Human Pancreatic Amylase,PAMY ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

人胰淀粉酶(PAMY)酶聯(lián)免疫試劑盒（CSB-E09691h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AMY2A含量。AMY2A是一種編碼α-淀粉酶的基因，主要在胰腺中表達。它通過水解寡糖和多糖中的1,4-α-葡萄糖苷鍵，催化淀粉和糖原的消化。研究顯示，AMY2A與胰島素抵抗和糖尿病有關，其表達水平與血糖水平相關。近期研究揭示了AMY2A在淀粉消化和血糖調(diào)節(jié)中的重要作用，為糖尿病治療提供了新的靶點。試劑盒檢測范圍為47 mU/mL-3000 mU/mL，該產(chǎn)品適用于科研場景中胰腺外分泌功能評估、消化系統(tǒng)疾病機制研究或藥物干預效果分析，例如在實驗動物模型或體外細胞模型中追蹤PAMY表達動態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

AMY2A ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
縮寫：

AMY2A
Uniprot No.：

P04746
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

47 mU/mL-3000 mU/mL
靈敏度：

11.75 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PAMY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
Range %	89-99
1:2	Average %	102
Range %	96-107
1:4	Average %	94
Range %	87-98
1:8	Average %	86
Range %	81-92

回收率：

The recovery of human PAMY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-103
EDTA plasma (n=4)	102	96-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.