Human Methemoglobin,MHB ELISA Kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人高鐵血紅蛋白(MHB)酶聯免疫試劑盒（CSB-E09493h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的MHB含量。試劑盒檢測范圍為0.47 μg/mL-30 μg/mL，高鐵血紅蛋白水平變化可為氧化應激相關機制研究提供參考。本產品適用于基礎醫(yī)學研究中探索溶血性貧血、藥物毒性反應、環(huán)境污染物暴露等場景下血紅蛋白氧化損傷的動態(tài)變化，也可用于體外實驗中評估化合物對紅細胞氧化還原狀態(tài)的影響，為氧化應激相關疾病機制研究及藥物開發(fā)中的毒性評估提供定量檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

MHB
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.47 μg/mL-30 μg/mL
靈敏度：

0.11 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	80-91
1:200	Average %	87
	Range %	83-93
1:400	Average %	90
	Range %	87-93
1:800	Average %	95
	Range %	91-103

回收率：

The recovery of human MHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	88	82-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.