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  4. Human Peptidyl-prolyl cis-trans isomerase C(PPIC) ELISA kit

Human Peptidyl-prolyl cis-trans isomerase C(PPIC) ELISA kit

  • 中文名稱：
    人肽脯氨酰順反異構(gòu)酶C(PPIC)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL018473HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人肽脯氨酰順反異構(gòu)酶C(PPIC)酶聯(lián)免疫試劑盒（CSB-EL018473HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PPIC含量。人肽脯氨酰順反異構(gòu)酶C（PPIC）是一種在蛋白質(zhì)折疊和成熟中起關(guān)鍵作用的酶，通過催化脯氨酸酰亞胺肽鍵的順反異構(gòu)化，確保蛋白質(zhì)正確折疊和成熟，對細胞蛋白質(zhì)穩(wěn)態(tài)至關(guān)重要。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，適用于基礎(chǔ)科研中PPIC相關(guān)疾病機制探索、細胞模型或動物模型的蛋白表達動態(tài)分析，以及藥物干預(yù)實驗中的生物標(biāo)志物定量評估。可為分子生物學(xué)、病理學(xué)及藥理學(xué)研究提供可靠的數(shù)據(jù)支持，滿足科研工作者對PPIC蛋白定量分析的多樣化需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cyclophilin C ELISA Kit; CYPC ELISA Kit; MGC3673 ELISA Kit; Parvulin ELISA Kit; Peptidyl prolyl cis trans isomerase C ELISA Kit; Peptidyl-prolyl cis-trans isomerase C ELISA Kit; Peptidylprolyl isomerase C (cyclophilin C) ELISA Kit; peptidylprolyl isomerase C ELISA Kit; PPIase C ELISA Kit; ppiC ELISA Kit; PPIC_HUMAN ELISA Kit; Rotamase C ELISA Kit
  • 縮寫：
    PPIC
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PPIC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-104
    1:2 Average % 91
    Range % 82-98
    1:4 Average % 103
    Range % 98-108
    1:8 Average % 87
    Range % 82-98
  • 回收率：
    The recovery of human PPIC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 85-98
    EDTA plasma (n=4) 111 105-115
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.651 2.656 2.654 2.497
    750 2.322 2.251 2.287 2.130
    375 1.645 1.655 1.650 1.493
    187.5 1.002 0.974 0.988 0.831
    94 0.587 0.544 0.566 0.409
    47 0.389 0.401 0.395 0.238
    23.5 0.262 0.245 0.254 0.097
    0 0.156 0.157 0.157 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.
  • 基因功能參考文獻：
    1. Independent validation with glioma patients tissue (n = 70) and normal brain tissue (n = 19) found PPIC, EMP3 and CHI3L1 were up-regulated in glioma tissue. Survival value validation showed that the three genes correlated with patient survival by Kaplan-Meir analysis, including grades, age and therapy. PMID: 27801851
    2. Cyclophilin C participates in cytomegalovirus US2 protein-mediated degradation of major histocompatibility complex class I molecules. PMID: 26691022
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Cyclophilin-type PPIase family
  • 組織特異性：
    Expressed in kidney, skeletal muscle, pancreas, heart, lung, liver and to a lower extent in brain.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9256

    OMIM: 123842

    KEGG: hsa:5480

    STRING: 9606.ENSP00000303057

    UniGene: Hs.110364