Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

人前心鈉肽(Pro-ANP)酶聯(lián)免疫試劑盒（CSB-E05149h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Pro-ANP含量。Pro-ANP（前心鈉素原）是一個具有重要生理意義的靶點。它可反映心臟功能狀態(tài)，與心血管系統(tǒng)疾病密切相關。研究機制上，主要聚焦于其在調節(jié)體液平衡、血壓穩(wěn)定等方面的作用，探尋其在疾病發(fā)生發(fā)展中的變化，為相關疾病治療提供理論依據(jù)。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品廣泛應用于心血管疾病機制研究、藥物干預效果評估、動物模型心臟功能分析等科研場景，尤其適用于探索心力衰竭、高血壓及相關代謝性疾病中Pro - ANP的動態(tài)變化規(guī)律，為實驗室提供穩(wěn)定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Pro-ANP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	85
	Range %	80-89
1:4	Average %	92
	Range %	87-98
1:8	Average %	104
	Range %	96-108

回收率：

The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	87	82-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.