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Human Orexin B,OX-B ELISA Kit

  • 中文名稱(chēng):
    人食欲素/阿立新B(OX-B)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E09863h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人食欲素/阿立新B(OX-B)酶聯(lián)免疫試劑盒(CSB-E09863h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的OX-B含量。人食欲素/阿立新B(OX-B)是一種由下丘腦神經(jīng)元分泌的神經(jīng)肽,能促進(jìn)食欲、調(diào)節(jié)睡眠和覺(jué)醒。研究發(fā)現(xiàn),OX-B與食欲素受體結(jié)合后,可調(diào)節(jié)攝食行為、睡眠周期及情緒等生理過(guò)程。針對(duì)OX-B及其受體的研究,為治療肥胖、失眠等疾病提供了新的治療靶點(diǎn)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL,該產(chǎn)品適用于神經(jīng)生物學(xué)研究中食欲素系統(tǒng)的功能解析、代謝調(diào)節(jié)機(jī)制探索,或睡眠障礙動(dòng)物模型中的生物標(biāo)志物分析,為中樞神經(jīng)系統(tǒng)調(diào)控研究提供可靠的檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    OX-B
  • 種屬:
    Homo sapiens (Human)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Metabolism
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human OX-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 107    
    Range % 95-111    
    1:2 Average % 100    
    Range % 93-104    
    1:4 Average % 94    
    Range % 86-97    
    1:8 Average % 100    
    Range % 92-104    
  • 回收率:
    The recovery of human OX-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 91 84-95    
    EDTA plasma (n=4) 108 97-112    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 2.312 2.378 2.345 2.212    
    2000 1.386 1.346 1.366 1.233    
    1000 0.820 0.812 0.816 0.683    
    500 0.523 0.504 0.514 0.381    
    250 0.370 0.356 0.363 0.230    
    125 0.278 0.268 0.273 0.140    
    62.5 0.209 0.203 0.206 0.073    
    0 0.132 0.134 0.133      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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