Human P53 antibody ELISA Kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人P53抗體酶聯(lián)免疫試劑盒（CSB-E13816h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的P53 Ab含量。P53是重要的抑癌基因，P53 Ab即P53抗體。其背景在于P53功能異常與多種腫瘤發(fā)生發(fā)展密切相關(guān)。研究機(jī)制方面，聚焦于P53 Ab與P53蛋白結(jié)合，以此監(jiān)測(cè)P53狀態(tài)，對(duì)于研究腫瘤發(fā)生機(jī)制、判斷預(yù)后及開(kāi)發(fā)治療策略有重要意義。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，適用于科研場(chǎng)景中定量分析P53在腫瘤機(jī)制研究、藥物作用評(píng)估或細(xì)胞應(yīng)激反應(yīng)中的表達(dá)水平變化，為癌癥生物學(xué)、分子病理學(xué)等領(lǐng)域的研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

P53 Ab
種屬：

Homo sapiens (Human)
樣本類(lèi)型：

serum, plasma
檢測(cè)范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human P53 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-105
1:2	Average %	94
	Range %	84-99
1:4	Average %	99
	Range %	90-101
1:8	Average %	92
	Range %	82-105

回收率：

The recovery of human P53 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-96
EDTA plasma (n=4)	94	87-103

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.