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  4. Human Neogenin(NEO1) ELISA kit

Human Neogenin(NEO1) ELISA kit

  • 中文名稱：
    人再生蛋白(NEO1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL015712HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人再生蛋白(NEO1)酶聯(lián)免疫試劑盒（CSB-EL015712HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的NEO1含量。NEO1 即神經(jīng)纖毛蛋白 - 1，它屬于細(xì)胞表面受體，在胚胎發(fā)育、血管生成等生理過(guò)程中發(fā)揮重要作用。其研究機(jī)制主要聚焦在與配體相互作用，調(diào)節(jié)細(xì)胞信號(hào)通路，進(jìn)而影響細(xì)胞的增殖、遷移和存活，目前在腫瘤等疾病的治療研究中受關(guān)注。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，該產(chǎn)品適用于體外研究NEO1在細(xì)胞遷移、組織修復(fù)模型中的調(diào)控機(jī)制，或用于評(píng)估藥物干預(yù)、基因編輯等實(shí)驗(yàn)條件下目標(biāo)蛋白的表達(dá)特征，為再生醫(yī)學(xué)基礎(chǔ)研究、疾病模型構(gòu)建及生物標(biāo)志物探索提供精準(zhǔn)的定量分析工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Immunoglobulin superfamily DCC subclass member 2 ELISA Kit; NEO1 ELISA Kit; NEO1_HUMAN ELISA Kit; Neogenin ELISA Kit; Ngn (gene name) ELISA Kit
  • 縮寫(xiě)：
    NEO1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Neuroscience
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NEO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 99
    Range % 94-104
    1:2 Average % 89
    Range % 83-97
    1:4 Average % 92
    Range % 87-97
    1:8 Average % 111
    Range % 106-114
  • 回收率：
    The recovery of human NEO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 87-99
    EDTA plasma (n=4) 105 103-111
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    v
    pg/ml OD1 OD2 Average Corrected
    5000 2.084 2.020 2.052 1.933
    2500 1.403 1.454 1.429 1.310
    1250 1.051 1.098 1.075 0.956
    625 0.712 0.721 0.717 0.598
    312 0.358 0.365 0.362 0.243
    156 0.245 0.254 0.250 0.131
    78 0.177 0.178 0.178 0.059
    0 0.115 0.122 0.119 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

What?type?is?the?standard?produced?by??

A:
Thanks for your inquiry!The standard in this kit is NS0-derived recombinant protein

靶點(diǎn)詳情

  • 功能：
    Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response.
  • 基因功能參考文獻(xiàn)：
    1. Overexpression of Neo1 significantly reduced the GC cell sensitivity to cisplatin and increased the cell viability, motility and adhesion ability, and while silencing of Neo1 showed contrary results. PMID: 30064133
    2. Endogenous NTN4/NEO1 maintain neuroblastoma growth via both pro-survival and pro-migratory molecular signaling. PMID: 28038459
    3. Environmental and endogenous proteases may contribute to cancer development by depleting DCC and neogenin. PMID: 27716118
    4. these results suggested that GD3 recruits gamma-secretase to lipid/rafts, allowing efficient cleavage of neogenin. PMID: 27288875
    5. The hemizygous 15q deletion unmasks the recessive functional polymorphism in NEO1 which plays a pivotal role in cortical interneuron development; study provides the first evidence linking NEO1 with autism spectrum disorders in humans PMID: 26518331
    6. Neogenin influences both cadherin dynamics and junctional tension. PMID: 27029596
    7. Ntn4 promotes the proliferation and motility of gastric cancer cells which is mediated by its receptor Neo and through further activation of multi-oncogenic pathways PMID: 25909166
    8. The present study demonstrates that neogenin may be a tumor suppressor in breast cancer. Neogenin may serve as a potential diagnostic marker and therapeutic target for breast cancer. PMID: 25998984
    9. These results show how repulsive guidance molecule acts as the central hub that links BMP2 and NEO1 and physically connects these fundamental signaling pathways. PMID: 25938661
    10. Neogenin expression is inversely associated with breast cancer grade. PMID: 25416629
    11. high-expression of neogenin-1 promotes gastric cancer proliferation and motility PMID: 24930499
    12. miR-18a may regulate biological behavior of human glioblastoma cells by targeting neogenin. PMID: 24657544
    13. these results highlight Neogenin1 as a novel downstream effector of the Sonic Hedgehog pathway in medulloblastoma PMID: 23775842
    14. two RGMB ectodomains conformationally stabilize the juxtamembrane regions of two NEO1 receptors in a pH-dependent manner. PMID: 23744777
    15. neogenin forms a ternary complex with both MT2 and HJV at the plasma membrane. The complex facilitates HJV cleavage by MT2, and release of the cleaved HJV from the cell occurs after a retrograde trafficking through the TGN/Golgi compartments. PMID: 22893705
    16. pattern of distribution suggests that a functional netrin-4-neogenin pathway might be restricted to syncytiotrophoblasts, mesenchymal cells, and villous endothelial cells PMID: 22705235
    17. the proposed role of neogenin as a tumor suppressor in gliomas PMID: 22666451
    18. identification of neogenin-binding site on the repulsive guidance molecule A PMID: 22396795
    19. Netrin-1 can promote the potential of proliferation and invasion of extravillous trophoblasts in vitro through its receptors neogenin and UNC5B. PMID: 19570425
    20. Neogenin FN5, which does not bind hemojuvelin in isolation, exhibits a highly electropositive surface, which may be involved in interactions with negatively-charged polysaccharides or phospholipids in the membrane bilayer. PMID: 20971194
    21. neogenin negatively regulates the functions of BMP and that this effect of neogenin is mediated by the activation of RhoA. PMID: 21149453
    22. results show that different isoforms of hemojuvelin may play unique physiological roles through defined interactions with distinct signaling proteins such as BMP2 and neogenin, and demonstrate that some of these interactions are defective. PMID: 18287331
    23. This study supports the hypothesis that, as in the embryo, neogenin regulates fundamental signalling pathways important for neurogenesis in the adult mouse and human forebrain. PMID: 20575069
    24. neogenin and restin have roles in proproliferation/survival action on ovarian cancer cells PMID: 12833147
    25. neogenin may play a role in mammary carcinogenesis as well as morphogenesis PMID: 16324219
    26. HJV-induced BMP signaling and hepcidin expression are not altered by neogenin overexpression or by inhibition of endogenous neogenin expr PMID: 18326817
    27. the neogenin intracellular domain regulates gene transcription via nuclear translocation PMID: 18391016
    28. neogenin-mediated HJV release occurs after the HJV-neogenin complex is internalized from the cell surface PMID: 18445598
    29. Results show that the serine/threonine kinase death-associated protein kinase (DAPK) is involved in the signal transduction of neogenin. PMID: 18583991
    30. Results support that hepatic neogenin possesses two functions, mediation of cellular HJV release, and stimulation of HJV-enhanced hepcidin expression. PMID: 19564337

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily, DCC family
  • 組織特異性：
    Widely expressed and also in cancer cell lines.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 7754

    OMIM: 601907

    KEGG: hsa:4756

    STRING: 9606.ENSP00000261908

    UniGene: Hs.388613