Human Myosin-11(MYH11) ELISA kit
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中文名稱:人肌球蛋白-11(MYH11)酶聯(lián)免疫試劑盒
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貨號:CSB-EL015291HU
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規(guī)格:96T/48T
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價格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人肌球蛋白-11(MYH11)酶聯(lián)免疫試劑盒(CSB-EL015291HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的MYH11含量。MYH11是重要的靶點。它是一種肌球蛋白重鏈,參與平滑肌收縮等生理過程。其異常表達(dá)或功能改變與多種疾病相關(guān),如血管疾病等。研究機(jī)制多聚焦于其在疾病發(fā)生發(fā)展中的信號通路作用,以及與其他分子的相互作用,以探索潛在治療策略。試劑盒檢測范圍為25 pg/mL-1600 pg/mL,適用于科研領(lǐng)域中對平滑肌相關(guān)疾病機(jī)制、腫瘤微環(huán)境調(diào)控或血管功能研究中的MYH11蛋白表達(dá)水平檢測,為體外實驗提供穩(wěn)定可靠的數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:AAT4 ELISA Kit; DKFZp686D10126 ELISA Kit; DKFZp686D19237 ELISA Kit; FAA4 ELISA Kit; FLJ35232 ELISA Kit; MGC126726 ELISA Kit; MGC32963 ELISA Kit; MYH 11 ELISA Kit; MYH11 ELISA Kit; MYH11_HUMAN ELISA Kit; Myosin 11 ELISA Kit; Myosin heavy chain 11 ELISA Kit; Myosin heavy chain 11 smooth muscle ELISA Kit; Myosin heavy chain ELISA Kit; Myosin heavy chain smooth muscle isoform ELISA Kit; Myosin heavy polypeptide 11 smooth muscle ELISA Kit; Myosin-11 ELISA Kit; SMHC ELISA Kit; SMMHC ELISA Kit; smooth muscle isoform ELISA Kit; Smooth muscle myosin heavy chain 11 isoform SM2 ELISA Kit; Smooth muscle myosin heavy chain isoform SM2 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:25 pg/mL-1600 pg/mL
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靈敏度:6.25 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human MYH11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 89-99 1:2 Average % 102 Range % 97-105 1:4 Average % 98 Range % 92-101 1:8 Average % 95 Range % 86-98 -
回收率:
The recovery of human MYH11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 94-101 EDTA plasma (n=4) 88 82-94 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1600 2.295 2.498 2.397 2.258 800 1.543 1.442 1.493 1.354 400 0.891 0.843 0.867 0.728 200 0.528 0.537 0.533 0.394 100 0.322 0.335 0.329 0.190 50 0.255 0.267 0.261 0.122 25 0.174 0.179 0.177 0.038 0 0.138 0.139 0.139 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- BLOOD BIOMARKER FOR DETECTING ARTERIOSCLEROSIS Yokoyama, et al,/,2019
- Proteomic analysis of aortic smooth muscle cell secretions reveals an association of myosin heavy chain 11 with abdominal aortic aneurysm Utako Yokoyama.et al,American Journal of Physiology-Heart and Circulatory Physiology,2018
相關(guān)產(chǎn)品
靶點詳情
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功能:Muscle contraction.
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基因功能參考文獻(xiàn):
- the presented study demonstrates that CBFB-MYH11-based MRD status during the first 3 months after allo-HCT, but not KIT mutations, can be used to identify patients with a high risk of relapse. PMID: 27650511
- In patients with MYH11 or ACTA2 variants, the effect of intronic variants on splicing was demonstrated on the mRNA level in the induced smooth muscle cell (SMC), allowing classification into pathogenic or nonpathogenic variants. PMID: 28074631
- Deletion mutation in MYH11 gene causing familial Thoracic aortic dissection was identified in two independent Japanese pedigrees. PMID: 26056961
- Data suggest that expression of MYH11, myosin light chain, and MLCK (myosin-light-chain kinase), is up-regulated in uterine myoma as compared to adjacent smooth muscle cells; expression of MYH11 appears to be involved in cell proliferation. PMID: 25181625
- In familial AAA we found one pathogenic and segregating variant (COL3A1 p.Arg491X), one likely pathogenic and segregating (MYH11 p.Arg254Cys), and fifteen VUS. PMID: 26017485
- CBFB contributes to the transcriptional regulation of ribosomal gene expression and provide further understanding of the epigenetic role of CBFB-SMMHC in proliferation and maintenance of the leukemic phenotype. PMID: 25079347
- we report a novel hypomethylation pattern, specific to CBFB-MYH11 fusion resulting from inv(16) rearrangement in acute myeloid leukemia the expression of which correlated with PBX3 differential methylation PMID: 25266220
- overexpression of MYH11 can lead to increased ER stress and autophagy PMID: 24711452
- MYH11 gene mutation is associated with family history of thoracic aortic aneurysm dissection. PMID: 24921172
- Transcriptional analysis revealed that upon fusion protein knockdown, a small subset of the CBFbeta-MYH11 target genes show increased expression, confirming a role in transcriptional repression PMID: 24002588
- MYH11 mutations are rare and are identified in patients with thoracic aortic aneurysm/dissection. PMID: 21937134
- Incomplete segregation of MYH11 variants with thoracic aortic aneurysms and dissections and patent ductus arteriosus. PMID: 22968129
- We conclude that non-type A CBFB-MYH11 fusion types associate with distinct clinical and genetic features, including lack of KIT mutations, and a unique gene-expression profile in acute myeloid leukemia PMID: 23160462
- Our data indicate that the CBFbeta-SMMHC's C-terminus is essential to induce embryonic hematopoietic defects and leukemogenesis. PMID: 23152542
- A rare variant in MYH11, R247C, alters myosin contractile function and smooth muscle cell phenotype, leading to increased proliferation in vitro and in response to vascular injury. PMID: 22511748
- Data show that homozygous and compound heterozygous changes found in PLOD1 and SLC2A10 may confer autosomal recessive effects, and three MYH11, ACTA2 and COL3A1 heterozygous variants were considered as putative pathogenic gene alterations. PMID: 22001912
- increased MYH11 expression was found in aortic tissues from TAAD patients with 16p13.1 duplications compared with control aortas. PMID: 21698135
- Data show that the purified hMDCs cultured in SMIM for 4 weeks and expressed significant amount of smooth muscle myosin heavy chain and alpha-smooth muscle actin. PMID: 20132408
- Data suggest that that hASMCs contain a significant pool of functional SMM in the 10S conformation that can assemble into filaments upon changing cellular conditions. PMID: 21205888
- CBFB-MYH11 rearrangement is associated with acute myeloid leukemia. PMID: 20508610
- leukemogenic fusion gene (with Cbfb) plays a role in hematopoiesis PMID: 12239155
- Plag1 and Plagl2 are novel leukemia oncogenes that act by expanding hematopoietic progenitors expressing CbF beta-SMMHC. PMID: 15585652
- Human MYH11 gene mutations provide the first example of a direct change in a specific smooth muscle cell protein leading to an inherited arterial diseases PMID: 16444274
- Detection of acute myeloid leukemic cells that are characterized by a CBFB-MYH11 gene fusion. PMID: 16502584
- These observations suggest that when abdominal GS is diagnosed, an analysis of the CBFB/MYH11 fusion gene is necessary to make an appropriate decision regarding treatment options, even if no chromosomal abnormalities are found. PMID: 16504290
- Agents interacting with the outer surface of the CBFbeta-SMMHC ACD that prevent multimerization may be effective as novel therapeutics in AML PMID: 16767164
- Rare fusion transcripts were correlated with an atypical cytomorphology not primarily suggestive for the FAB subtype acute myelocytic leukemia. PMID: 17287858
- Examine consequences of expression of abnormal chimeric protein CBFbeta-MYH11 in acute myelomonocytic leukemia. PMID: 17571080
- MYH11 mutations are likely to be specific to the phenotype of thoracic aortic aneurysms and dissections associated with patent ductus arteriosus and result in a distinct aortic and occlusive vascular pathology potentially driven by IGF-1 and Ang II. PMID: 17666408
- MYH11 gene is involved in only rare instances when persistent patency of the arterial duct occurs in sporadic fashion. PMID: 17956658
- MYH11 mutations in patients with colorectal cancer, Peutz-Jeghers syndrome and juvenile polyposis . PMID: 18391202
- Little evidence for a role of somatic MYH11 mutations in the formation of breast or prostate cancers PMID: 18796164
- three novel amino acid substitutions in MYH11 in AML samples, located in the highly conserved myosin head and rod essential for motor function and regulation of MYH11 PMID: 18798114
- MYH11 mutation is not required for early hereditary nonpolyposis colorectal cancer adenoma formation, but it is selected for in the process of microsattelite instability positive cancer tumorigenesis. PMID: 18941465
- Selective overexpression of airway smooth muscle genes in asthmatic airways leads to increased Vmax, thus contributing to the airway hyperresponsiveness observed in asthma. PMID: 19011151
- sequence deletion in Pseudoxanthoma elasticum PMID: 11439001
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相關(guān)疾病:Aortic aneurysm, familial thoracic 4 (AAT4)
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亞細(xì)胞定位:Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
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蛋白家族:TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
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組織特異性:Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles.
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