarker for childhood acute lymphoblastic leukemia (ALL). PMID: 29187444
    20. SLC12A5 promoted the migration and invasion of BUC by enhancing MMP-7 expression. PMID: 28333147
    21. Matrix Metalloproteinase-7 Promoter polymorphism is associated with breast Cancer. PMID: 28870920
    22. Data suggest that the cytoplasmic domain of Sdc2 is involved in regulation of expression of MMP7 in colon carcinoma/adenocarcinoma cells; induction of MMP7 involves protein kinase C gamma-mediated FAK/ERK signaling. (Sdc2 = syndecan-2; MMP7 = matrix metalloproteinase-7; FAK = focal adhesion kinase 1) PMID: 28821612
    23. We conclude that in the resected esophageal cancer an increased mRNA expression of MMP-7, MMP-10 and TIMP-1 correlated with clinicopathologic features. We suggest that these genes may play a role during progression of the disease MMP-10, MMP-7, TIMP-1, TIMP-2 were overexpressed in 73%, 85%, 55% and 42% of esophageal cancer samples, respectively. PMID: 28510611
    24. plasma concentrations of MMP-7, MMP-8, -9 and TIMP-1 within 96 h from the onset of acute pancreatitis symptoms are elevated in acute pancreatitis patients compared with healthy controls PMID: 27561093
    25. ZnCo-heterobimetallic analog of cdMMP7 with Co(II) bound in the catalytic site was prepared and characterized. This study describes a well-characterized analog of MMP7 that is available for future inhibitor design efforts. PMID: 27755977
    26. data suggest that syndecan-2 induces extracellular shedding of E-cadherin and supports the acquisition of a fibroblast-like morphology by regulating MMP-7 expression in a colon cancer cell line PMID: 27270030
    27. There were no statistically significant differences in the distribution of MMP7 -181 A/G and MMP12 -82 A/G genotype, allele, or haplotype frequencies between IRSA patients and controls, as well as patients' primary and secondary idiopathic recurrent spontaneous abortion PMID: 27987113
    28. No significant differences were found between MMP-7 A-181G, C-115T, and TIMP-2 G-418C polymorphism and coronary artery disease and myocardial infarction in a Turkish population. PMID: 28137415
    29. MMP-7 is a stable neutral hydrophilic secreted protein, and it may play a vital role in the invasion and metastasis of cancer cells. PMID: 27146730
    30. MMP-7 expression is regulated by SOX18 in prostate cancer. PMID: 27922675
    31. Elevated levels of MMP-7 are Associated with Idiopathic Pulmonary Fibrosis. PMID: 27293304
    32. High MMP2 expression is associated with necrosis in Pancreatic Ductal Adenocarcinoma. PMID: 27429508
    33. Together, these data suggest that IDH2 may be a tumor suppressor in that its loss may promote malignant progression of gastric cancer via NF-kappaB-dependent increases in MMP7 activity. PMID: 26553362
    34. Expression of MMP7 was increased in CRC tissues and cell lines, and inversely correlated with miR-143 expression in CRC tissues. Increased expression of miR-143 repressed MMP7 expression in CRC cells both in mRNA and protein levels. Increased expression of MMP7 reversed the potential influence of miR-143 on CRC cell proliferation and invasion ability. PMID: 27827523
    35. In conclusion, individuals with -181GG genotype and G allele had no impact on susceptibility to the development of HIV-associated neurocognitive disorder and its severity. PMID: 27538541
    36. Together, perlecan fragments in sera and MMP-7 in tissues of Prostate cancer patients are measures of invasive Prostate cancer. PMID: 26862737
    37. The prevalence of matrix metallopeptidase 7 G allele was 40% in studied individuals. PMID: 26950446
    38. Data suggest that, in type 2 diabetes complicated by kidney disease, up-regulation of urinary MMP7 is strongly associated with progression to end-stage renal disease/subsequent mortality; study was conducted in public hospitals in Los Angeles County. PMID: 26412030
    39. Data suggest MMP7 (matrix metalloproteinase 7) in follicular fluid cleaves proNGF (pro-nerve growth factor) in ovarian follicle; both MMP7 and proNGF appear to be products of granulosa cells; processing of proNGF to NGF appears to regulate apoptosis. PMID: 26457789
    40. The multivariate stepwise logistic regression identified the following biomarkers as the best gastric cancer predictors: CEA, CA72-4, pepsinogen I, Helicobacter pylori presence and MMP7 PMID: 27069188
    41. The ELM7 assay was specific towards in vitro MMP-7 degraded elastin. PMID: 26164539
    42. Study shows that MiR-543 inhibits translation of MMP7 through binding to the 3'-UTR of MMP7 mRNA in ovarian cancer. PLGF suppresses miR-543, which activates MMP7-mediated cancer invasion. PMID: 26402225
    43. Protein, mRNA, and serum expression levels of MMP-7 and IL-15 in patients with osteoarthritis were all significantly increased in osteoarthritis patients compared with the control group. PMID: 26464654
    44. Data showed that AEG1 and MMP7 levels were both significantly increased and strongly correlated in non-small cell lung cancer (NSCLC) tissues. AEG-1 promotes NSCLC cell invasiveness through MAPK-p42/p44-dependent activation of MMP7. PMID: 26418251
    45. For the MMP7 -181 A/G polymorphism, a decreased bladder cancer risk was found (G-allele vs. A-allele. PMID: 26301605
    46. In a Chinese population, genetic polymorphisms in MMP7 were associated with risk of coal workers pneumoconiosis. PMID: 26330178
    47. Data show that 5-hydroxytryptamine receptor (5-HT1DR) played an important role in cell invasion via Axin1/beta-catenin/matrix metalloproteinase 7 (MMP-7) pathway. PMID: 26214021
    48. Urinary MMP7 improves the overall diagnostic performance of urinary CXCL10 for distinguishing normal histology from subclinical and clinical inflammation/injury, but not subclinical inflammation alone. PMID: 26906940
    49. Our data suggested a potential role of Rab11-FIP2 in tumor progression and provided novel insights into the mechanism of how Rab11-FIP2 positively regulated cell migration and invasion in CRC cells. PMID: 26792722
    50. MMP-7 expression is highly specific, though only of moderate sensitivity, for the diagnosis of carcinoma in the differential diagnosis from both benign and malignant mesothelial cells. PMID: 26520416

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  • 亞細(xì)胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    Peptidase M10A family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 7174

    OMIM: 178990

    KEGG: hsa:4316

    STRING: 9606.ENSP00000260227

    UniGene: Hs.2256