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  4. Human Neuroblastoma suppressor of tumorigenicity 1(NBL1) ELISA kit

Human Neuroblastoma suppressor of tumorigenicity 1(NBL1) ELISA kit

  • 中文名稱：
    人神經(jīng)母細(xì)胞瘤致瘤性抑制因子1(NBL1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL015485HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人神經(jīng)母細(xì)胞瘤致瘤性抑制因子1(NBL1)酶聯(lián)免疫試劑盒（CSB-EL015485HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NBL1含量。試劑盒檢測范圍為34.4 pg/mL-2200 pg/mL，該產(chǎn)品適用于體外培養(yǎng)細(xì)胞模型、動物組織樣本及體液標(biāo)本的NBL1蛋白表達(dá)量檢測，可支持腫瘤生物學(xué)、神經(jīng)科學(xué)領(lǐng)域的基礎(chǔ)研究，以及藥物干預(yù)實(shí)驗(yàn)中生物標(biāo)志物的動態(tài)監(jiān)測，為探索NBL1在疾病發(fā)生發(fā)展中的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    D1S1733E ELISA Kit; DAN ELISA Kit; DAN domain family member 1 ELISA Kit; DAND 1 ELISA Kit; DAND1 ELISA Kit; Differential screening selected gene aberrant in neuroblastoma ELISA Kit; MGC8972 ELISA Kit; NB ELISA Kit; NBL 1 ELISA Kit; NBL1 ELISA Kit; NBL1_HUMAN ELISA Kit; Neuroblastoma candidate region suppression of tumorigenicity 1 ELISA Kit; Neuroblastoma suppression of tumorigenicity 1 ELISA Kit; Neuroblastoma suppressor of tumorigenicity 1 ELISA Kit; NO 3 ELISA Kit; NO3 ELISA Kit; Protein N03 ELISA Kit; Zinc finger protein DAN ELISA Kit
  • 縮寫：
    NBL1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    34.4 pg/mL-2200 pg/mL
  • 靈敏度：
    8.6 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human NBL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 84-96
    1:2 Average % 97
    Range % 91-105
    1:4 Average % 103
    Range % 97-107
    1:8 Average % 89
    Range % 83-94
  • 回收率：
    The recovery of human NBL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 94-105
    EDTA plasma (n=4) 106 101-110
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2200 1.755 1.821 1.788 1.665
    1100 1.285 1.289 1.287 1.164
    550 0.848 0.845 0.847 0.724
    275 0.547 0.533 0.540 0.417
    137.5 0.353 0.365 0.359 0.236
    68.75 0.298 0.321 0.310 0.187
    34.4 0.225 0.214 0.220 0.097
    0 0.125 0.121 0.123 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    DAN family
  • 組織特異性：
    Most abundant in normal lung and meningioma.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 7650

    OMIM: 600613

    KEGG: hsa:100532736

    STRING: 9606.ENSP00000289749

    UniGene: Hs.466662