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  4. Human Phosphatidylinositol-glycan-specific phospholipase D(GPLD1) ELISA kit

Human Phosphatidylinositol-glycan-specific phospholipase D(GPLD1) ELISA kit

  • 中文名稱：
    人磷脂酰肌醇聚糖特異性磷脂酶D(GPLD1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL009721HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人磷脂酰肌醇聚糖特異性磷脂酶D(GPLD1)酶聯免疫試劑盒（CSB-EL009721HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GPLD1含量。GPLD1（Glycosylphosphatidylinositol-specific phospholipase D1）是一種參與多種生物學過程的酶，包括脂質運輸、病原體控制和糖尿病等。其基因敲除小鼠模型已被構建，用于研究其在小鼠體內的功能。研究顯示，GPLD1的表達與阿爾茨海默?。ˋD）模型中β-淀粉樣蛋白的產生相關，可能通過調節(jié)GAL3BP的表達來影響AD的發(fā)生。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于基礎科研領域中對GPLD1表達動態(tài)的定量分析，例如探究其在代謝性疾病、神經系統(tǒng)疾病或腫瘤微環(huán)境中的分子機制。提供預包被板條和即用型試劑，操作流程標準化，支持批量樣本檢測，廣泛用于細胞實驗、動物模型研究以及生物標志物篩選等場景，為研究GPLD1相關生理病理功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Glycoprotein phospholipase D ELISA Kit; Glycosyl-phosphatidylinositol-specific phospholipase D ELISA Kit; GPI-PLD ELISA Kit; GPI-specific phospholipase D ELISA Kit; GPIPLD ELISA Kit; GPIPLDM ELISA Kit; GPLD1 ELISA Kit; MGC22590 ELISA Kit; PHLD_HUMAN ELISA Kit; Phosphatidylinositol glycan specific phospholipase D ELISA Kit; Phosphatidylinositol-glycan-specific phospholipase D ELISA Kit; PI-G PLD ELISA Kit; PIGPLD ELISA Kit; PIGPLD1 ELISA Kit
  • 縮寫：
    GPLD1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GPLD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %90
    Range %84-96
    1:400Average %97
    Range %91-103
    1:800Average %103
    Range %97-109
    1:1600Average %89
    Range %83-95
  • 回收率：
    The recovery of human GPLD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10094-106
    EDTA plasma (n=4)9591-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    2002.841 2.752 2.797 2.673
    1002.564 2.402 2.483 2.359
    502.135 1.965 2.050 1.926
    251.358 1.355 1.357 1.233
    12.50.691 0.744 0.718 0.594
    6.250.371 0.395 0.383 0.259
    3.120.225 0.226 0.226 0.102
    00.125 0.123 0.124
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
  • 基因功能參考文獻：
    1. computational study suggests that although curcumin to some extent binds with Tp receptor, yet the inhibition of Arf6GDP to Arf6GTP conversion appeared to be an important mechanism by which curcumin inhibits U46619-induced increase in PLD activity in PASMCs. PMID: 28780751
    2. Study discovered novel and independent associations of prediabetes and related traits with MASP1, and some evidence for associations with THBS1, GPLD1 and ApoA-IV, suggesting a role for these proteins in the pathophysiology of type 2 diabetes. PMID: 27344311
    3. c-Myc influences GPI-AP signaling transcriptionally and posttranslational and represses GPI-AP anti-proliferative signaling in tumors PMID: 27232633
    4. Low aggressive MCF-7 breast cancer cells have low endogenous PLD enzymatic activity and cell invasion, concomitant with high expression of miR-203, -887, and -3619 and miR-182 and miR-182. PMID: 26567912
    5. suggest that the down-regulation of GPI-PLD protein may be involved in prion propagation in the brains of prion diseases PMID: 25867459
    6. our findings showed that ANRIL is an lncRNA responsible in anti-tumorigenesis caused by PLD inhibition and combined incorporation of ANRIL into PLD inhibition-induced anti-tumorigenic signaling network PMID: 25964559
    7. AMPK suppresses PLD activity, and PLD suppresses AMPK via mTOR. PMID: 25632961
    8. Functional regulation of phospholipase D expression in cancer and inflammation. PMID: 24990948
    9. At the cellular level, PLD and its reaction product, phosphatidate, interact with a large number of protein partners that are directly related to the actin cytoskeleton and cell migration. PMID: 24990954
    10. PLD1 and PLD2 mutants inhibit very-low-density lipoprotein-induced aldosterone production in HAC15 cells. PMID: 24956203
    11. This study indicated that PLD participates in the same signaling pathway in this breast cancer cell line. PMID: 22481092
    12. genetic variation of GPLD1 appears to associate with circulating glycosylphosphatidylinositol-specific phospholipase D levels PMID: 22260953
    13. The mRNA expression and activity of GPI-PLD in de novo and refractory or relapsed acute myeloid leukemia patients are obviously higher than those in normal controls. PMID: 20137110
    14. This study revealed the novel mechanism of bleomycin-induced redox-sensitive activation of phospholipase D leading to the generation of phosphaticid acid. PMID: 21131602
    15. The role of phospholipase D in the transport of VAT1 to plasma membranes and in phosphatidic acid metabolism in neutrophils is reported. PMID: 20858461
    16. In human ovarian cancer cell lines, GPI-PLD mRNA expression correlated with GPI-PLD enzyme activity and with the shedding of the GPI-anchored tumor and prognostic markers, urokinase receptor and CA125, from the cell surface. PMID: 12090469
    17. increased serum glycosylphosphatidylinositol-specific phospholipase D is associated with insulin resistance in a cohort with a wide range of insulin sensitivity PMID: 14767861
    18. identified as component of human trypanosome lytic factor 1 (TLF1) PMID: 15500911
    19. PLD activation is a novel finding for the D(3) receptor, and is the first example of an effector system where D(3) signals without G(i)/G(o) protein intermediates. PMID: 15500962
    20. CTLA-4 may be stored in a specialized compartment in regulatory T cells that can be triggered rapidly for deployment to the plasma membrane in a phospholipase D- and ADP ribosylation factor-1-dependent manner PMID: 15814706
    21. Data describe the role of angiotensin II type 1A receptor phosphorylation, phospholipase D, and extracellular calcium in isoform-specific protein kinase C membrane translocation responses. PMID: 16831865
    22. GPI-PLD probably had no contribution to hematopoietic stem cell homing, which may due to its low or no expression in cord blood, bone marrow and mobilized peripheral blood CD34(+) cells. PMID: 17467053
    23. Data indicate that a critical component of Ras signaling is the activation of PLD. PMID: 17949898
    24. We show that human PLD1b and PLD2a contain functional caspase 3 cleavage sites and identify the critical aspartate residues within PLD1b that affect its activation by phorbol esters. PMID: 18298948
    25. The serum GPI-PLD activities, the protein and mRNA levels of GPI-PLD in hepatocellular carcinoma patients were decreased by 40%, 60% and 56%, respectively. PMID: 19135435

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    GPLD1 family
  • 數據庫鏈接：

    HGNC: 4459

    OMIM: 602515

    KEGG: hsa:2822

    STRING: 9606.ENSP00000230036

    UniGene: Hs.533291