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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human Nesfatin-1 ELISA Kit
  • 中文名稱：
    人神經(jīng)肽Nesfatin-1酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15050h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人神經(jīng)肽Nesfatin-1酶聯(lián)免疫試劑盒（CSB-E15050h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Nesfatin-1含量。Nesfatin-1是一個(gè)值得關(guān)注的靶點(diǎn)。它在體內(nèi)廣泛分布，與能量代謝、進(jìn)食調(diào)節(jié)等生理過(guò)程密切相關(guān)。目前研究聚焦于其作用機(jī)制，如通過(guò)與特定受體結(jié)合，影響神經(jīng)信號(hào)傳導(dǎo)，調(diào)節(jié)代謝相關(guān)激素分泌，為肥胖、代謝疾病治療帶來(lái)新思路。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，適用于代謝疾病模型研究、肥胖相關(guān)神經(jīng)內(nèi)分泌機(jī)制探索，或通過(guò)檢測(cè)腦組織、外周血等樣本動(dòng)態(tài)觀察Nesfatin-1在應(yīng)激狀態(tài)下的表達(dá)變化，為研究能量代謝調(diào)控、神經(jīng)肽信號(hào)通路提供可靠工具，滿足基礎(chǔ)科研中對(duì)低豐度靶標(biāo)的高效檢測(cè)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    Nesfatin-1
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %96
    Range %80-105
    1:10Average %99
    Range %91-111
    1:20Average %104
    Range %92-112
    1:40Average %93
    Range %86-100
  • 回收率：
    The recovery of human Nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9790-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.617 2.557 2.587 2.463
    10002.205 2.356 2.281 2.157
    5001.636 1.767 1.702 1.578
    2501.043 1.058 1.051 0.927
    1250.609 0.642 0.626 0.502
    62.50.352 0.346 0.349 0.225
    31.250.226 0.243 0.235 0.111
    00.122 0.125 0.124 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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