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  4. Human Phospholipase A1 member A(PLA1A) ELISA kit

Human Phospholipase A1 member A(PLA1A) ELISA kit

  • 中文名稱：
    人磷脂酶A1成員A(PLA1A) 酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL018084HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人磷脂酶A1成員A(PLA1A) 酶聯(lián)免疫試劑盒（CSB-EL018084HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PLA1A含量。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，該產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域，如探究PLA1A在脂代謝相關(guān)疾病模型中的動(dòng)態(tài)變化、評(píng)估其在炎癥反應(yīng)中的作用機(jī)制，或用于細(xì)胞及動(dòng)物實(shí)驗(yàn)中蛋白表達(dá)水平的定量比較分析，為脂質(zhì)代謝與相關(guān)疾病的分子機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    AA986889 ELISA Kit; NMD ELISA Kit; OTTHUMP00000215248 ELISA Kit; OTTHUMP00000215249 ELISA Kit; OTTHUMP00000215250 ELISA Kit; Phosphatidylserine specific phospholipase A1 ELISA Kit; Phosphatidylserine specific phospholipase A1alpha ELISA Kit; Phosphatidylserine-specific phospholipase A1 ELISA Kit; Phospholipase A1 member A ELISA Kit; pla1a ELISA Kit; PLA1A_HUMAN ELISA Kit; PS PLA1 ELISA Kit; PS-PLA1 ELISA Kit; PSPLA 1 ELISA Kit; PSPLA1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLA1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 88    
    Range % 82-95    
    1:2 Average % 97    
    Range % 93-101    
    1:4 Average % 99    
    Range % 95-106    
    1:8 Average % 93    
    Range % 87-99    
  • 回收率：
    The recovery of human PLA1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 91 87-95    
    EDTA plasma (n=4) 99 95-104    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    20 2.340 2.344 2.342 2.237    
    10 1.604 1.741 1.673 1.568    
    5 0.936 0.968 0.952 0.847    
    2.5 0.576 0.554 0.565 0.460    
    1.25 0.353 0.325 0.339 0.234    
    0.625 0.230 0.213 0.222 0.117    
    0.312 0.185 0.180 0.183 0.078    
    0 0.104 0.106 0.105 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Hydrolyzes the ester bond of the acyl group attached at the sn-1 position of phosphatidylserines (phospholipase A1 activity) and 1-acyl-2-lysophosphatidylserines (lysophospholipase activity) in the pathway of phosphatidylserines acyl chain remodeling. Cleaves phosphatidylserines exposed on the outer leaflet of the plasma membrane of apoptotic cells producing 2-acyl-1-lysophosphatidylserines, which in turn enhance mast cell activation and histamine production. Has no activity toward other glycerophospholipids including phosphatidylcholines, phosphatidylethanolamines, phosphatidic acids or phosphatidylinositols, or glycerolipids such as triolein.; Hydrolyzes lyso-PS but not PS.
  • 基因功能參考文獻(xiàn)：
    1. Serum PS-PLA1 levels were significantly higher in the melanoma subjects and associated with the clinical stages. PMID: 29500864
    2. the LysoPA levels might be determined largely by LysoPC and LysoPI (possible precursors) and the PS-PLA1-mediated pathway might be involved in the production of LysoPS in gastric cancer. PMID: 28143894
    3. PS-PLA1 expression in colorectal cancer is associated with tumor invasion and metastasis. PMID: 25750298
    4. These data suggest that PLA1A plays an important role in bridging the membrane-associated NS2-E2 complex and the NS5A-associated replication complex via its interaction with hepatitis C virus E2, NS2, and NS5A. PMID: 25505071
    5. PLA1A2 polymorphism is associated with mortality in participants whose hemoglobin A1c ranges from 5.5% to 6.5%. PMID: 24886443
    6. Overall, our study shed the light on new structural features of the phospholipase activity of pancreatic lipase family members. PMID: 24368210
    7. These results suggest that the expression of PS-PLA(1) mRNA in THP-1-derived macrophages is activated via TLR4. PMID: 20573295
    8. polymorphisms in the gene encoding phosphatidylserine-specific phospholipase A1 PMID: 12436198

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 組織特異性：
    Widely expressed. Expressed in placenta, prostate and liver. Weakly or not expressed in skin, leukocytes, platelets, colon, spleen, lung, muscle and kidney.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 17661

    OMIM: 607460

    KEGG: hsa:51365

    STRING: 9606.ENSP00000273371

    UniGene: Hs.437451