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中文名稱:人基質(zhì)金屬蛋白酶8/中性粒細(xì)胞膠原酶(MMP-8)酶聯(lián)免疫試劑盒
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貨號:CSB-E04680h
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人基質(zhì)金屬蛋白酶8/中性粒細(xì)胞膠原酶(MMP-8)酶聯(lián)免疫試劑盒(CSB-E04680h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MMP8含量。MMP8,即基質(zhì)金屬蛋白酶8,是一種降解細(xì)胞外基質(zhì)(ECM)的酶,參與多種生理和病理過程。研究發(fā)現(xiàn),MMP8在壓力易感性和抑郁癥中發(fā)揮重要作用,其表達增加與應(yīng)激相關(guān)神經(jīng)精神疾病的發(fā)生發(fā)展密切相關(guān)。研究機制表明,MMP8通過影響神經(jīng)元功能和行為,以及改變細(xì)胞外空間,參與應(yīng)激相關(guān)障礙的發(fā)生。試劑盒檢測范圍為39.06 pg/mL-2500 pg/mL,靈敏度為9.77 pg/mL。適用于基礎(chǔ)研究領(lǐng)域,如炎癥性疾病機制探索、纖維化進程分析或腫瘤微環(huán)境中基質(zhì)重塑相關(guān)研究,為體外實驗提供可靠的蛋白表達水平數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:CLG 1 ELISA Kit; CLG1 ELISA Kit; Collagenase 1 ELISA Kit; Collagenase 1 neutrophil ELISA Kit; HNC ELISA Kit; Matrix metallopeptidase 8 (neutrophil collagenase) ELISA Kit; Matrix metalloprotease 8 ELISA Kit; Matrix metalloproteinase-8 ELISA Kit; MMP 8 ELISA Kit; MMP-8 ELISA Kit; Mmp8 ELISA Kit; MMP8_HUMAN ELISA Kit; Neutrophil collagenase ELISA Kit; PMNL CL ELISA Kit; PMNL collagenase ELISA Kit; PMNL-CL ELISA Kit; PMNLCL ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:39 pg/mL-2500 pg/mL
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靈敏度:9.75 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cancer
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
313.254
310.763
312.423
312.423
311.592
314.302
SD
0.043
0.048
0.041
0.052
0.057
0.054
CV(%)
4.489
5.042
4.289
5.439
5.975
5.619
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
94
Range %
86-99
1:3
Average %
96
Range %
91-101
1:9
Average %
92
Range %
89-95
1:27
Average %
94
Range %
88-98
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回收率:
The recovery of human MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
89-99
EDTA plasma (n=4)
95
90-99
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
2500
2.580
2.482
2.531
2.403
1250
2.152
2.092
2.122
1.994
625
1.613
1.588
1.601
1.473
312
0.930
0.920
0.925
0.797
156
0.502
0.492
0.497
0.369
78
0.347
0.337
0.342
0.214
39
0.222
0.218
0.220
0.092
0
0.128
0.127
0.128
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- The Role of Beta-Defensin 2 in Preventing Preterm Birth with Chorioamnionitis: Insights into Inflammatory Responses and Epithelial Barrier Protection S Yun, SH Kang, J Ryu, K Kim, KY Lee, JJ Lee,International Journal of Molecular Sciences,2025
- The Predictive Value of Serum DAO, HDC, and MMP8 for the Gastrointestinal Injury in the Early Stage of Acute Pancreatitis in an Animal Model and a Clinical Study R Cheng,/,2024
- Neuropeptide Y, a potential marker for lupus, promotes lupus development DC Wang,International immunopharmacology,2023
- Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown Braber et al,AJP - Lung Physiol,2011
- MMPs and myeloperoxidase in GCF provide site-specific diagnostic value for chronic periodontitis Leppilahti J et al,J Clin Periodontol,2013
相關(guān)產(chǎn)品
靶點詳情
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最新研究進展:MMP8,全名為基質(zhì)金屬蛋白酶-8,也稱為中性粒細(xì)胞膠原酶,是一種參與膠原蛋白降解的酶類分子。最近的研究表明,MMP8在多種炎癥和感染性疾病的發(fā)生和發(fā)展中發(fā)揮著重要作用,同時也參與癌癥、關(guān)節(jié)炎等多種疾病的發(fā)生和發(fā)展。
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功能:Can degrade fibrillar type I, II, and III collagens.
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基因功能參考文獻:
- The meta-analysis results showed a remarkable association between rs11225394 in MMP-8 gene and an increased risk of osteonecrosis of the femoral head and a significant association between MMP-8 rs2012390 and the decreased risk of osteonecrosis of the femoral head. PMID: 30313082
- Results suggest that MMP8 C-799T, Val436Ala and Lys460Thr may only play an indirect role in determining personal cancer susceptibility for bladder cancer in Taiwan. PMID: 30194163
- Low MMP8 level is associated with gastric cancer. PMID: 30192205
- Five single nucleotide polymorphisms including rs3740938, rs2012390, rs1940475, rs11225394, and rs11225395 of MMP-8 gene were genotyped in a Chinese Han population. It was found rs3740938 of MMP-8 was associated with an increased risk of ankylosing spondylitis under the dominant model and additive model after adjustment for gender and age by performing logistic regression analysis. PMID: 30170451
- Data show that polymorphism in the promoter region of matrix metallopeptidase 8 (MMP-8) (-799C/T) and two non-synonymous polymorphisms (Val436Ala and Lys460Thr) were not significantly associated with risk of pterygium.pterygium. PMID: 29275297
- Persistent oral human papillomavirus infection was associated with a low salivary MMP-8 concentration indicating eventually a failure in oral anti-inflammatory defence. PMID: 29078079
- Genetic polymorphism in S100A9-S100A12-S100A8 locus affects serum and plasma MMP-8 and shows a suggestive association with the risk of cardiovascular diseases. PMID: 29212897
- Our study demonstrated that the MMP-8 C-799T is associated with the risk of developing severe preeclampsia during pregnancy. However, the MMP-8 C + 17G polymorphism might not be a risk factor for susceptibility to preeclampsia. PMID: 28745526
- Our findings suggest that the polymorphisms at MMP-8 -799C/T, Val436Ala and Lys460Thr may not play a major role in determining the personal susceptibility to childhood acute lymphoblastic leukemia in Taiwan. PMID: 29102926
- Reduction in serum levels of MMP8 predicted complete remission in type 2 diabetes mellitus patients following bariatric surgery. PMID: 28734574
- MMP8 -799C/T, Val436Ala and Lys460Thr polymorphisms may only play an indirect role in determining personal cancer susceptibility to breast cancer in Taiwan. PMID: 29599337
- Our findings suggest that the polymorphisms at MMP-8 C-799T or Val436Ala may not play a major role in mediating personal risk of oral cancer; however, the detailed mechanisms require further investigation. PMID: 28652424
- Suggest that Ang-(1-7) plays an important role in protecting against atherosclerosis via counter-regulation of Ang II-induced MMP-8. PMID: 28283184
- Data show that individuals with MMP-8 -799TT genotype and intermediate HIV disease stage are at higher risk for the advancement of HIV disease. MMP-8 polymorphism indicated a trend of elevated risk for the modulation of HIV-associated neurocognitive disorder (HAND) severity. MMP-8 -799TT genotype may facilitate the risk for the development of HAND with tobacco and alcohol usage. PMID: 29292194
- Our study provides evidence for the tumour-suppressive mechanisms of MMP-8 in OTSCC by interplay with TGF-b1 and VEGF-C. PMID: 28772283
- These results provide the first evidence that MMP8 SNP at the rs11225394 locus is associated with the increased risk of osteonecrosis of the femoral head in Chinese Han population. PMID: 28423488
- Low MMP-8/TIMP-1 reflects left ventricle impairment in takotsubo cardiomyopathy and high TIMP-1 may help to differentiate it from acute coronary syndrome PMID: 28278213
- there is a negative correlation between blood MMP8 and HDL-cholesterol levels, suggesting a contributory role of MMP8 in metabolic alterations in acne inversa PMID: 27843200
- MMP-8 is a vital component of the myoepithelial tumour-suppressor function. It restores MEC interaction with the matrix, opposes TGF-beta signalling and MMP-9 proteolysis, which contributes to inhibition of tumour cell invasion. PMID: 28330493
- a 7-gene signature was identified which correctly predicted the primary prefibrotic myelofibrosis group with a sensitivity of 100% and a specificity of 89%. The 7 genes included MPO, CEACAM8, CRISP3, MS4A3, CEACAM6, HEMGN, and MMP8 PMID: 27579896
- Obesity is associated with elevated circulating MMP-8 in young adults. MMP-8 is also increased in smokers. PMID: 27296149
- Serum level patterns of MMP-2 and MMP-8 showed distinctive patterns for patients with spinal cord injury neurological impairment. MMP-8 and MMP-9 patterns showed significant differences regarding functional recovery. Our binary logistic regression model showed that, according to neurological damage, measuring peripheral serum levels can be used to monitor and predict locomotor recovery after spinal cord injury. PMID: 27377304
- rs1940475 and rs11225395 associated with 1.32-fold increased risk of steroid-induced osteonecrosis of the femoral head PMID: 27631232
- Blood expression of matrix metalloproteinases 8 and 9 and of their inducers S100A8 and S100A9 supports diagnosis and prognosis of PDAC-associated diabetes mellitus PMID: 26923392
- Plasma MMP-8 and MMP-9 concentrations correlate with diabetic ketoacidosis severity and are known to degrade brain microvascular endothelial cell tight junctions. Thus, leukocyte-derived MMPs might contribute to DKA-associated cerebrovascular complications. PMID: 26492282
- Initial analysis of the MMP8 gene showed suggestive association between rs1940475 and knee OA, but the finding did not replicate in other study cohorts, even though the trend for predisposing allele was similar in all five cohorts. MMP-8 is a good biological candidate for OA, but our study did not find common variants with significant association in the gene. PMID: 26577236
- Sputum, serum, and urine MMP-8 were not significantly changed in COPD exacerbation compared to recovery values. PMID: 26418236
- amniotic fluid concentrations in acute-chorioamnionitis distinctly decrease throughout preterm-gestation PMID: 26462905
- Suggest that MMP-8 polymorphism -799 C/T was a risk for developing chronic periapical lesions. PMID: 27442388
- The reciprocal positive interplay between MMP-8 and TGF-beta1 contributes to HCC invasion and metastasis by inducing EMT mainly through the PI3K/Akt/Rac1 pathway. PMID: 26872724
- increased levels in saliva and serum in women with polycystic ovary syndrome, and is potentiated in the presence of gingivitis PMID: 25712810
- Positive results of the aMMP-8 test significantly correlate with generalized ChP. The aMMP-8 test may be used by physicians to detect periodontitis in their patients. PMID: 25841875
- miR-539 plays a key role in inhibiting osteosarcoma cell invasion and migration and can regulate MMP8 expression in osteosarcoma cells. PMID: 26339374
- salivary levels of the analyzed biomarkers MMP-8, -9, MPO are associated with periodontal status. However, these biomarkers could not differentiate between patients with or without a MI. PMID: 26132583
- Moderate-strength STS causes the highest TIMP-1/MMP-8 ratio, leading to appropriate conditions for reformation of the extracellular matrix PMID: 23851938
- Gender-specific analysis of MMPs demonstrated consistent increase in MMP-1 and -8 in tuberculosis, but MMP-8 was a better discriminator for TB in men. PMID: 25635689
- MMP-8, MMP-9, and YKL-40 might serve as novel non-invasive biomarkers of CF lung disease and pulmonary exacerbations. PMID: 25545245
- Low levels of plasma MMP8 levels can rule out acute aortic dissection. PMID: 23442769
- investigated whether MMP-8 affects the structure and antiatherogenic function of apolipoprotein (apo) A-I, the main protein component of HDL particles PMID: 25550459
- Strong associations of MMP-8 with components of Metabolic Syndrome X in univariate, bivariate and multivariate models suggest plasma MMP-8 as a potential cardiometabolic risk marker for Metabolic syndrome X. PMID: 25633268
- it can be suggested that MMP-8 -799 C/T and TIMP-1 372 T/C, *429 T/G gene polymorphisms in males may be associated with the susceptibility to GAgP in the Turkish population. PMID: 24283658
- Studied plasma MMP-8 levels and its correlates 20+/-3 months after acute myocardial infarction. PMID: 24164993
- The 799C/T polymorphism in the promoter region of MMP8 may be associated with the development of TAD and that the T allele may increase patient predisposition to the disease. PMID: 25109362
- MMP-8 promoter gene polymorphism -799 T/T is significantly associated with an increased risk of ovarian cancer in Mexican women. PMID: 25034366
- patients with high serum MMP-8 levels may benefit from adjuvant IFN-alpha therapy, but this observation should be further investigated. PMID: 25319807
- Plasma and BALF MMP-8 levels are unlikely to serve as a prognostic biomarker for IPF patients. PMID: 24828408
- MMP8 rs1940475 SNP modifies the host response to inflammatory stimuli. PMID: 24170307
- The polymorphism at position -799 of the gene for MMP-8 is associated with tendinopathy primary posterior tibial tendon in the population studied. The results suggest that individuals with the T allele are at greater risk of developing tendinopathy. PMID: 22487237
- Studied the plasma concentrations of MMP-8, TIMP1, C-reactive protein, fibrinogen, and WBCs in patients with acute coronary syndrome and found levels were significantly higher than those in the control group. PMID: 25016699
- Plasmatic OxLDL and MMP-8 levels are associated with carotid atherosclerosis PMID: 24267248
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亞細(xì)胞定位:Cytoplasmic granule. Secreted, extracellular space, extracellular matrix. Note=Stored in intracellular granules.
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蛋白家族:Peptidase M10A family
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組織特異性:Neutrophils.
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