Human Plastin-2(LCP1) ELISA kit
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中文名稱:人網(wǎng)素-2(LCP1)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL012824HU
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人網(wǎng)素-2(LCP1)酶聯(lián)免疫試劑盒(CSB-EL012824HU)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿.樣本中的LCP1含量。人網(wǎng)素-2(LCP1)是一種肌動(dòng)蛋白結(jié)合調(diào)節(jié)因子,在T細(xì)胞激活中發(fā)揮關(guān)鍵作用,通過(guò)TCR/CD3和CD2或CD28響應(yīng)共刺激信號(hào),調(diào)節(jié)IL2RA/CD25和CD69的細(xì)胞表面表達(dá),并參與免疫反應(yīng)和T細(xì)胞激活。其研究機(jī)制涉及與AIF1和肌動(dòng)蛋白的相互作用,以及調(diào)節(jié)細(xì)胞表面信號(hào)分子的表達(dá)。試劑盒檢測(cè)范圍為15.6 pg/ml-1000 pg/ml,該產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域,如探究LCP1在免疫細(xì)胞功能、腫瘤微環(huán)境調(diào)控或炎癥性疾病模型中的表達(dá)特征,亦可結(jié)合其他分子標(biāo)記物進(jìn)行多維度機(jī)制研究,為體外實(shí)驗(yàn)及動(dòng)物模型樣本分析提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:CP64 ELISA Kit; L plastin ELISA Kit; L-plastin ELISA Kit; Larval cuticle protein 1 ELISA Kit; LC64P ELISA Kit; LCP-1 ELISA Kit; LCP1 ELISA Kit; LPL ELISA Kit; Lplastin ELISA Kit; Lymphocyte cytosolic protein 1 ELISA Kit; Plastin 2 ELISA Kit; Plastin-2 ELISA Kit; PLS2 ELISA Kit; PLSL_HUMAN ELISA Kit
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縮寫(xiě):LCP1
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates.
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檢測(cè)范圍:15.6 pg/ml-1000 pg/ml.
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靈敏度:3.9 pg/ml
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Signal Transduction
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human LCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
106
Range %
102-112
1:2
Average %
96
Range %
90-102
1:4
Average %
98
Range %
91-105
1:8
Average %
91
Range %
87-96
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回收率:
The recovery of human LCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
92
88-97
EDTA plasma (n=4)
99
92-106
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
1.998
1.874
1.936
1.813
500
1.264
1.257
1.260
1.137
250
0.841
0.828
0.834
0.711
125
0.518
0.529
0.523
0.400
62.5
0.383
0.391
0.387
0.264
31.2
0.295
0.283
0.289
0.166
15.6
0.199
0.189
0.194
0.071
0
0.122
0.125
0.123
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal diseases MM Grant,Journal of clinical periodontology,2022
- The actin-bundling protein L-plastin: a novel local inflammatory marker associated with periodontitis. ?zt眉rk V?.et al,J Periodontal Res.,2015
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
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基因功能參考文獻(xiàn):
- LCP1-positive oral squamous cell carcnome samples were correlated closely with the primary tumor size and regional lymph node metastasis. PMID: 28230172
- MOLP8/R cells display a very high overexpression of LCP1 gene (l-Plastin) controlled by HIF1&alpha. PMID: 29882856
- these findings support a plausible mechanism by which the AP4/L-plastin axis is regulated by the PI3K/AKT pathway in human prostate cancer (PCa)and may represent a novel therapeutic target in PCa treatment. PMID: 28981098
- Mutated LCP1 is a driver of chronic lymphocytic leukemia. PMID: 28679620
- AngII-dependent phosphorylation of LCP1 in cultured podocytes was mediated by the kinases ERK, p90 ribosomal S6 kinase, PKA, or PKC. LCP1 phosphorylation increased filopodia formation. PMID: 28768720
- L-plastin regulates the stability of the immune synapse of naive and effector T-cells. (Review) PMID: 27720134
- The findings support a mechanism in which miR-375 suppresses RUNX1 levels, resulting in reduced vimentin and L-plastin expression. Knockdown of RUNX1, L-plastin, and vimentin resulted in significant reductions in cell invasion in vitro, indicating the functional significance of miR-375 regulation of specific proteins involved in head and neck squamous cell carcinoma (HNSCC) invasion. PMID: 28499703
- In this study, the authors found that the actin filament bundling abilities of PLS1 and PLS2 were similarly sensitive to Ca(2+) (pCa50 ~6.4), whereas PLS3 was less sensitive (pCa50 ~5.9). PMID: 28694070
- elevated L-plastin expression promotes elongation and reduces protrusion density in cells with relatively lower L-plastin than fascin levels. PMID: 26945069
- Enhanced nitroxidative stress may results in LPL S-glutathionylation leading to impaired chemotaxis, polarization, and bactericidal activity of human neutrophils. PMID: 25881549
- association of SNPs in LCP1 and CTIF with hearing PMID: 26264041
- An NKX3.1 binding site polymorphism in the l-plastin promoter leads to differential gene expression in human prostate cancer PMID: 26148677
- The proteins (HSP90b, TSM1 and L-plastin) in the current study may hold potential in differentiating between melanoma and benign nevi in diagnostically challenging cases. PMID: 25191796
- Data suggest that several single-nucleotide polymorphisms (SNPs) of the plastin genes PLS3 and LCP1 could serve as gender- and/or stage-specific molecular predictors of tumor recurrence in stage II/III colorectal cancer as well as therapeutic targets. PMID: 24170770
- L-plastin plays an important role in the clustering of NKG2D into lipid rafts, and it participates in NKG2D-mediated inhibition of NK cell chemotaxis. PMID: 24803550
- expression of L-plastin promotes tumor metastasis and, importantly, that this effect depends on an additionally required phosphorylation of L-plastin PMID: 24438191
- L-plastin is indispensable for podosome formation and function in macrophages. PMID: 24236012
- LCP1 is functionally relevant to CXCL12 induced B-cell migration. PMID: 24009233
- High serum LCP1 is associated with kidney cancer. PMID: 23479363
- Hepatic LCP1 mRNA was increased (by 300%) in liver biopsy samples from patients with nonalcoholic fatty liver disease compared to controls PMID: 23213074
- This study adds L-plastin to a growing list of proteins implicated in T lymphocyte polarity and migration PMID: 22581862
- our data introduce costimulation-induced L-plastin phosphorylation as an important event for immune synapse formation and its inhibition by dexamethasone as a novel mode of function of this immunosuppressive glucocorticoid. PMID: 21805466
- Results establish a causative role for PKCbetaII and L-plastin in linking GM-CSF-induced eosinophil priming for chemotaxis. PMID: 21525390
- Plasmic L-plastin level in patients with colorectal cancer was higher than that in healthy adults, and was associated with tumor size, penetration, and lymphatic metastasis. PMID: 20878578
- required for immune synapse formation PMID: 20683899
- This study discloses a novel unexpected role of the actin bundling protein L-plastin as a cell protective protein against TNF-cytotoxicity. PMID: 19799649
- Data demonstrate for the first time that L-plastin contributes to the fine-tuning of actin turn-over, an activity which is regulated by Ser5 phosphorylation promoting its high affinity binding to the cytoskeleton. PMID: 20169155
- Data show that the serine protease plasmin cleaved both propeptides from human vascular endothelial growth factor (VEGF)-D, generating mature forms, and also activated VEGF-C. PMID: 12963694
- association of L-plastin overexpression with increased rate of proliferation and invasion, and loss of E-cadherin expression in the SW480 colon cancer cell line indicates that L-plastin plays an important mechanistic role in colorectal cancer metastasis PMID: 16287074
- Data suggest that phosphorylated L-plastin might act as an integrator of signals controlling the assembly of the actin cytoskeleton and cell motility in a 3D-space. PMID: 16636079
- Data show that an increase in melanoma cell invasiveness requires not only expression but also phosphorylation of L-plastin. PMID: 17290393
- Phosphorylation of the actin bundling protein L-plastin represents a mechanism by which costimulation controls the transport of activation receptors to the T cell surface. PMID: 17294403
- L-plastin and S100A9 were differentially expressed in nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissue PMID: 19142861
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相關(guān)疾病:Chromosomal aberrations involving LCP1 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;13)(q27;q14), with BCL6.
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亞細(xì)胞定位:Cytoplasm, cytoskeleton. Cell junction. Cell projection. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
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組織特異性:Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
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