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  4. Human N-terminal procollagen Ⅳ propeptide,PⅣNP ELISA Kit

Human N-terminal procollagen Ⅳ propeptide,PⅣNP ELISA Kit

  • 中文名稱：
    人Ⅳ型前膠原氨基末端肽(PⅣNP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EQ027190HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人Ⅳ型前膠原氨基末端肽(PⅣNP)酶聯(lián)免疫試劑盒（CSB-EQ027190HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PⅣNP含量。試劑盒檢測范圍為2.8 ng/mL-180 ng/mL，常用于肝纖維化、肺纖維化等疾病模型的機制研究，適用于體外培養(yǎng)體系、動物實驗樣本中膠原合成活性的動態(tài)監(jiān)測，可為纖維化相關(guān)分子機制探索、藥物干預(yù)效果評估等研究提供可靠數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N-terminal procollagen 4 propeptide,P4NP,_x000D_ N-terminal procollagen IV propeptide,PIVNP
  • 縮寫：
    PⅣNP
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    2.8 ng/mL-180 ng/mL
  • 靈敏度：
    0.7 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PIVNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:5 Average % 99    
    Range % 87-104    
    1:10 Average % 105    
    Range % 100-110    
    1:20 Average % 93    
    Range % 86-97    
    1:40 Average % 104    
    Range % 95-107    
  • 回收率：
    The recovery of human PIVNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 107 100-111    
    EDTA plasma (n=4) 101 95-107    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    180 2.625 2.544 2.585 2.393    
    90 2.300 2.266 2.283 2.091    
    45 1.710 1.743 1.727 1.535    
    22.5 1.144 1.102 1.123 0.931    
    11.2 0.690 0.655 0.673 0.481    
    5.6 0.489 0.467 0.478 0.286    
    2.8 0.314 0.302 0.308 0.116    
    0 0.196 0.188 0.192 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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