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  4. Human Monoglyceride lipase(MGLL) ELISA kit

Human Monoglyceride lipase(MGLL) ELISA kit

  • 中文名稱：
    人單甘油脂酶(MGLL)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL013787HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人單甘油脂酶(MGLL)酶聯(lián)免疫試劑盒（CSB-EL013787HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的MGLL含量。MGLL是重要靶點(diǎn)。它在體內(nèi)參與內(nèi)源性大麻素系統(tǒng)的代謝過(guò)程。研究發(fā)現(xiàn)其可能調(diào)節(jié)神經(jīng)信號(hào)傳導(dǎo)、炎癥反應(yīng)等生理機(jī)制。對(duì)其深入研究有助于了解相關(guān)疾病病理，有望為開(kāi)發(fā)神經(jīng)系統(tǒng)、炎癥性疾病等藥物提供新方向。試劑盒檢測(cè)范圍為39.06 pg/mL-2500 pg/mL，適用于基礎(chǔ)科研中探究MGLL在代謝調(diào)控網(wǎng)絡(luò)中的作用、評(píng)估疾病模型（如非酒精性脂肪肝、2型糖尿?。┲忻富钚宰兓?，或用于藥物干預(yù)實(shí)驗(yàn)的分子標(biāo)記物檢測(cè)，為脂代謝通路研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    EC 3.1.1.23 ELISA Kit; HU K5 ELISA Kit; HU-K5 ELISA Kit; HUK5 ELISA Kit; Lysophospholipase homolog ELISA Kit; Lysophospholipase like ELISA Kit; Lysophospholipase-like ELISA Kit; MAGL ELISA Kit; MGL ELISA Kit; MGLL ELISA Kit; MGLL_HUMAN ELISA Kit; Monoacylglycerol lipase ELISA Kit; Monoglyceride lipase ELISA Kit
  • 縮寫(xiě)：
    MGLL
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    39.06 pg/mL-2500 pg/mL
  • 靈敏度：
    9.76 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MGLL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %91-99
    1:2Average %104
    Range %101-107
    1:4Average %97
    Range %92-102
    1:8Average %98
    Range %93-104
  • 回收率：
    The recovery of human MGLL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8782-91
    EDTA plasma (n=4)9491-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    25002.524 2.537 2.531 2.370
    12501.661 1.687 1.674 1.513
    6251.076 1.082 1.079 0.918
    312.50.734 0.704 0.719 0.558
    156.250.511 0.504 0.508 0.347
    78.130.375 0.392 0.384 0.223
    39.060.302 0.298 0.300 0.139
    00.165 0.157 0.161
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
  • 基因功能參考文獻(xiàn)：
    1. Using a multimethod approach, the authors show that the dynamically relevant Trp-289 and Leu-232 residues serve as communication hubs within an allosteric protein network that controls signal propagation to the active site, and thus, regulates active-inactive interconversion of human MGL. PMID: 29379013
    2. RNA interference, specific pharmacological inhibitor JZL-184 and gene knock-in of MAGL were utilized to investigate the effects of MAGL on hepatocellular carcinoma (HCC) cell proliferation, apoptosis, and invasion. MAGL played important roles in both proliferation and invasion of HCC cells. PMID: 27767105
    3. This review summarizes the basics of monoglyceride metabolism and provides an overview on the therapeutic potential of MGL. [Review] PMID: 28213089
    4. MGLL may have a role in progression of gastrointestinal stromal tumors PMID: 27366945
    5. the upregulation of MAGL in hepatocellular carcinoma cells promoted cell growth and invasiveness abilities, and mediated epithelial-mesenchymal transition PMID: 27884159
    6. we clarify the key role of Phe159 and Ile179, two conserved residues within the lid domain, in regulating substrate specificity in MAGL. We conclude by proposing that other structurally related lipases may share this lid-domain-mediated mechanism for substrate specificity. PMID: 28088576
    7. the presence and differential distribution of fatty acid amide hydrolase (FAAH) and monoglyceride lipase (MGLL) in relation to CB1 during the maturation of human oocytes, was investigated. PMID: 26948343
    8. there was evidence that MGLL rs604300 genotype interacts with early life adversity to predict threat-related basolateral amygdala habituation, a neural phenotype linked to the endocannabinoid system and addiction PMID: 26595473
    9. This study unravels a novel mechanism of SND1 function and identifies MGLL as a unique tumor suppressor for HCC. MGLL might function as a homeostatic regulator of Akt restraining its activation. PMID: 26997225
    10. The data highlight specific inter-residue interactions within hMGL PMID: 26555264
    11. Monoacylglycerol lipase sulfenylation might act as an intrinsic neuroprotective mechanism by potentiating 2-AG signaling at CB1 receptors. PMID: 26000748
    12. Molecular dynamics and nudged elastic band simulations were used to explore the conformational transition pathway of the helix alpha4 of human monoacylglycerol lipase. PMID: 25078047
    13. role of monoacylglycerol lipase (MAGL) in the cancer progress PMID: 24633487
    14. Our findings establish that MAGL promotes metastases in nasopharyngeal carcinoma PMID: 25120746
    15. In subcutaneous adipose tissue, DAGL-a mRNA was upregulated and fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) mRNAs were down-regulated in obese subjects, but the diets had no influence. PMID: 24616451
    16. In obese humans, FAAH or MGL activity in adipocytes is not affected by diabetes, dyslipidaemia or other markers of metabolic dysfunction. PMID: 24593280
    17. Mutation of Cys242 was also found to impair inhibition of monoacylglycerol lipase. PMID: 24368842
    18. MGL interaction with a phospholipid membrane bilayer induces regional changes in the enzyme's conformation that favor its recruiting lipophilic substrate from membrane stores to the active site resulting in enhanced MGL catalytic activity. PMID: 23553709
    19. Data indicate that N,N-dimethyl-5-(4-phenoxyphenyl)-2H-tetrazole-2-carboxamide inhibited monoacylglycerol lipase (MAGL) with IC50 0.028 muM. PMID: 23455058
    20. MGL expression declines after peaking in infancy. PMID: 22827915
    21. results suggest that MGL plays a negative regulatory role in phosphatidylinositol-3 kinase/Akt signaling and tumor cell growth PMID: 22349814
    22. No association was found between our inflammatory bowel disease cohort and the candidate single nucleotide polymorphisms for MGL (CD/HC: P=0.37 and UC/HC: P=0.25). PMID: 22664939
    23. MAGL is elevated in androgen-independent versus androgen-dependent human prostate cancer cell lines, and pharmacological or RNA-interference disruption of this enzyme impairs prostate cancer aggressiveness. PMID: 21802006
    24. High MAGL is associated with colorectal cancer. PMID: 21543155
    25. Data present a high-resolution structure of a ligand-bound, soluble form of human monoglyceride lipase. PMID: 21308848
    26. One interval in the FAAH promoter and three intervals in the MGLL gene were associated with high BMI. PMID: 21118518
    27. Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid in the brain and is believed to be hydrolyzed primarily by serine hydrolase monoacylglycerol lipase (MAGL) in a transgenic mouse model with targeted disruption of MAGL. PMID: 20855465
    28. Identification of an active site hydrogen bond network in human monoacylglycerol lipase. PMID: 20464001
    29. An apolar helix covering the active site also gives structural insight into the amphitropic character of MAGL, and likely explains how MAGL interacts with membranes to recruit its substrate. PMID: 19957260
    30. MGL shares the classic fold of the alpha/beta hydrolase family but depicts an unusually large hydrophobic occluded tunnel with a highly flexible lid at its entry and the catalytic triad buried at its end. PMID: 19962385
    31. Study shows that monoacylglycerol lipase (MAGL) is highly expressed in aggressive cancer cells and primary tumors; it regulates a fatty acid network enriched in oncogenic signaling lipids that promotes migration, invasion, survival, and tumor growth. PMID: 20079333
    32. not associated with susceptibility to alcoholism in a Japanese population. PMID: 17621164
    33. full proteomic characterization of hMGL was carried out, which showed (1) an absence of intramolecular disulfide bridges in the functional, recombinant enzyme and (2) the post-translational removal of the enzyme's N-terminal methionine PMID: 18452279
    34. Identification of amino acids critical to the catalytic activity PMID: 18721756
    35. Gene Ontology analysis indicated a significant alteration of oxygen transport (increased hemoglobin gene expression) and lipid metabolism [including monoglyceride lipase and low density lipoprotein receptor-related protein 5 (LRP5) gene]. PMID: 19233690

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  • 亞細(xì)胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    AB hydrolase superfamily, Monoacylglycerol lipase family
  • 組織特異性：
    Detected in adipose tissue, lung, liver, kidney, brain and heart.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 17038

    OMIM: 609699

    KEGG: hsa:11343

    STRING: 9606.ENSP00000265052

    UniGene: Hs.277035