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  4. Human Peptidase inhibitor 16(PI16) ELISA kit

Human Peptidase inhibitor 16(PI16) ELISA kit

  • 中文名稱：
    人肽酶抑制劑16酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL017951HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人肽酶抑制劑16酶聯(lián)免疫試劑盒（CSB-EL017951HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PI16含量。人肽酶抑制劑16是一種新型肽-肽酶抑制劑結(jié)合物，通過(guò)共價(jià)連接肽和肽酶抑制劑，延長(zhǎng)肽的血清半衰期，提高藥物療效。其作用機(jī)制主要是通過(guò)抑制特定肽酶活性，延長(zhǎng)肽類(lèi)藥物在體內(nèi)的作用時(shí)間，從而提高治療效果。試劑盒檢測(cè)范圍為0.312 ng/mL-10 ng/mL，該產(chǎn)品廣泛應(yīng)用于基礎(chǔ)科研領(lǐng)域，如炎癥性疾病機(jī)制研究、感染模型中的宿主防御反應(yīng)分析、皮膚或肺組織修復(fù)過(guò)程中的蛋白動(dòng)態(tài)監(jiān)測(cè)等，為探索Elafin在先天免疫調(diào)控及病理生理過(guò)程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Cripi ELISA Kit; CRISP 9 ELISA Kit; CRISP-9 ELISA Kit; CRISP9 ELISA Kit; Cysteine rich protease inhibitor ELISA Kit; Cysteine rich secretory protein 9 ELISA Kit; Cysteine-rich secretory protein 9 ELISA Kit; Microseminoprotein beta binding protein ELISA Kit; MSMBBP ELISA Kit; Peptidase inhibitor 16 ELISA Kit; PI-16 ELISA Kit; PI16 ELISA Kit; PI16_HUMAN ELISA Kit; Protease inhibitor 16 ELISA Kit; PSP94 binding protein ELISA Kit; PSP94-binding protein ELISA Kit; PSPBP ELISA Kit
  • 縮寫(xiě)：
    PI16
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-10 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cell Biology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<20%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PI16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 91
    Range % 83-98
    1:2 Average % 95
    Range % 88-105
    1:4 Average % 90
    Range % 84-105
    1:8 Average % 95
    Range % 89-100
  • 回收率：
    The recovery of human PI16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-105
    EDTA plasma (n=4) 95 86-108
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　
    10 2.254 2.266 2.260 　
    5 1.342 1.354 1.348 　
    2.5 0.662 0.669 0.666 　
    0.625 0.197 0.211 0.204 　
    0.312 0.099 0.102 0.101 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May inhibit cardiomyocyte growth.
  • 基因功能參考文獻(xiàn)：
    1. at high endothelial shear stress, PI16 contributes to inhibition of MMP2 protease activity, protection that can be reversed during inflammation PMID: 27996045
    2. PI16-positive Treg showed enhanced in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation PMID: 22533972
    3. A new blood protein PSP94-binding protein (PSPBP) is identified that binds prostate secretory protein of 94 amino acids (PSP94). PMID: 15344909
    4. a novel and independent prognostic markers following radical prostatectomy for prostate cancer. PMID: 17062675
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    CRISP family
  • 組織特異性：
    Expressed in prostate, testis, ovary and intestine. Concentrates in prostate cancer patient's sera.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 21245

    KEGG: hsa:221476

    STRING: 9606.ENSP00000362778

    UniGene: Hs.25391