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  4. Human Proadrenomedullin (Pro-ADM) ELISA Kit

Human Proadrenomedullin (Pro-ADM) ELISA Kit

  • 中文名稱：
    人腎上腺髓質(zhì)素前體(Pro-ADM)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14356h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人腎上腺髓質(zhì)素前體(Pro-ADM)酶聯(lián)免疫試劑盒（CSB-E14356h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的Pro-ADM含量。人腎上腺髓質(zhì)素前體（Pro-ADM）是一種由多種組織產(chǎn)生的肽，具有穩(wěn)定微循環(huán)和防止內(nèi)皮通透性的作用。其在膿毒癥、下呼吸道感染等疾病中顯示出作為治療監(jiān)測和風(fēng)險評估的潛力，其水平與疾病嚴(yán)重程度和后續(xù)不良事件的可能性相關(guān)。試劑盒檢測范圍為0.312 pmol/L-20 pmol/L，該產(chǎn)品支持多種生物樣本類型，能夠滿足基礎(chǔ)醫(yī)學(xué)研究中疾病機制探索、藥物作用評估或生物標(biāo)志物篩選等需求，例如通過檢測循環(huán)系統(tǒng)中Pro - ADM的動態(tài)變化，為心血管疾病或全身性炎癥相關(guān)研究提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    Pro-ADM
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, urine, tissue homogenates
  • 檢測范圍：
    0.312 pmol/L-20 pmol/L
  • 靈敏度：
    0.078 pmol/L
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:200Average %85
    Range %80-92
    1:400Average %95
    Range %90-100
    1:800Average %99
    Range %92-110
    1:1600Average %92
    Range %82-105
  • 回收率：
    The recovery of human Pro-ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-100
    EDTA plasma (n=4)10190-110
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/LOD1OD2AverageCorrected
    203.052 2.952 3.002 2.860
    102.587 2.476 2.532 2.390
    51.819 1.704 1.762 1.620
    2.51.125 1.109 1.117 0.975
    1.250.702 0.684 0.693 0.551
    0.6250.406 0.389 0.398 0.256
    0.3120.273 0.258 0.266 0.124
    00.147 0.137 0.142 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價