Human Platelet-Derived Growth Factor AA(PDGF-AA) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

人血小板衍生生長因子AA(PDGF-AA)酶聯(lián)免疫試劑盒（CSB-E17143h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-AA含量。PDGF - AA 即血小板衍生生長因子 AA 型，是一種重要的細胞因子。它在細胞增殖、分化等過程中發(fā)揮關(guān)鍵作用，與組織修復(fù)、血管生成等生理過程相關(guān)。研究機制主要圍繞其與受體結(jié)合，激活下游信號通路，在腫瘤、纖維化等疾病發(fā)病機制中有重要研究價值。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于心血管疾病機制研究、組織再生模型分析、抗纖維化藥物開發(fā)等科研領(lǐng)域；為探索 PDGF - AA 在炎癥反應(yīng)、胚胎發(fā)育或創(chuàng)傷愈合中的功能提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PDGF-AA
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-102
1:2	Average %	100
	Range %	92-104
1:4	Average %	92
	Range %	85-96
1:8	Average %	94
	Range %	85-99

回收率：

The recovery of human PDGF-AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	104	95-110

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.