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Human Neuropeptide-like protein C4orf48(C4orf48) ELISA kit

  • 中文名稱:
    人神經(jīng)肽樣蛋白C4orf48(C4orf48)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL003989HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人神經(jīng)肽樣蛋白C4orf48(C4orf48)酶聯(lián)免疫試劑盒(CSB-EL003989HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的C4orf48含量。C4orf48是一種位于人類4號(hào)染色體上的基因,編碼一種神經(jīng)肽樣蛋白。該蛋白在神經(jīng)系統(tǒng)的發(fā)育中表達(dá),可能與智力障礙和神經(jīng)退行性疾病有關(guān)。近期研究發(fā)現(xiàn),C4orf48通過RNA結(jié)合機(jī)制促進(jìn)腎臟纖維化,成為慢性腎臟病治療的新靶點(diǎn)。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL,適用于基礎(chǔ)研究領(lǐng)域探索C4orf48在神經(jīng)系統(tǒng)發(fā)育、神經(jīng)退行性疾病模型中的表達(dá)調(diào)控,或結(jié)合組織樣本分析其區(qū)域性分布特征??蒲腥藛T可通過檢測(cè)體液或組織裂解液中的蛋白含量,研究其在特定生理病理過程中的潛在作用機(jī)制,為神經(jīng)生物學(xué)研究提供實(shí)驗(yàn)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    C4orf48 ELISA Kit; Chr4_55 ELISA Kit; Neuropeptide-like protein C4orf48 ELISA Kit
  • 縮寫:
    C4orf48
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human C4orf48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %88-97
    1:2Average %95
    Range %90-100
    1:4Average %93
    Range %87-98
    1:8Average %103
    Range %97-108
  • 回收率:
    The recovery of human C4orf48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8681-92
    EDTA plasma (n=4)10297-107
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.564 2.689 2.627 2.494
    202.185 2.077 2.131 1.998
    101.459 1.524 1.492 1.359
    50.812 0.828 0.820 0.687
    2.50.423 0.432 0.428 0.295
    1.250.283 0.294 0.289 0.156
    0.6250.186 0.192 0.189 0.056
    00.132 0.134 0.133
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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