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  4. Human Obestatin ELISA Kit

Human Obestatin ELISA Kit

  • 中文名稱：
    人肥胖抑制素酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12818h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人肥胖抑制素酶聯(lián)免疫試劑盒（CSB-E12818h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的Obestatin含量。Obestatin是一種由胃部合成的23個氨基酸組成的酰胺化腦腸肽，與孤兒G蛋白偶聯(lián)受體GPR39結(jié)合，具有抑制食欲、減緩體重增加、抑制胃排空和小腸收縮的生物學(xué)功能。Obestatin與Ghrelin同源于Ghrelin基因，但作用相反，Obestatin可能通過GPR39受體調(diào)節(jié)胃腸動力。試劑盒檢測范圍為4.69 pg/mL-300 pg/mL，本試劑盒適用于體外科研場景，例如探究肥胖相關(guān)代謝疾病機制、胃腸功能調(diào)控或內(nèi)分泌信號通路研究，研究者可通過標(biāo)準(zhǔn)曲線精確測定樣本中Obestatin的動態(tài)濃度變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    Obestatin
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, urine, tissue homogenates
  • 檢測范圍：
    4.69 pg/mL-300 pg/mL
  • 靈敏度：
    1.17 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess. 
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess. 
     
  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human Obestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

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  • 回收率：

    The recovery of human Obestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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