Human NGAL/MMP-9 complex ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人NGAL/MMP-9復(fù)合物酶聯(lián)免疫試劑盒（CSB-EQ01026975HU）檢測(cè)血清、血漿、唾液、組織培養(yǎng)上清液、組織勻漿樣本中的NGAL/MMP-9 complex含量。NGAL/MMP-9 complex 作為靶點(diǎn)備受關(guān)注。它在多種疾病如腫瘤、炎癥等病理過程中發(fā)揮重要作用。研究機(jī)制上，二者形成復(fù)合物后可影響細(xì)胞生長(zhǎng)、遷移、侵襲等行為，調(diào)節(jié)組織的微環(huán)境，通過干擾其相互作用有望為相關(guān)疾病治療提供新思路。試劑盒檢測(cè)范圍為0.313 ng/mL-20 ng/mL，靈敏度為0.029 ng/mL。適用于基礎(chǔ)醫(yī)學(xué)研究中疾病機(jī)制探索、生物標(biāo)志物分析及藥物干預(yù)效果評(píng)估。為炎癥相關(guān)疾病模型構(gòu)建、腫瘤微環(huán)境研究及腎臟功能損傷實(shí)驗(yàn)提供高效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

NGAL/MMP-9 complex
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, saliva, cell culture supernates, tissue homogenates
檢測(cè)范圍：

0.313 ng/mL-20 ng/mL
靈敏度：

0.029 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL/MMP-9 complex in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	91
Range %	87-98
1:20	Average %	95
Range %	90-102
1:40	Average %	93
Range %	87-99
1:80	Average %	94
Range %	86-105

回收率：

The recovery of human NGAL/MMP-9 complex spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	94	87-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.