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  4. Human Napsin-A(NAPSA) ELISA kit

Human Napsin-A(NAPSA) ELISA kit

  • 中文名稱：
    人天冬氨酸蛋白酶A(NAPSA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL015452HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人天冬氨酸蛋白酶A(NAPSA)酶聯(lián)免疫試劑盒（CSB-EL015452HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NAPSA含量。人天冬氨酸蛋白酶A（NAPSA）是一種在細胞凋亡和腫瘤發(fā)生中發(fā)揮關鍵作用的蛋白酶。其研究機制涉及其在細胞內的相互作用、催化活性以及與底物的特異性結合。NAPSA通過切割特定肽鍵來調控細胞進程，其抑制劑作為潛在治療劑備受關注。試劑盒檢測范圍為4.7 ng/mL-300 ng/mL，科研人員可利用定量分析體液或組織樣本中的NAPSA濃度變化，應用于肺發(fā)育生物學研究、腫瘤微環(huán)境分析、疾病機制探索等領域，為研究該蛋白在細胞分化、組織修復及病理狀態(tài)下的調控作用提供實驗工具。試劑盒操作流程標準化，配套實驗方案詳實，適用于生命科學基礎研究與生物標志物探索等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    Asp 4 ELISA Kit; ASP4 ELISA Kit; Aspartyl protease 4 ELISA Kit; KAP ELISA Kit; Kdap ELISA Kit; Kidney derived aspartic protease like protein ELISA Kit; NAP1 ELISA Kit; NAPA ELISA Kit; Napsa ELISA Kit; NAPSA_HUMAN ELISA Kit; Napsin 1 ELISA Kit; napsin A aspartic peptidase ELISA Kit; Napsin A precursor ELISA Kit; Napsin-1 ELISA Kit; Napsin-A ELISA Kit; Pronapsin A ELISA Kit; SNAPA ELISA Kit; TA01/TA02 ELISA Kit
  • 縮寫：
    NAPSA
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    4.7 ng/mL-300 ng/mL
  • 靈敏度：
    1.18 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NAPSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:5 Average % 104
    Range % 97-108
    1:10 Average % 101
    Range % 95-104
    1:20 Average % 97
    Range % 89-101
    1:40 Average % 106
    Range % 98-110
  • 回收率：
    The recovery of human NAPSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 95-104
    EDTA plasma (n=4) 101 94-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    300 2.435 2.527 2.481 2.289
    150 2.042 2.104 2.073 1.881
    75 1.455 1.409 1.432 1.240
    37.5 0.843 0.823 0.833 0.641
    18.75 0.552 0.538 0.545 0.353
    9.4 0.381 0.376 0.379 0.187
    4.7 0.301 0.311 0.306 0.114
    0 0.190 0.194 0.192 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    May be involved in processing of pneumocyte surfactant precursors.
  • 基因功能參考文獻：
    1. These data indicated that napsin A expression may inhibit TGFbetalinduced EMT and was negatively associated with EMTmediated erlotinib resistance, suggesting that napsin A expression may improve the sensitivity of lung cancer cells to EGFRTKI through the inhibition of EMT. PMID: 29845258
    2. Although napsin A is infrequently expressed in endometrial carcinomas, positive results of napsin A immunostaining in endometrial neoplasms might support the diagnosis of clear cell carcinoma when the pathologic differential diagnosis includes other histologic subtypes PMID: 26945446
    3. it may be useful to combine NAPA and TTF-1 for increased sensitivity in lung cancer diagnostics. There is no substantial difference between monoclonal and polyclonal p40 and between different NAPA clones, whereas there is a difference between the TTF-1 clones 8G7G3/1 and SPT24 PMID: 26447895
    4. To the best of our knowledge, expression of monoclonal Napsin A in lymphomas has never been reported. ALK-DLBCL should be considered in the differential diagnosis when evaluating a Napsin A-positive tumor of poorly differentiated morphology and of unknown primary PMID: 26808134
    5. It is important for pathologists to be aware that breast carcinomas with apocrine features can express napsin A. PMID: 26842346
    6. High Napsin A expression is associated with adenocarcinoma in non-small cell lung carcinoma. PMID: 27412420
    7. Napsin-A, and Desmocollin-3 were sensitive and specific markers for the diagnosis of AC and SCC, respectively. Both markers allowed classification of 54/60 cases into either AC or SCC. PMID: 26710975
    8. napsin A is aberrantly expressed in a subset of lymphomas PMID: 26400099
    9. CK7, TTF-1 and napsin A are predominantly expressed in primary lung adenocarcinoma patients, with CDX-2 being inconsistently expressed. PMID: 26469326
    10. Combining HNF-1beta and napsin A may distinguish clear cell carcinoma from high-grade serous carcinoma, endometrioid adenocarcinoma and metastatic Krukenberg tumors. PMID: 26339401
    11. napsin A is another sensitive and specific marker for distinguishing ovarian clear cell tumors (especially adenocarcinomas) from other ovarian tumors PMID: 24721826
    12. Napsin A is expressed in a broad spectrum of renal neoplasms. PMID: 25889632
    13. Low expression levels of NAPSA is associated with lung adenocarcinoma. PMID: 25982999
    14. In diagnosis of ovarian clear cell carcinoma, Napsin A is specific but of intermediate sensitivity. PMID: 25551297
    15. Napsin A is frequently expressed in ovarian and endometrial clear cell carcinomas. PMID: 25971546
    16. Data indicate that polyclonal but not monoclonal napsin A antibody has a virtually universal nonspecific labeling in mucinous adenocarcinomas of various sites. PMID: 25521803
    17. These data suggest that napsin A may be a useful marker for identifying metastatic adenocarcinomas of pulmonary origin PMID: 24479710
    18. Our study showed that napsin A is an extremely sensitive (100%) marker of ovarian clear cell carcinomas PMID: 25389337
    19. Napsin A as a marker of clear cell ovarian carcinoma. PMID: 24191930
    20. PAX2 and napsin A have high specificity but low sensitivity and only have limited value in the differential diagnosis of mesotheliomas and renal cell carcinomas PMID: 23503645
    21. TTF-1 is more sensitive than napsin for detection of lung sarcomatoid carcinoma, and no cases were positive for napsin but negative for TTF-1 PMID: 24331839
    22. Napsin A is a sensitive and specific biomarker of the clear cell histotype in endometrial carcinomas and accordingly may have diagnostic utility in their histotyping. PMID: 24145649
    23. 24 cases each of pulmonary and esophageal adenocarcinoma were stained with TTF-1, napsin A, CDX2, 34betaE12, N-cadherin, and IMP3 in an attempt to find an optimal panel for differentiation. IMP3, CDX2, and N-cadherin are superior to either TTF-1 or napsin A. PMID: 23899066
    24. Napsin-A seems to be a useful marker in classifying primary pulmonary neoplasm as adenocarcinomas. PMID: 22914608
    25. A minority of anaplastic and poorly differentiated micropapillary pattern thyroid carcinomas are napsin A positive. PMID: 23681073
    26. Napsin B was duplicated from napsin A during the early stages of primate evolution, and the subsequent loss of napsin B function during primate evolution reflected ongoing human-specific napsin B pseudogenization PMID: 23333608
    27. Mucin-producing neoplasms of the lung infrequently express napsin A, suggesting that immunohistochemical assessment of napsin A may have limited diagnostic usefulness for distinguishing primary and metastatic mucinous adenocarcinomas involving the lung. PMID: 23355200
    28. investigation of expression of NAPSA (a potential diagnostic marker) in lungs/respiratory mucosa of subjects with pulmonary sclerosing hemangioma PMID: 23194051
    29. a useful immunohistochemical marker for differentiation of lung squamous cell carcinoma and adenocarcinoma from other subtypes PMID: 22495379
    30. absence of napsin A was an independent prognostic factor for reduced survival time in lung adenocarcinoma PMID: 22418245
    31. Nap-A was more specific than thyroid transcription factor 1 for primary lung adenocarcinoma versus all tumors, excluding kidney, independent of tumor type PMID: 22288963
    32. None of the 90 squamous cell carcinomas of the lung exhibited napsin A positivity in the neoplastic cells; however, strong napsin A reactivity was observed in hyperplastic type II pneumocytes and in intra-alveolar macrophages entrapped within the tumor. PMID: 22198009
    33. napsin A is a useful marker that can assist in the diagnosis of both lung adenocarcinomas and renal cell carcinomas[review] PMID: 22156835
    34. in combination with thyroid transcription factor-1-positive immunostaining helps in differentiating primary lung adenocarcinoma from metastatic carcinoma in the lung PMID: 21464700
    35. Although TTF-1 had a higher sensitivity, napsin-A was useful as a surrogate marker when encountering a poorly differentiated lung adenocarcinoma. PMID: 20830690
    36. Pronapsin A gene expression in normal and malignant human lung and mononuclear blood cells PMID: 12151090
    37. Napsin is a promising marker for the diagnosis of primary lung adenocarcinoma. PMID: 12698189
    38. role in the N- and C-terminal processing of pro-surfactant protein-B in type-II pneumocytes PMID: 13129928
    39. NAPSA suppresses tumor growth independent of its catalytic activity. PMID: 18195689
    40. A general defect in napsin A or cathepsin H expression or activity was not the specific cause for abnormal surfactant accumulation in juvenile pulmonary alveolar proteinosis PMID: 18216060
    41. The combined use of napsin A and thyroid transcription factor-1 results in improved sensitivity and specificity for identifying pulmonary adenocarcinoma in primary lung tumors and in a metastatic setting. PMID: 19740516

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase A1 family
  • 組織特異性：
    Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 13395

    OMIM: 605631

    KEGG: hsa:9476

    STRING: 9606.ENSP00000253719

    UniGene: Hs.512843